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Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10739-CV

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10739-CV-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mers-cov Spike protein
MERS-CoV Spike
(Tyr18-Lys1294)(Arg748Ser, Arg751Ser, Val1060Pro, Leu1061Pro)
Accession # YP_007188579.1
GCN4-IZ 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr18

Predicted Molecular Mass

146 kDa

SDS-PAGE

145-175 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA with Recombinant Human DPPIV/CD26 (High Purity Dimer) (Catalog # 9168-SE).

Scientific Data Images for Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein, CF

Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein Binding Activity.

Recombinant MERS-CoV Spike (GCN4-IZ) His-tag (Catalog # 10739-CV) binds Recombinant Human DPPIV/CD26 (High Purity Dimer) (9168-SE) in a functional ELISA.

Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant MERS-CoV Spike (GCN4-IZ) His-tag (Catalog # 10739-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 145-175 kDa.

Formulation, Preparation and Storage

10739-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike

MERS-CoV (also known as HCoV-EMC), which causes the Middle East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that also include SARS-CoV-1 and SARS-CoV-2. Coronaviruses are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). MERS-CoV Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry, and it consists of two subunits, S1 and S2. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3). The MERS-CoV S protein shares 31% and 29% amino acid (aa) sequence identity with SARS-CoV S1 subunit and SARS-Cov2 S1 subunit, respectively. The low aa sequence homology is consistent with the finding that MERS-CoV and SARS-CoV bind different cellular receptors (4). Unlike SARS-CoV and SARS-CoV2, which engage ACE-2 as their receptors for cell entry, MERS-CoV employs Dipeptidyl Peptidase 4 (DPP4; also known as CD26) as its functional receptor (4). Based on structural biology studies, the receptor binding domain (RBD) of MERS-CoV spike protein is located in the C-terminal region of S1 subunit and consists of a core subdomain and a receptor-binding subdomain (5, 6). The S1 subunit, especially the RBD region, was commonly targeted for vaccinations or antiviral therapy against MERS (7-9).

References

  1. Bermingham, A. et al. (2012) Euro Surveill. 17:20290.
  2. Zaki, A.M. et al. (2012) N. Engl. J. Med. 367:1814.
  3. Li, Y. et al. (2019) Engineering. 5:940.
  4. Raj, V.S. et al. (2013) Nature 495:251.
  5. Lu, G. et al. (2013) Nature 500:227.
  6. Wang, N. et al. (2013) Cell. Res. 23:986.
  7. Corti, D. et al. (2016) J. Infect. Public Health 9:231.
  8. Tang, X.C. et al. (2014) Proc. Natl. Acad. Sci. USA 111:E2018.
  9. Jiang, L. et al. (2014) Sci. Transl. Med. 6:234ra59.

Long Name

Spike Protein

Alternate Names

S Protein

Entrez Gene IDs

918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

Additional Spike Products

Product Documents for Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein, CF

For research use only

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