Recombinant Human M-CSF Biotinylated Protein, CF

Name: Recombinant Human M-CSF Biotinylated Protein, CF
Brand: R&D Systems
SKU: BT216

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BT216

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Biotinylated

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Key Product Details

Source:	E. coli
Accession #:	NP_757350.2
Structure / Form:	Disulfide-linked homodimer Biotinylated via amines
Applications:	Bioactivity

View Full Technical Details for Recombinant Human M-CSF Biotinylated Protein, CF

Product Specifications for Recombinant Human M-CSF Biotinylated Protein, CF

Source	E. coli-derived human M-CSF protein Glu33-Ser190, with an N-terminal Met
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	18.5 kDa
SDS-PAGE	15-22 kDa, under reducing conditions.
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human M-CSF R/CD115 Fc Chimera Protein (Catalog # 329-MR) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of &Biotinylated Recombinant Human M‑CSF (Catalog # BT216) that produces 50% of the optimal binding response is 1.50‑15.0 ng/mL.

Scientific Data Examples for Recombinant Human M-CSF Biotinylated Protein, CF

	Biotinylated Recombinant Human M‑CSF Protein Binding Activity. When Recombinant Human M-CSF R/CD115 Fc Chimera Protein (329-MR) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human M‑CSF Protein (Catalog # BT216) that produces 50% of the optimal binding response is 1.50-15.0 ng/mL.
	Biotinylated Recombinant Human M‑CSF Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human M‑CSF Protein (Catalog # BT216) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 15-22 kDa and 30-40 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT216

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode
M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N‑terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M‑CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Item	Value
References	Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125. Ryan, G.R. et al. (2001) Blood 98:74. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178. Nandi, S. et al. (2006) Blood 107:786. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035. Koths, K. (1997) Mol. Reprod. Dev. 46:31. Jang, M-H. et al. (2006) J. Immunol. 177:4055. Kawasaki, E.S. et al. (1985) Science 230: 291. Wong, G.G. et al. (1987) Science 235:1504.
Long Name	Macrophage Colony Stimulating Factor
Entrez Gene IDs	1435 (Human); 12977 (Mouse)
Alternate Names	CSF-1, CSF1, Lanimostim, M-CSF, MCSF, MCSFlanimostim, MGC31930, colony stimulating factor 1 (macrophage), macrophage colony stimulating factor, macrophage colony-stimulating factor 1

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Recombinant Human M-CSF Biotinylated Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human M-CSF Biotinylated Protein, CF

Scientific Data Examples for Recombinant Human M-CSF Biotinylated Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: M-CSF

Citations for Recombinant Human M-CSF Biotinylated Protein, CF

Product Documents for Recombinant Human M-CSF Biotinylated Protein, CF

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