Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein, CF

Name: Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein, CF
Brand: R&D Systems
SKU: 9168-SE

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9168-SE

Citations (11)

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Key Product Details

Source:	NS0
Accession #:	CAA43118
Structure / Form:	Noncovalently-linked homodimer
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein, CF

Source	Mouse myeloma cell line, NS0-derived human DPPIV/CD26 protein Asn29-Pro766, with a C-terminal Asp-Ile and 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asn29
Predicted Molecular Mass	86 kDa
SDS-PAGE	95-110 kDa, reducing condtions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/µg as measured under the described conditions.

Scientific Data Examples for Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein, CF

	Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein Enzyme Activity Recombinant Human DPPIV/CD26 (Catalog # 9168-SE) is measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The activity (orange) is approximately 4-fold greater than the competitor's DPPIV/CD26 (green).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9168-SE

Formulation	Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is an approximately 110 kDa serine exopeptidase that releases Xaa-Pro or Xaa-Ala dipeptides from the N-terminus of oligo- and polypeptides. It regulates immune and endocrine function through the cleavage of multiple chemokines, growth factors, and peptide hormones (1, 2). Mature human DPPIV consists of a 6 amino acid (aa) cytoplasmic tail, a 22 aa transmembrane segment, and a 738 aa extracellular domain (ECD) that contains the catalytic active site (Ser, Asp, and His charge relay system) (3). Within the ECD, human DPPIV/CD26 shares 84% amino acid sequence identity with mouse and rat DPPIV. DPPIV is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes, and it can be released by MMP mediated shedding (4). It cleaves a range of peptide hormones including Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P (5). It is released from adipocytes and induces insulin resistance in adipocytes and skeletal muscle (6). DPPIV also cleaves many chemokines, resulting in reduced chemotactic activity of CXCL6, 9, 10, 11, 12, and CCL5 (7-10) but unchanged angiostatic activity of CXCL9 and CXCL10 (8). Cleavage can increase (CCL5), decrease (CXCL12), or have no effect (CCL4) on chemokine blockade of HIV-1 cellular infectivity (7, 9, 11). In addition, DPPIV cleavage of CCL4 broadens chemokine receptor usage to also include CCR2b (11). DPPIV serves as a cell entry coreceptor for HIV and coronavirus (12, 13). It cleaves human GM-CSF and IL-3 and reduces their ability to promote myeloid cell development (14). It also interferes with CXCL12 induced hematopoietic cell migration, homing, and engraftment (15). DPPIV interacts in cis with adenosine deaminase on T cells and in trans with caveolin-1 on antigen presenting cells (16, 17). It provides costimulatory proliferation and activation signals to both CD4⁺ and CD8+ T cells (17, 18).

Item	Value
References	Klemann, C. et al. (2016) Clin. Exp. Immunol. Epub PMID 26919392. Mortier, A. et al. (2016) J. Leukoc. Biol. Epub PMID 26744452. Tanaka, T. et al. (1992) J. Immunol. 149:481. Rohrborn, D. et al. (2014) FEBS Lett. 588:3870. Waumans, Y. et al. (2015) Front. Immunol. 6:387. Lamers, D. et al. (2011) Diabetes 60:1917. Proost, P. et al. (1998) J. Biol. Chem. 273:7222. Proost, P. et al. (2001) Blood 98:3554. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236. Barreira da Silva, R. et al. (2015) Nat. Immunol. 16:850. Guan, E. et al. (2002) J. Biol. Chem. 277:32348. Callebaut, C. et al. (1993) Science 262:2045. Raj, V.S. et al. (2013) Nature 495:251. Broxmeyer, H.E. et al. (2012) Nat. Med. 18:1786. Christopherson II, K.W. et al. (2004) Science 305:1000. Kameoka, J. et al. (1993) Science 261:466. Ohnuma, K. et al. (2007) J. Biol. Chem. 282:10117. Hatano, R. et al. (2013) Immunology 138:165.
Long Name	Dipeptidyl-peptidase IV
Entrez Gene IDs	1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)
Alternate Names	ADABP, ADCP-2, ADCP2DPP IV, Adenosine deaminase complexing protein 2TP103, CD26, CD26 antigen, CD26T-cell activation antigen CD26, DPP4, DPPIV, Dipeptidyl peptidase IV, EC 3.4.14.5, dipeptidyl peptidase 4, dipeptidyl-peptidase 4, dipeptidylpeptidase 4, dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)

Citations for Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein, CF (11)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human DPPIV/CD26 (High Purity Dimer) Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Gao et al. (2021), Continuous stimulation of dual-function peptide PGLP-1-VP inhibits the morbidity and mortality of NOD mice through anti-inflammation and immunoregulation Scientific Reports, 11(1):3593.
PMID: 33574570
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

XY Chen et al. (2021), SDF-1 inhibits the dedifferentiation of islet beta cells in hyperglycaemia by up-regulating FoxO1 via binding to CXCR4 Journal of Cellular and Molecular Medicine, 0(0).
PMID: 34935260
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

A Valverde et al. (2021), Dipeptidyl peptidase 4 contributes to Alzheimer's disease-like defects in a mouse model and is increased in sporadic Alzheimer's disease brains The Journal of Biological Chemistry, 297(2):100963.
PMID: 34265307
Species: N/A

Applications: Bioassay

JM Cho et al. (2020), Discovery of a novel fibroblast activation protein (FAP) inhibitor, BR103354, with anti-diabetic and anti-steatotic effects Scientific Reports, 10(1):21280.
PMID: 33277568
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Nishina et al. (2019), Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice Cell Mol Gastroenterol Hepatol, 7(1):115-134.
PMID: 30510994
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Gao et al. (2018), Pharmacological Effects of EGLP-1, a Novel Analog of Glucagon-Like Peptide-1, on Carbohydrate and Lipid Metabolism Cell. Physiol. Biochem., 48(3):1112-1122.
PMID: 30041175
Species: Virus
Sample Types: Pseudotype Virus
Applications: Bioassay

KS Inn et al. (2018), Reduction of soluble dipeptidyl peptidase 4 levels in plasma of patients infected with Middle East respiratory syndrome coronavirus Virology, 518(0):324-327.
PMID: 29587190
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

M Chen et al. (2017), Pericyte-targeting prodrug overcomes tumor resistance to vascular disrupting agents J. Clin. Invest., 0(0).
PMID: 28846068
Species: Human
Sample Types: Plasma
Applications: ELISA (Standard)

DI Bromage et al. (2017), A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies PLoS ONE, 12(4):e0174447.
PMID: 28379992
Sample Types: Protein
Applications: Bioassay

Joshua R Willard (2016), Improved glycaemia in high-fat-fed neprilysin-deficient mice is associated with reduced DPP-4 activity and increased active GLP-1 levels Diabetologia, 0(0).
PMID: 27933334
Species: Human
Sample Types: Whole Cells

Tai W et al. (0), Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine. Cell Mol Immuno17(6):613-620.
PMID: 32203189

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