Order Information
Shipping Information
$35 to United States
Products in stock will be shipped in 1-3 business days unless specified.
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, completed N-2 Plus-supplemented neural cell medium is prepared using the following procedure:
- Dilute the media supplement in basal media
- Store completed media
- Use within 2 weeks
Reagents provided in the N-2 Plus Media Supplement (Catalog # AR003):
- Bovine Insulin (2,500 µg/mL)
- Human Transferrin (10,000 µg/mL)
- Putrescine (1,611 µg/mL)
- Selenite (0.52 µg/mL)
- Progesterone (0.63 µg/mL)
Reagents
- DMEM/F-12 (Invitrogen®, Catalog # 12500-062) or a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029)
- Glucose
- Glutamine
- NaHCO3
- Penicillin-Streptomycin (100X)
- Deionized or distilled water
Materials
- Serological pipettes
- Pipettes and pipette tips
- 2 µm filter unit
Equipment
- 2 °C to 8 °C refrigerator
Option 1: Mix the following components with deionized or distilled water to make 500 mL of medium.
Component | Amount |
DMEM/F-12 | 6 g |
Glucose | 0.775 g |
Glutamine | 0.0365 g |
NaHCO3 | 0.854 g |
N-2 Plus Media Supplement | 5 mL |
Adjust the pH to 7.2. Filter the solution (2 µm filter unit), and add 5 mL of 100X sterile Penicillin-Streptomycin solution. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.
Option 2: Dilute 100-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.
Invitrogen is a registered trademark of Invitrogen Corp.
Citations (21)
Citations are publications that use Bio-techne products. Selected citations for N-2 Plus Media Supplement include:
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Species: Mouse
Sample Types: Whole Cells
T Kobayashi et al. (2019), Enhanced lysosomal degradation maintains the quiescent state of neural stem cells Nat Commun, 10(1):5446.
PMID: 31784517 -
Species: Rat
Sample Types: Whole Cells
Applications: Cell CultureJM Yi et al. (2019), Ameliorative effects of aqueous extract of Forsythiae suspensa fruits on oxaliplatin-induced neurotoxicity in vitro and in vivo BMC Complement Altern Med, 19(1):339.
PMID: 31783843 -
Species: Mouse
Sample Types: Whole Cells
J Wang et al. (2018), Zika virus infected primary microglia impairs NPCs proliferation and differentiation Biochem. Biophys. Res. Commun., 0(0):.
PMID: 29453985 -
Species: Mouse
Sample Types: Whole Tissue
Applications: BioassayJJ Brown et al. (2018), Reovirus-Induced Apoptosis in the Intestine Limits Establishment of Enteric Infection J. Virol., 0(0):.
PMID: 29514905 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayN Boukelmoun et al. (2018), Mitochondrial transfer from mesenchymal stem cells to neural stem cells protects against the neurotoxic effects of cisplatin Acta Neuropathol Commun, 6(1):139.
PMID: 30541620 -
Species: Mouse
Sample Types: Whole Cells
Mao S et al. (2014), miR-17 regulates the proliferation and differentiation of the neural precursor cells during mouse corticogenesis. FEBS J, 281(4):1144-58.
PMID: 24314167 -
Species: Human
Sample Types: Whole Cells
Iacopino F et al. (2014), Isolation of cancer stem cells from three human glioblastoma cell lines: characterization of two selected clones. PLoS ONE, 9(8):e105166.
PMID: 25121761 -
Species: Mouse
Sample Types: Whole Cells
Capaldo C et al. (2014), Proinflammatory cytokine-induced tight junction remodeling through dynamic self-assembly of claudins. Mol Biol Cell, 25(18):2710-9.
PMID: 25031428 -
Species: Mouse
Sample Types: Whole Tissue
Hu S et al. (2014), Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma. PLoS ONE, 9(8):e105219.
PMID: 25133679 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayNajm et al. (2013), Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol, 31(5):426-33.
PMID: 23584611 -
Species: Mouse
Sample Types: Whole Cells
Zhou et al. (2013), Induction of intestinal stem cells by R-spondin 1 and Slit2 augments chemoradioprotection. Nature, 501(7465):107-11.
PMID: 23903657 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayJustilien V et al. (2012), Matrix metalloproteinase-10 is required for lung cancer stem cell maintenance, tumor initiation and metastatic potential. PLoS ONE, 7(4):e35040.
PMID: 22545096 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySpence JR et al. (2011), Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 470(7332):105-9.
PMID: 21151107 -
Species: Human
Sample Types: Whole Cells
Yang MH et al. (2010), Bmi1 is essential in Twist1-induced epithelial-mesenchymal transition. Nat. Cell Biol., 12(10):982-92.
PMID: 20818389 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayChang CJ et al. (2009), Enhanced radiosensitivity and radiation-induced apoptosis in glioma CD133-positive cells by knockdown of SirT1 expression. Biochem. Biophys. Res. Commun., 380(2):236-42.
PMID: 19166820 -
Species: Human
Sample Types: Whole Cells
Salim C et al. (2009), The giant protein AHNAK involved in morphogenesis and laminin substrate adhesion of myelinating Schwann cells. Glia, 57(5):535-49.
PMID: 18837049 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayRao RC et al. (2008), Efficient Serum-Free Derivation of Oligodendrocyte Precursors from Neural Stem Cell-Enriched Cultures. Stem Cells, 0(0):.
PMID: 18403757 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayKang SM et al. (2006), Efficient induction of oligodendrocytes from human embryonic stem cells. Stem Cells, 25(2):419-24.
PMID: 17053214 -
Species: Mouse
Sample Types: Whole Cells
Yamashita H et al. (2006), Embryonic stem cell-derived neuron models of Parkinson's disease exhibit delayed neuronal death. J. Neurochem., 98(1):45-56.
PMID: 16805795 -
Species: Rat
Sample Types: Whole Cells
Soares S et al. (2005), Neuronal and glial expression of the adhesion molecule TAG-1 is regulated after peripheral nerve lesion or central neurodegeneration of adult nervous system. Eur. J. Neurosci., 21(5):1169-80.
PMID: 15813926 -
Species: Human
Sample Types: Whole Cells
Ni HT et al. (2004), High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells. Brain Res. Dev. Brain Res., 152(2):159-69.
PMID: 15351504
There are no citations that match your criteria.
Customer Reviews (2)
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N-2 Plus Media Supplement
Verified Customer | Posted 04/04/2018 -
N-2 Plus Media Supplement
Verified Customer | Posted 12/01/2017Great growth and stability of E14 Neuronal stem cells
There are no reviews that match your criteria.
FAQs
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What is the difference between N-2 Plus Media Supplement (Catalog # AR003) and N-2 MAX Media Supplement (Catalog # AR009)?
The N-2 Plus Media Supplement (Catalog # AR003) contains Bovine Insulin whereas N-2 MAX Media Supplement (Catalog # AR009) contains Recombinant Human Insulin. All other media components and concentrations in N-2 Plus and N-2 MAX are the same. These two products perform comparably in side-by-side testing. Due to a limited supply of bovine insulin, the products are priced differently.
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Can the N-2 MAX Media Supplement be thawed, aliquoted and refrozen to be used with smaller batches of basal media?
Yes, this will not affect the stability of the supplement. Limited freeze-thaw of the supplement to use it in small batches is acceptable.
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Can the N-2 Plus Media Supplement be thawed, aliquoted and refrozen to be used with smaller batches of basal media?
Yes, this will not affect the stability of the supplement. Limited freeze-thaw of the supplement to use it in small batches is acceptable.