Human Nestin Antibody

Name: Human Nestin Antibody
Brand: R&D Systems
SKU: MAB1259
Rating: 4 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1259

Citations (99)

(4)

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Key Product Details

Species Reactivity:	Human
Immunogen:	NS0 mouse myeloma cell line transfected with human Nestin
Applications:	CyTOF-reported, Flow Cytometry, Immunocytochemistry/Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 196908
Specificity:	Detects human Nestin.

View Full Technical Details for Human Nestin Antibody

Applications for Human Nestin Antibody

	Recommended Concentration	Sample
CyTOF-reported	This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/10⁶ cells	A172 human glioblastoma cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Nestin Antibody

	Immunocytochemistry Nestin in Human Neural Progenitor Cells. Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Intracellular Staining by Flow Cytometry Detection of Nestin in A172 cells by Flow Cytometry. A172 cells were stained with Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # MAB1259, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Nestin

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

Item	Value
References	Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310. Lendahl, U. et al. (1990) Cell 60:585. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144. Tohyama, T. et. al. (1992) Lab. Invest. 66:303. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720. Frederiksen, K. et al. (1988) Neuron 1:439. Cattaneo, E. et al. (1990) Nature 347:762. Reynolds, B.A. and S. Weiss (1992) Science 255:1707. Rietze, R.L. et al. (2001) Nature 412:736. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383. Zulewski, H. et al. (2001) Diabetes 50:521. Lumelsky, N. et al. (2001) Science 292:1389. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670. Shih, C.C. et al. (2001) Blood 98:2412.
Entrez Gene IDs	10763 (Human); 18008 (Mouse); 25491 (Rat)
Alternate Names	FLJ21841, NES, Nestin

Citations for Human Nestin Antibody (99)

Showing 1 - 10 of 99 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Nestin Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

K Kaushal et al. (2022-01-01), Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis Cell Death and Differentiation, 0(0).
PMID: 35273362
Species: Human
Sample Types: Whole Tissue
Applications: IF

B Polat et al. (2022-01-01), Differences in stem cell marker and osteopontin expression in primary and recurrent glioblastoma Cancer Cell International, 22(1):87.
PMID: 35183162
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Schwarz et al. (2022-01-01), Steady-State Levels of Miro1 Linked to Phosphorylation at Serine 156 and Mitochondrial Respiration in Dopaminergic Neurons Cells, 11(8).
PMID: 35455950
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Tisch et al. (2022-01-01), Generation of the human induced pluripotent stem cell line (IBKMOLi002-A) from PBMCs of a patient carrying the heterozygous L271H mutation of the voltage-gated calcium channel subunit Cav1.3-encoding CACNA1D gene Stem Cell Research, 61(0):102784.
PMID: 35453044
Species: Human
Sample Types: Whole Cells
Applications: ICC

B Bohl et al. (2022-01-01), Asymmetric Notch activity by differential inheritance of lysosomes in human neural stem cells Science Advances, 8(6):eabl5792.
PMID: 35148180
Species: Human
Sample Types: Cell Culture Supernates
Applications: ICC

YW Cho et al. (2022-01-01), Single metal-organic framework-embedded nanopit arrays: A new way to control neural stem cell differentiation Science Advances, 8(16):eabj7736.
PMID: 35442746
Species: Human
Sample Types: Whole Cells
Applications: ICC

RR Khadim et al. (2022-01-01), Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs International Journal of Molecular Sciences, 23(13).
PMID: 35806277
Species: Human
Sample Types: Whole Cells
Applications: ICC

YH Chen et al. (2022-01-01), Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1 Oncogene, 63(0):102845.
PMID: 35728441
Species: Human
Sample Types: Whole Tissue
Applications: IHC

L Adnani et al. (2022-01-01), Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells Nature Communications, 13(1):5494.
PMID: 36123372
Species: Human
Sample Types: Organoids
Applications: IHC

J Brás et al. (2022-01-01), Establishment and characterization of human pluripotent stem cells-derived brain organoids to model cerebellar diseases Scientific Reports, 12(1):12513.
PMID: 35869235
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

G Rastoldo et al. (2022-01-01), Vestibular Nuclei: A New Neural Stem Cell Niche? Cells, 11(22).
PMID: 36429025
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

EJ Kim et al. (2022-01-01), The oncogenic JAG1 intracellular domain is a transcriptional cofactor that acts in concert with DDX17/SMAD3/TGIF2 Cell Reports, 41(8):111626.
PMID: 36417870
Species: Human
Sample Types: Whole Cells
Applications: ICC

H Walgrave et al. (2021-01-01), Restoring miR-132 expression rescues adult hippocampal neurogenesis and memory deficits in Alzheimer's disease Cell Stem Cell, 0(0).
PMID: 34033742
Species: Human
Sample Types: Whole Cells
Applications: ICC

F Xiong et al. (2021-01-01), RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability Cell Research, 0(0).
PMID: 34108665
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Rajasingh et al. (2021-01-01), Comparative analysis of human induced pluripotent stem cell-derived mesenchymal stem cells and umbilical cord mesenchymal stem cells Journal of Cellular and Molecular Medicine, 0(0).
PMID: 34390186
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Gil-Ranedo et al. (2021-01-01), Viral targeting of glioblastoma stem cells with patient-specific genetic and post-translational p53 deregulations Cell Reports, 36(10):109673.
PMID: 34496248
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Y Luo et al. (2021-01-01), Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development Stem Cell Research, 57(0):102583.
PMID: 34710837
Species: Human
Sample Types: Whole Tissue
Applications: IHC

PA Barbuti et al. (2021-01-01), Gene-corrected p.A30P SNCA patient-derived isogenic neurons rescue neuronal branching and function Scientific Reports, 11(1):21946.
PMID: 34754035
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

GL Boulting et al. (2021-01-01), Activity-dependent regulome of human GABAergic neurons reveals new patterns of gene regulation and neurological disease heritability Nature Neuroscience, 0(0).
PMID: 33542524
Species: Human
Sample Types: Whole Cells
Applications: IHC

Y Shamshirga et al. (2021-01-01), Rapid target validation in a Cas9-inducible hiPSC derived kidney model Scientific Reports, 11(1):16532.
PMID: 34400685
Species: Human
Sample Types: Whole Cells
Applications: ICC

RB Bressan et al. (2021-01-01), Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants Cell Stem Cell, 0(0).
PMID: 33631116
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MJ Ruiz-Magañ et al. (2021-01-01), Decidualization modulates the mesenchymal stromal/stem cell and pericyte characteristics of human decidual stromal cells. Effects on antigen expression, chemotactic activity on monocytes and antitumoral activity Journal of reproductive immunology, 145(0):103326.
PMID: 33965695
Species: Human

Applications: IF

N Micali et al. (2020-01-01), Variation of Human Neural Stem Cells Generating Organizer States Inï¿½Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates Cell Rep, 31(5):107599.
PMID: 32375049
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SH Oh et al. (2020-01-01), Multimodal Therapeutic Effects of Neural Precursor Cells Derived from Human-Induced Pluripotent Stem Cells through Episomal Plasmid-Based Reprogramming in a Rodent Model of Ischemic Stroke Stem Cells Int, 2020(0):4061516.
PMID: 32269595
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot

H Xu et al. (2020-01-01), FKBP9 promotes the malignant behavior of glioblastoma cells and confers resistance to endoplasmic reticulum stress inducers J. Exp. Clin. Cancer Res., 39(1):44.
PMID: 32111229
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

LR Gauthier et al. (2020-01-01), The HIF1&alpha/JMY pathway promotes glioblastoma stem-like cell invasiveness after irradiation Sci Rep, 10(1):18742.
PMID: 33128011
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Wu et al. (2020-01-01), Generation of a human iPSC line CIBi008-A from amniotic fluid-derived cells of a fetus with &beta-thalassemia carrying variants of -28Aï¿½>ï¿½G and IVS-II-654Cï¿½>ï¿½T in HBB Stem Cell Res, 49(0):102074.
PMID: 33157391
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

IA Ciechomska et al. (2020-01-01), EGFR/FOXO3a/BIM signaling pathway determines chemosensitivity of BMP4-differentiated glioma stem cells to temozolomide Exp. Mol. Med., 0(0).
PMID: 32788653
Species: Human
Sample Types: Whole Cells
Applications: ICC

CC Liang et al. (2020-01-01), Effect of amniotic fluid stem cell transplantation on the recovery of bladder dysfunction in spinal cord-injured rats Sci Rep, 10(1):10030.
PMID: 32572272
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Matoug-Elw et al. (2020-01-01), Ex-vivo recellularisation and stem cell differentiation of a decellularised rat dental pulp matrix Scientific Reports, 10(1):21553.
PMID: 33299073
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Colmenero- et al. (2020-01-01), Identification of VRK1 as a New Neuroblastoma Tumor Progression Marker Regulating Cell Proliferation Cancers (Basel), 12(11).
PMID: 33233777
Species: Human
Sample Types: Whole Cells
Applications: ICC

P Barbuti et al. (2020-01-01), Using High-Content Screening to Generate Single-Cell Gene-Corrected Patient-Derived iPS Clones Reveals Excess Alpha-Synuclein with Familial Parkinson's Disease Point Mutation A30P Cells, 9(9).
PMID: 32927687
Species: Human
Sample Types: Whole Cells
Applications: ICC

DP Collins et al. (2020-01-01), Development of immortalized human hepatocyte-like hybrid cells by fusion of multi-lineage progenitor cells with primary hepatocytes PLoS ONE, 15(6):e0234002.
PMID: 32497071
Species: Human
Sample Types: Tissue Array
Applications: IHC

I Bertolini et al. (2019-01-01), A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes EBioMedicine, 0(0).
PMID: 30737083
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

BW Stringer et al. (2019-01-01), A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma Sci Rep, 9(1):4902.
PMID: 30894629
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Ross et al. (2019-01-01), Characterization of the first induced pluripotent stem cell line generated from a patient with autosomal dominant catecholaminergic polymorphic ventricular tachycardia due to a heterozygous mutation in cardiac calsequestrin-2 Stem Cell Res, 37(0):101450.
PMID: 31039485
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

J Luzuriaga et al. (2019-01-01), Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate Into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain Front Physiol, 10(0):347.
PMID: 30984027
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC

C Zalfa et al. (2019-01-01), Transplantation of clinical-grade human neural stem cells reduces neuroinflammation, prolongs survival and delays disease progression in the SOD1 rats Cell Death Dis, 10(5):345.
PMID: 31024007
Species: Human
Sample Types: Whole Tissue
Applications: IHC

M Huang et al. (2019-01-01), Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis Cell Stem Cell, 0(0).
PMID: 31204176
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Chanda et al. (2019-01-01), Direct Reprogramming of Human Neurons Identifies MARCKSL1 as a Pathogenic Mediator of Valproic Acid-Induced Teratogenicity Cell Stem Cell, 25(1):103-119.e6.
PMID: 31155484
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Marrone et al. (2018-01-01), Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease PLoS ONE, 13(3):e0192497.
PMID: 29513666
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

F Hsu et al. (2018-01-01), Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria Elife, 7(0).
PMID: 29469808
Species: Human
Sample Types: Whole Cells
Applications: ICC

F Wang et al. (2018-01-01), ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways Oncogene, 37(3):286-301.
PMID: 28925404
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

S Moll et al. (2018-01-01), Selective pharmacological inhibition of DDR1 prevents experimentally-induced glomerulonephritis in prevention and therapeutic regime J Transl Med, 16(1):148.
PMID: 29859097
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

R Tirughana et al. (2018-01-01), GMP Production and Scale-Up of Adherent Neural Stem Cells with a Quantum Cell Expansion System Mol Ther Methods Clin Dev, 10(0):48-56.
PMID: 29992178
Species: Human
Sample Types: Whole Tissue
Applications: IHC

J Rosati et al. (2018-01-01), Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies Cell Death Dis, 9(10):937.
PMID: 30224709
Species: Human
Sample Types: Whole Cells
Applications: ICC

CP Beier et al. (2018-01-01), Aberrant neuronal differentiation is common in glioma but is associated neither with epileptic seizures nor with better survival Sci Rep, 8(1):14965.
PMID: 30297697
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Holliday et al. (2018-01-01), Generation of an induced pluripotent stem cell line from a hypertrophic cardiomyopathy patient with a pathogenic myosin binding protein C (MYBPC3) p.Arg502Trp mutation Stem Cell Res, 33(0):56-59.
PMID: 30316040
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Shafa et al. (2018-01-01), Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers Front Med (Lausanne), 5(0):69.
PMID: 29600249
Species: Human
Sample Types: Whole Cells
Applications: ICC

H Yao et al. (2018-01-01), Intracellular pH Regulation in iPSCs-derived Astrocytes from Subjects with Chronic Mountain Sickness Neuroscience, 0(0).
PMID: 29438800
Species: Human
Sample Types: Whole Cells
Applications: ICC

D Poppe et al. (2018-01-01), Genome Editing in Neuroepithelial Stem Cells to Generate Human Neurons with High Adenosine-Releasing Capacity Stem Cells Transl Med, 0(0).
PMID: 29589874
Species: Human
Sample Types: Whole Cells
Applications: ICC

D Ghoubay-Be et al. (2017-01-01), Easy xeno-free and feeder-free method for isolating and growing limbal stromal and epithelial stem cells of the human cornea PLoS ONE, 12(11):e0188398.
PMID: 29149196
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

P Wang et al. (2017-01-01), HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 8(17):28074-28092.
PMID: 28427209
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

HO King et al. (2017-01-01), RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells Stem Cell Reports, 8(1):125-139.
PMID: 28076755
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

JH Lee et al. (2017-01-01), Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency Cell Rep, 19(1):20-35.
PMID: 28380358
Species: Human
Sample Types: Whole Cells
Applications: ICC

SB Ross et al. (2017-01-01), Generation of induced pluripotent stem cells (iPSCs) from a hypertrophic cardiomyopathy patient with the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7) gene Stem Cell Res, 20(0):88-90.
PMID: 28395747
Species: Human
Sample Types: Whole Cells
Applications: ICC

R Kumar et al. (2017-01-01), Induction of senescence in primary glioblastoma cells by serum and TGF? Sci Rep, 7(1):2156.
PMID: 28526854
Species: Human
Sample Types: Whole Cells
Applications: ICC

X Yuan et al. (2017-01-01), A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model PLoS ONE, 12(4):e0174074.
PMID: 28441409
Species: Human
Sample Types: Whole Cells
Applications: IHC-P

AM Rosager et al. (2017-01-01), Transferrin receptor-1 and ferritin heavy and light chains in astrocytic brain tumors: Expression and prognostic value PLoS ONE, 12(8):e0182954.
PMID: 28837569
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

SS Jensen et al. (2017-01-01), Effects of the lysosomal destabilizing drug siramesine on glioblastoma in vitro and in vivo BMC Cancer, 17(1):178.
PMID: 28270132
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

(2016-01-01), Cytosine-5 RNA Methylation Regulates Neural Stem Cell Differentiation andï¿½Motility Stem Cell Reports, 0(0).
PMID: 28041877
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

(2016-01-01), BIX01294, an inhibitor of histone methyltransferase, induces autophagy-dependent differentiation of glioma stem-like cells Sci Rep, 6(0):38723.
PMID: 27934912
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

(2016-01-01), Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype PLoS ONE, 11(5):e0155106.
PMID: 27171431
Species: Human
Sample Types: Whole Cells
Applications: ICC

Florian Wegner (2016-01-01), Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization J. Neurosci., 36(47):12027-12043.
PMID: 27881786
Species: Human
Sample Types: Whole Tissue
Applications: ICC

(2016-01-01), Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors PLoS ONE, 11(12):e0169129.
PMID: 28030635
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

Jeitany M et al. (2015-01-01), A preclinical mouse model of glioma with an alternative mechanism of telomere maintenance (ALT). Int J Cancer, 136(7):1546-58.
PMID: 25175359
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Lee J et al. (2015-01-01), Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells. Stem Cells, 33(4):1142-52.
PMID: 25639500
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Soeda et al. (2015-01-01), The evidence of glioblastoma heterogeneity. Sci Rep, 5(0):7979.
PMID: 25623281
Species: Human
Sample Types: Whole Cells
Applications: ICC

Narita K et al. (2014-01-01), Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma. Int J Oncol, 44(4):1118-30.
PMID: 24481417
Species: Human
Sample Types: Whole Cells
Applications: ICC

Mitchell R et al. (2014-01-01), Molecular evidence for OCT4-induced plasticity in adult human fibroblasts required for direct cell fate conversion to lineage specific progenitors. Stem Cells, 32(8):2178-87.
PMID: 24740884
Species: Human
Sample Types: Whole Cells
Applications: ICC

Jiang et al. (2014-01-01), Novel anti-glioblastoma agents and therapeutic combinations identified from a collection of FDA approved drugs. J Transl Med, 12(0):13.
PMID: 24433351
Species: Human
Sample Types: Whole Cells
Applications: ICC

Sauder C et al. (2014-01-01), Phenotypic plasticity in normal breast derived epithelial cells. BMC Cell Biol, 15(0):20.
PMID: 24915897
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

Fiaschetti G et al. (2014-01-01), Epigenetic silencing of miRNA-9 is associated with HES1 oncogenic activity and poor prognosis of medulloblastoma. Br J Cancer, 110(3):636-47.
PMID: 24346283
Species: Human
Sample Types: Whole Cells
Applications: ICC

Mertens J et al. (2013-01-01), Embryonic stem cell-based modeling of tau pathology in human neurons. Am J Pathol, 182(5):1769-79.
PMID: 23499461
Reinhardt P et al. (2013-01-01), Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling. PLoS ONE, 8(3):e59252.
PMID: 23533608
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Akiyama et al. (2013-01-01), Nestin is highly expressed in advanced-stage melanomas and neurotized nevi. Oncol Rep, 29(4):1595-9.
PMID: 23417116
Species: Human
Sample Types: Whole Cells
Applications: ICC

Farmer et al. (2013-01-01), Activation of the type I interferon pathway is enhanced in response to human neuronal differentiation. PLoS ONE, 8(3):e58813.
PMID: 23505563
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Ricciardi M et al. (2012-01-01), Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow. PLoS ONE, 7(5):e35639.
PMID: 22567106
Species: Human
Sample Types: Whole Cells
Applications: ICC

Emdad L et al. (2012-01-01), Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes. Stem Cells Dev., 21(3):404-10.
PMID: 21631388
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Fusi A et al. (2011-01-01), Expression of the stem cell markers nestin and CD133 on circulating melanoma cells. J. Invest. Dermatol., 131(2):487-94.
PMID: 20882037
Species: Human
Sample Types: Whole Cells
Applications: ICC

Engelen E et al. (2011-01-01), Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes. Nat. Genet., 43(6):607-11.
PMID: 21532573
Species: Human
Sample Types: Whole Cells
Applications: ICC

Gil JE et al. (2009-01-01), Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett., 583(3):561-7.
PMID: 19162023
Species: Human
Sample Types: Whole Cells
Applications: ICC

Lamba S et al. (2009-01-01), Glial cell type-specific subcellular localization of 14-3-3 zeta: an implication for JCV tropism. Glia, 57(9):971-7.
PMID: 19062179
Species: Human
Sample Types: Whole Cells
Applications: ICC

Yuki K et al. (2009-01-01), Induction of oligodendrogenesis in glioblastoma-initiating cells by IFN-mediated activation of STAT3 signaling. Cancer Lett., 284(1):71-9.
PMID: 19457609
Species: Human
Sample Types: Whole Cells
Applications: ICC

Ladewig J et al. (2008-01-01), Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons. Stem Cells, 26(7):1705-12.
PMID: 18420830
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Vrana NE et al. (2008-01-01), Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures. Invest. Ophthalmol. Vis. Sci., 49(12):5325-31.
PMID: 18708614
Species: Human
Sample Types: Whole Cells
Applications: ICC

Sun Y et al. (2008-01-01), Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Mol. Cell. Neurosci., 38(2):245-58.
PMID: 18450476
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Hattermann K et al. (2008-01-01), The chemokine CXCL16 induces migration and invasion of glial precursor cells via its receptor CXCR6. Mol. Cell. Neurosci., 39(1):133-41.
PMID: 18619850
Species: Human
Sample Types: Whole Cells
Applications: ICC

Ishii J et al. (2007-01-01), The free-radical scavenger edaravone restores the differentiation of human neural precursor cells after radiation-induced oxidative stress. Neurosci. Lett., 423(3):225-30.
PMID: 17709197
Species: Human
Sample Types: Whole Cells
Applications: ICC

Shin S et al. (2007-01-01), Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue. Stem Cells, 25(5):1298-306.
PMID: 17272497
Species: Human
Sample Types: Whole Cells
Applications: ICC

Neuhoff S et al. (2007-01-01), Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro. Exp. Hematol., 35(7):1119-31.
PMID: 17588481
Species: Human
Sample Types: Whole Cells
Applications: ICC

Inagaki A et al. (2007-01-01), Long-term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions. Biochem. Biophys. Res. Commun., 361(3):586-92.
PMID: 17673180
Species: Human
Sample Types: Whole Cells
Applications: ICC

Kim BK et al. (2006-01-01), Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells. FEBS Lett., 580(25):5869-74.
PMID: 17027979
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Porat Y et al. (2006-01-01), Isolation of an adult blood-derived progenitor cell population capable of differentiation into angiogenic, myocardial and neural lineages. Br. J. Haematol., 135(5):703-14.
PMID: 17052254
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Sheen VL et al. (2006-01-01), Impaired proliferation and migration in human Miller-Dieker neural precursors. Ann. Neurol., 60(1):137-44.
PMID: 16642511
Species: Human
Sample Types: Whole Cells
Applications: ICC

Shin S et al. (2006-01-01), Long-term proliferation of human embryonic stem cell-derived neuroepithelial cells using defined adherent culture conditions. Stem Cells, 24(1):125-38.
PMID: 16100006
Species: Human
Sample Types: Whole Cells
Applications: ICC

Segev H et al. (2004-01-01), Differentiation of human embryonic stem cells into insulin-producing clusters. Stem Cells, 22(3):265-74.
PMID: 15153604
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ni HT et al. (2004-01-01), High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells. Brain Res. Dev. Brain Res., 152(2):159-69.
PMID: 15351504
Species: Human
Sample Types: Whole Cells
Applications: ICC

Gerecht-Nir S et al. (2004-01-01), Three-dimensional porous alginate scaffolds provide a conducive environment for generation of well-vascularized embryoid bodies from human embryonic stem cells. Biotechnol. Bioeng., 88(3):313-20.
PMID: 15486935

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Human Nestin Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Neuroblastoma cells

Species: Human

Verified Customer | Posted 06/17/2022
Human Nestin Antibody

Name: Anonymous

Application: WB

Sample Tested: Cell Lysates

Species: Human

Verified Customer | Posted 10/26/2015

Specificity: Reasonably specificSensitivity: Reasonably sensitiveBuffer: 5% non-fat milk in TBSTDilution: 1/1000
Human Nestin Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Human cell line

Species: Human

Verified Customer | Posted 10/26/2015

Specificity: SpecificSensitivity: Reasonably sensitiveBuffer: 1% BSA + 0.3% Triton X-100 in PBSDilution: 1/100
Human Nestin Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: HeLa cells

Species: Human

Verified Customer | Posted 10/26/2015

Specificity: Reasonably specificSensitivity: Reasonably sensitiveBuffer: 1% BSA + 0.1% Triton X-100 in PBSDilution: 1/100

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