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ITS Media Supplement (100X)

R&D Systems, Inc. a Bio-Techne Brand

Insulin-Transferrin-Selenium Media Supplement

Key Product Details

ITS Media Supplement Supports Proliferation Under Low Serum Conditions.
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AR013
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, ITS Media Supplement is prepared using the following procedure:

  • Dilute the media into the cell culture media of interest
  • Store completed media at 2 – 8 °C
 

 

Reagents Provided

Reagents provided in the ITS Media Supplement (100X) (Catalog # AR013):

Component Amount (µg/mL)
Recombinant Human Insulin 1000
Human Transferrin 500
Selenite 0.67

 

Stability and Storage

Store in the dark at < -20 °C in a manual defrost freezer. Do not use past the expiration date.

Precautions

This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious reagents, these reagents should be handled as if capable of transmitting infection.

 

Other Supplies Required

Materials

  • Basal media
  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 2 °C to 8 °C refrigerator

 

Procedure Overview

Dilute 100-fold into cell culture media of interest before use. The final concentration of serum required with ITS supplementation is cell type and assay dependent. Titrate for individual use.

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Customer Reviews for ITS Media Supplement (100X) (5)

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  • ITS Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 01/13/2020
  • ITS Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 10/02/2019
  • ITS Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 09/30/2019
  • ITS Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 06/20/2019
  • ITS Media Supplement (100X)
    Name: Leslie Priddy
    Verified Customer | Posted 04/04/2018

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Product Documents for ITS Media Supplement (100X)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.