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Recombinant Mouse Neprilysin (CHO-expressed) Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1126-ZNC

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1126-ZNC-010

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Neprilysin/CD10 protein
Tyr52-Trp750, with an N-terminal 5-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

81 kDa

SDS-PAGE

97 kDa, under reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

1126-ZNC
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Neprilysin/CD10

Neprilysin, (NEP, neutral endopeptidase 24.11, EC 3.4.24.11), is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states (3, 4). These and other observations have resulted in considerable interest in NEP as a target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer's disease by promoting A beta accumulation (5).

References

  1. Malfroy, B. et al. (1978) Nature 276:523.
  2. Kenny, A.J. and Stephenson, S.L. (1988) FEBS Lett. 232:1.
  3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
  4. Shipp, M.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4819.
  5. Itwata, N. et al. (2001) Science 292:1550.

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Entrez Gene IDs

4311 (Human); 17380 (Mouse); 24590 (Rat); 101925772 (Cynomolgus Monkey)

Gene Symbol

MME

UniProt

Additional Neprilysin/CD10 Products

Product Documents for Recombinant Mouse Neprilysin (CHO-expressed) Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Neprilysin (CHO-expressed) Protein, CF

For research use only

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