Recombinant Human/Mouse FGF-8b Protein

Name: Recombinant Human/Mouse FGF-8b Protein
Brand: R&D Systems
SKU: 423-F8
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 423-F8

Citations (57)

(2)

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Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	NP_006110 (human), NP_001159834 (mouse)
Applications:	Bioactivity

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Product Specifications for Recombinant Human/Mouse FGF-8b Protein

Source	E. coli-derived FGF-8 protein Gln23-Arg215, with an N-terminal Met
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	22.5 kDa
SDS-PAGE	23 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is typically 6.5-40 ng/mL in the presence of 1 µg/mL heparin.

Scientific Data Examples for Recombinant Human/Mouse FGF-8b Protein

	Recombinant Human/Mouse FGF-8b Protein Bioactivity Recombinant Human/Mouse FGF-8b (Catalog # 423-F8) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED₅₀ for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.
	Recombinant Human/Mouse FGF-8b Protein SDS-PAGE 1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

423-F8

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and Brij-35 with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after reconstitution.

423-F8/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and Brij-35.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-8

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

Item	Value
References	Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928. Gemel, J. et al. (1996) Genomics 35:253. Olsen, S.K. et al. (2006) Genes Dev. 20:185. Crossley, P.H. et al. (1996) Cell, 84:127. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129. Sun, X. et al. (1999) Genes Dev. 13:1834. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425. Mattila, M.M. et al. (2001) Oncogene 20:2791. Valve, E. et al. (2000) Int. J. Cancer 88:718. Valve, E.M. et al. (2001) Lab. Invest. 81:815. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.
Long Name	Fibroblast Growth Factor 8
Entrez Gene IDs	2253 (Human); 14179 (Mouse); 29349 (Rat)
Alternate Names	AIGF, AIGFKAL6, Androgen-induced growth factor, FGF-8, FGF8, HBGF-8, Heparin-binding growth factor 8, MGC149376, fibroblast growth factor 8, fibroblast growth factor 8 (androgen-induced)

Citations for Recombinant Human/Mouse FGF-8b Protein (57)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human/Mouse FGF-8b Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Yu et al. (2022), Increased Expression and Altered Cellular Localization of Fibroblast Growth Factor Receptor-Like 1 (FGFRL1) Are Associated with Prostate Cancer Progression Cancers, 14(2).
PMID: 35053442
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

H Ozaki et al. (2022), Differentiation of human induced pluripotent stem cells into hypothalamic vasopressin neurons with minimal exogenous signals and partial conversion to the naive state Scientific Reports, 12(1):17381.
PMID: 36253431
Species: Mouse
Sample Types: Whole Cell
Applications: Cell Culture

DS Lee et al. (2022), Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells Stem Cells International, 2022(0):1092184.
PMID: 36213683
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Restan Per et al. (2021), 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink Biomolecules, 11(8).
PMID: 34439916
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

T Otsuka et al. (2021), Control of mesenchymal cell fate via application of FGF-8b in vitro Stem Cell Research, 51(0):102155.
PMID: 33445073
Species: Huiman
Sample Types: Whole Cells
Applications: Bioassay

C Zhou et al. (2021), FGF8 and BMP2 mediated dynamic regulation of dental mesenchyme proliferation and differentiation via Lhx8/Suv39h1 complex Journal of Cellular and Molecular Medicine, 0(0).
PMID: 33580754
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

N Hosaka et al. (2021), Induction of gammadeltaT cells from HSC-enriched BMCs co-cultured with iPSC-derived thymic epithelial cells Journal of Cellular and Molecular Medicine, 0(0).
PMID: 34687276
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

TW Kim et al. (2021), Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use Cell Stem Cell, 28(2):343-355.e5.
PMID: 33545081
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

H Gla beta et al. (2020), Combined Dendritic and Axonal Deterioration Are Responsible for Motoneuronopathy in Patient-Derived Neuronal Cell Models of Chorea-Acanthocytosis Int J Mol Sci, 21(5).
PMID: 32151030
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KX Liang et al. (2020), Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations EMBO Mol Med, 0(0):e12146.
PMID: 32840960
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KX Liang et al. (2020), Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations EMBO Mol Med, 0(0):e12146.
PMID: 32840960
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JL Goggi et al. (2020), Dopamine transporter neuroimaging accurately assesses the maturation of dopamine neurons in a preclinical model of Parkinson's disease Stem Cell Res Ther, 11(1):347.
PMID: 32771055
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Elkouris et al. (2019), Long Non-coding RNAs Associated With Neurodegeneration-Linked Genes Are Reduced in Parkinson's Disease Patients Front Cell Neurosci, 13(0):58.
PMID: 30853899
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

I Allodi et al. (2019), Modeling Motor Neuron Resilience in ALS Using Stem Cells Stem Cell Reports, 0(0).
PMID: 31080111
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

S Mahajani et al. (2019), Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors Cell Death Dis, 10(12):898.
PMID: 31776327
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Westphal et al. (2019), Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes Sci Rep, 9(1):8470.
PMID: 31186471
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Li et al. (2018), Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages Cell Transplant, 0(0):9636897187803.
PMID: 29895168
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

G Collo et al. (2018), Alpha6-Containing Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Structural Plasticity in Mouse and Human iPSC-Derived Dopaminergic Neurons Front Pharmacol, 9(0):572.
PMID: 29910731
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Hu et al. (2018), Efficient induction of functional ameloblasts from human keratinocyte stem cells Stem Cell Res Ther, 9(1):126.
PMID: 29720250
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

G Shall et al. (2018), Effects of Passage Number and Differentiation Protocol on the Generation of Dopaminergic Neurons from Rat Bone Marrow-Derived Mesenchymal Stem Cells Int J Mol Sci, 19(3).
PMID: 29498713
Species: Human
Sample Types: Whole Cells
Applications: Differentiation

G Collo et al. (2018), Ropinirole and Pramipexole Promote Structural Plasticity in Human iPSC-Derived Dopaminergic Neurons via BDNF and mTOR Signaling Neural Plast., 2018(0):4196961.
PMID: 29531524
Species: Human
Sample Types: Whole Cells
Applications: Differentiation

A Ruzo et al. (2018), Chromosomal instability during neurogenesis in Huntington's disease Development, 145(2).
PMID: 29378824
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SA Liddelow et al. (2017), Neurotoxic reactive astrocytes are induced by activated microglia Nature, 541(7638):481-487.
PMID: 28099414
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Johansen et al. (2017), Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells Stem Cells Int, 2017(0):9605432.
PMID: 29201062
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

AR Rodrigues et al. (2017), Integration of Shh and Fgf signaling in controlling Hox gene expression in cultured limb cells Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28270602
Species: Human
Sample Types: Protein
Applications: Bioassay

(2016), Fibulin-1 Binds to Fibroblast Growth Factor 8 with High Affinity: Effects on Embryo Survival J Biol Chem, 0(0).
PMID: 27402846
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Daniel A Monti (2016), N-Acetyl Cysteine May Support Dopamine Neurons in Parkinson's Disease: Preliminary Clinical and Cell Line Data PLoS ONE, 11(6):e0157602.
PMID: 27309537
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Eva Hedlund (2016), Dopamine Receptor Antagonists Enhance Proliferation and Neurogenesis of Midbrain Lmx1a-expressing Progenitors Sci Rep, 6(0):26448.
PMID: 27246266
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Florian Wegner (2016), Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization J. Neurosci., 36(47):12027-12043.
PMID: 27881786
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Chan W et al. (2015), 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline. PLoS ONE, 10(6):e0130147.
PMID: 26075383
Species: Chicken
Sample Types: In Vivo
Applications: Bioassay

Attia L et al. (2015), Collective cell migration of the nephric duct requires FGF signaling. Dev Dyn, 244(2):157-67.
PMID: 25516335
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Shen et al. (2014), Susceptibility of human embryonic stem cell-derived neural cells to Japanese encephalitis virus infection. PLoS ONE, 9(12):e114990.
PMID: 25517725
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lewandowski J et al. (2014), Manipulating gene expression and signaling activity in cultured mouse limb bud cells. Dev Dyn, 243(7):928-36.
PMID: 24633820
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Mong J et al. (2014), Transcription factor-induced lineage programming of noradrenaline and motor neurons from embryonic stem cells. Stem Cells, 32(3):609-22.
PMID: 24549637
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

Yamamoto-Shiraishi Y et al. (2014), Etv1 and Ewsr1 cooperatively regulate limb mesenchymal Fgf10 expression in response to apical ectodermal ridge-derived fibroblast growth factor signal. Dev Biol, 394(1):181-90.
PMID: 25109552
Species: Zebrafish
Sample Types: Whole Cells
Applications: In Vivo

McCarroll et al. (2013), Fgf3 and Fgf10a work in concert to promote maturation of the epibranchial placodes in zebrafish. PLoS ONE, 8(12):e85087.
PMID: 24358375
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Stappert L et al. (2013), MicroRNA-based promotion of human neuronal differentiation and subtype specification. PLoS ONE, 8(3):e59011.
PMID: 23527072
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ni et al. (2013), Self-assembling peptide nanofiber scaffolds enhance dopaminergic differentiation of mouse pluripotent stem cells in 3-dimensional culture. PLoS ONE, 8(12):e84504.
PMID: 24376815
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

Roelandt P et al. (2010), Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells. PLoS ONE, 5(8):e12101.
PMID: 20711405
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tomlinson DC et al. (2010), Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer. Am. J. Pathol., 177(5):2379-86.
PMID: 20889570
Species: Avian - Quail
Sample Types: In Vivo
Applications: In Vivo

Nimmagadda S et al. (2007), FGFs, Wnts and BMPs mediate induction of VEGFR-2 (Quek-1) expression during avian somite development. Dev. Biol., 305(2):421-9.
PMID: 17425953
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Salero E et al. (2007), Differentiation of ES cells into cerebellar neurons. Proc. Natl. Acad. Sci. U.S.A., 104(8):2997-3002.
PMID: 17293457
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lindsley RC et al. (2006), Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 133(19):3787-96.
PMID: 16943279
Species: Zebrafish
Sample Types: Whole Tissue
Applications: Bioassay

Shimizu T et al. (2006), Cdx-Hox code controls competence for responding to Fgfs and retinoic acid in zebrafish neural tissue. Development, 133(23):4709-19.
PMID: 17079270
Species: Chicken
Sample Types: In Vivo
Applications: In Vivo

Tiecke E et al. (2006), Expression of the short stature homeobox gene Shox is restricted by proximal and distal signals in chick limb buds and affects the length of skeletal elements. Dev. Biol., 298(2):585-96.
PMID: 16904661
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

Hutson MR et al. (2006), Cardiac arterial pole alignment is sensitive to FGF8 signaling in the pharynx. Dev. Biol., 295(2):486-97.
PMID: 16765936
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Yamashita H et al. (2006), Embryonic stem cell-derived neuron models of Parkinson's disease exhibit delayed neuronal death. J. Neurochem., 98(1):45-56.
PMID: 16805795
Species: Chicken
Sample Types: In Vivo
Applications: In Vivo

Delfini MC et al. (2005), Control of the segmentation process by graded MAPK/ERK activation in the chick embryo. Proc. Natl. Acad. Sci. U.S.A., 102(32):11343-8.
PMID: 16055560
Species: Xenopus
Sample Types: In Vivo
Applications: In Vivo

Reversade B et al. (2005), Depletion of Bmp2, Bmp4, Bmp7 and Spemann organizer signals induces massive brain formation in Xenopus embryos. Development, 132(15):3381-92.
PMID: 15975940
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Castelo-Branco G et al. (2005), Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion. Mol. Cell. Neurosci., 31(2):251-62.
PMID: 16243537
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Perrier AL et al. (2004), Derivation of midbrain dopamine neurons from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A., 101(34):12543-8.
PMID: 15310843
Species: Zebrafish
Sample Types: In Vivo
Applications: In Vivo

Wiellette EL et al. (2003), vhnf1 and Fgf signals synergize to specify rhombomere identity in the zebrafish hindbrain. Development, 130(16):3821-9.
PMID: 12835397
Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay

Gunhaga L et al. (2003), Specification of dorsal telencephalic character by sequential Wnt and FGF signaling. Nat. Neurosci., 6(7):701-7.
PMID: 12766771
Species: Xenopus
Sample Types: In Vivo
Applications: In Vivo

Mogi K et al. (2003), Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein. Int. J. Dev. Biol., 47(1):15-29.
PMID: 12653248
Species: Chicken
Sample Types: In Ovo
Applications: In Ovo

Alsan BH et al. (2002), Regulation of avian cardiogenesis by Fgf8 signaling. Development, 129(8):1935-43.
PMID: 11934859
Species: Rabbit
Sample Types: Whole Cells
Applications: Bioassay

Fischer A et al. (2002), FGF8 acts as a right determinant during establishment of the left-right axis in the rabbit. Curr. Biol., 12(21):1807-16.
PMID: 12419180
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Abu-Issa R et al. (2002), Fgf8 is required for pharyngeal arch and cardiovascular development in the mouse. Development, 129(19):4613-25.
PMID: 12223417

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Customer Reviews for Recombinant Human/Mouse FGF-8b Protein (2)

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Recombinant Human/Mouse FGF-8b Protein

Name: Sameehan Mahajani

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 05/26/2017

It would be helpful to have larger aliquots.
Recombinant Human/Mouse FGF-8b Protein

Name: Marco Onorati

Application: CellProlif

Verified Customer | Posted 08/03/2016

Recombinant Human/Mouse FGF-8b Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human/Mouse FGF-8b Protein

Scientific Data Examples for Recombinant Human/Mouse FGF-8b Protein

Formulation, Preparation and Storage

Carrier Free

423-F8

Carrier

423-F8/CF

Carrier Free

Reconstitution Calculator

Background: FGF-8

Citations for Recombinant Human/Mouse FGF-8b Protein (57)

Customer Reviews for Recombinant Human/Mouse FGF-8b Protein (2)

Recombinant Human/Mouse FGF-8b Protein

Recombinant Human/Mouse FGF-8b Protein

Product Documents for Recombinant Human/Mouse FGF-8b Protein

Product Datasheets

COA

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