Source	Human embryonic kidney cell, HEK293-derived human PDGF R beta protein Leu33-Lys531, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu33
Predicted Molecular Mass	57 kDa
SDS-PAGE	93-103 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human PDGF R beta His-tag protein is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human PDGF-BB (Catalog # 220-BB) binds with an ED₅₀ of 0.5-3 ng/mL.

Recombinant Human PDGF R beta His-tag Protein, CF Scientific Data Examples

	Recombinant Human PDGF R beta His-tag Protein Binding Activity. When Recombinant Human PDGF R beta His-tag (Catalog # 10676-PR) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human PDGF-BB (220-BB) binds with an ED₅₀ of 0.5-3 ng/mL.
	Recombinant Human PDGF R beta His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human PDGF R beta His-tag (Catalog # 10676-PR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 93-103 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10676-PR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF R beta

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or heterodimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. The extracellular domain of human PDGF R beta contains 4 disulfide bonds and shares a 79% sequence identity with mouse and rat PDGF R beta. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages). A soluble PDGF R alpha has been detected in normal human plasma and serum as well as in the conditioned medium of the human osteosarcoma cell line MG-63 (5). Both the recombinant mouse and human soluble PDGF R alpha bind PDGF with high affinity and are potent PDGF antagonists.

Item	Value
References	Betshotz, C. et al. (2001) BioEssays 23:494. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517. Tiesman, J. and C.E. Hart (1993) J. Biol. Chem. 5:9621.
Long Name	Platelet-derived Growth Factor Receptor beta
Entrez Gene IDs	5159 (Human); 18596 (Mouse); 24629 (Rat)
Alternate Names	CD140 antigen-like family member B, CD140b, CD140b antigen, EC 2.7.10, EC 2.7.10.1, JTK12, PDGF R beta, PDGF-R-beta, PDGFR, PDGFR1, PDGFRB, beta-type platelet-derived growth factor receptor, platelet-derived growth factor receptor, beta polypeptide

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Recombinant Human PDGF R beta His-tag Protein, CF

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