Source	Chinese Hamster Ovary cell line, CHO-derived mouse Neprilysin/CD10 protein Tyr52-Trp750, with an N-terminal 5-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His
Predicted Molecular Mass	81 kDa
SDS-PAGE	97 kDa, under reducing conditions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005). The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1126-ZNC

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl₂.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

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Background: Neprilysin/CD10

Neprilysin, (NEP, neutral endopeptidase 24.11, EC 3.4.24.11), is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states (3, 4). These and other observations have resulted in considerable interest in NEP as a target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer's disease by promoting A beta accumulation (5).

Item	Value
References	Malfroy, B. et al. (1978) Nature 276:523. Kenny, A.J. and Stephenson, S.L. (1988) FEBS Lett. 232:1. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247. Shipp, M.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4819. Itwata, N. et al. (2001) Science 292:1550.
Entrez Gene IDs	4311 (Human); 17380 (Mouse); 24590 (Rat); 101925772 (Cynomolgus Monkey)
Alternate Names	Atriopeptidase, CALLA, CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase), CD10, CD10 antigen, CD10), CD10membrane metallo-endopeptidase variant 1, Common acute lymphocytic leukemia antigen, DKFZp686O16152, EC 3.4.24, EC 3.4.24.11, EPN, Enkephalinase, Leu-19, MGC126681, MGC126707, MME, NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, NKH1, Neprilysin, Neutral Endopeptidase 24.11, Neutral endopeptidase, SFE, Skin fibroblast elastase, membrane metallo-endopeptidase, membrane metallo-endopeptidase variant 2

Citations for Recombinant Mouse Neprilysin (CHO-expressed) Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Neprilysin (CHO-expressed) Protein, CF include:

Species: Mouse
Sample Types: Protein
Applications: Bioassay

TD Madsen et al. (2020), An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles Nat Commun, 11(1):4033.
PMID: 32820167

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Recombinant Mouse Neprilysin (CHO-expressed) Protein, CF

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