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N-2 Plus Media Supplement

Neural Progenitor Cells Expanded with N-2 Plus Media Supplement Express Nestin and SOX2
(2)
Verification of Neural Progenitor Cell Multipotency Following Expansion with N-2 Plus Media Supplement
(2)
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, completed N-2 Plus-supplemented neural cell medium is prepared using the following procedure:

  • Dilute the media supplement in basal media
  • Store completed media
  • Use within 2 weeks
 

 

Reagents Provided

Reagents provided in the N-2 Plus Media Supplement (Catalog # AR003):

  • Bovine Insulin (2,500 µg/mL)
  • Human Transferrin (10,000 µg/mL)
  • Putrescine (1,611 µg/mL)
  • Selenite (0.52 µg/mL)
  • Progesterone (0.63 µg/mL)

 

Other Supplies Required

Reagents

  • DMEM/F-12 (Invitrogen®, Catalog # 12500-062) or a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029)
  • Glucose
  • Glutamine
  • NaHCO3
  • Penicillin-Streptomycin (100X)
  • Deionized or distilled water

Materials

  • Serological pipettes
  • Pipettes and pipette tips
  • 2 µm filter unit

Equipment

  • 2 °C to 8 °C refrigerator

 

Procedure Overview

Option 1: Mix the following components with deionized or distilled water to make 500 mL of medium.

Component Amount
DMEM/F-12 6 g
Glucose 0.775 g
Glutamine 0.0365 g
NaHCO3 0.854 g
N-2 Plus Media Supplement 5 mL

Adjust the pH to 7.2. Filter the solution (2 µm filter unit), and add 5 mL of 100X sterile Penicillin-Streptomycin solution. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

Option 2: Dilute 100-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

 

Invitrogen is a registered trademark of Invitrogen Corp.

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    Verified Customer | Posted 04/04/2018
  • N-2 Plus Media Supplement
    Name: Anonymous
    Verified Customer | Posted 12/01/2017
    Great growth and stability of E14 Neuronal stem cells
    N-2 Plus Media Supplement Serum AR003

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FAQs

  • What is the difference between N-2 Plus Media Supplement (Catalog # AR003) and N-2 MAX Media Supplement (Catalog # AR009)?

    The N-2 Plus Media Supplement (Catalog # AR003) contains Bovine Insulin whereas N-2 MAX Media Supplement (Catalog # AR009) contains Recombinant Human Insulin. All other media components and concentrations in N-2 Plus and N-2 MAX are the same. These two products perform comparably in side-by-side testing. Due to a limited supply of bovine insulin, the products are priced differently.

  • Can the N-2 MAX Media Supplement be thawed, aliquoted and refrozen to be used with smaller batches of basal media?

     Yes, this will not affect the stability of the supplement. Limited freeze-thaw of the supplement to use it in small batches is acceptable.

  • Can the N-2 Plus Media Supplement be thawed, aliquoted and refrozen to be used with smaller batches of basal media?

    Yes, this will not affect the stability of the supplement. Limited freeze-thaw of the supplement to use it in small batches is acceptable.

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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