Recombinant Human M-CSF R/CD115 Fc Chimera Protein

Name: Recombinant Human M-CSF R/CD115 Fc Chimera Protein
Brand: R&D Systems
SKU: 329-MR
Price: 428 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 329-MR

Citations (6)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	CAA27300
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human M-CSF R/CD115 Fc Chimera Protein

Product Specifications for Recombinant Human M-CSF R/CD115 Fc Chimera Protein

Source

Mouse myeloma cell line, NS0-derived human M-CSF R/CD115 protein

Human M-CSF R (Ile20-Glu512) Accession # CAA27300	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ile20

Predicted Molecular Mass

81 kDa (monomer)

SDS-PAGE

150 kDa, reducing conditions

Activity

Measured by its ability to inhibit the M-CSF-induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Halenbeck, R. et al. (1989) Biotechnology 7:710. The ED₅₀ for this effect is 0.004-0.012 µg/mL in the presence of 1 ng/mL of Recombinant Human M-CSF (Catalog # 216-MC).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

329-MR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

329-MR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: M-CSF R/CD115

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin alpha _v beta ₃ share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased
M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

Item	Value
References	deParseval, N. et al. (1993) Nucleic Acids Res. 21:750. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88. Rovida, E. et al. (2001) J. Immunol. 166:1583. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128. Dai, X. et al. (2002) Blood 99:111. Faccio, R. et al. (2003) J. Clin. Invest. 111:749. Li, M. et al. (2004) J. Neurochem. 91:623. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.
Long Name	Macrophage Colony Stimulating Factor Receptor
Entrez Gene IDs	1436 (Human); 12978 (Mouse)
Alternate Names	CD115, CD115 antigen, CSF-1-R, CSF1R, CSFR, EC 2.7.10.1, FMS proto-oncogene, FMSFIM2, M-CSF R, M-CSFR, MCSFR, McDonough feline sarcoma viral (v-fms) oncogene homolog, Proto-oncogene c-Fms, c-fms, colony stimulating factor 1 receptor, macrophage colony stimulating factor I receptor, macrophage colony-stimulating factor 1 receptor

Citations for Recombinant Human M-CSF R/CD115 Fc Chimera Protein (6)

Showing 1 - 6 of 6 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human M-CSF R/CD115 Fc Chimera Protein include:

Species: Primate
Sample Types: Whole Cells
Applications: Bioassay

(2016), Enhanced antagonism of BST-2 by a neurovirulent SIV envelope J Clin Invest, 0(0).
PMID: 27159392
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), The nuclear receptor LXR modulates interleukin-18 levels in macrophages through multiple mechanisms Sci Rep, 6(0):25481.
PMID: 27149934
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Effects of Cigarette Smoke Condensate on Oxidative Stress, Apoptotic Cell Death, and HIV Replication in Human Monocytic Cells PLoS ONE, 11(5):e0155791.
PMID: 27203850
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JE Ussher et al. (2016), TLR signalling in human antigen-presenting cells regulates MR1-dependent activation of MAIT cells Eur J Immunol, 0(0).
PMID: 27105778
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AS Haka et al. (2016), Exocytosis of Macrophage Lysosomes Leads to Digestion of Apoptotic Adipocytes and Foam Cell Formation J Lipid Res, 0(0).
PMID: 27044658
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lamprecht B et al. (2010), Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma. Nat. Med., 16(5):571-9.
PMID: 20436485

Recombinant Human M-CSF R/CD115 Fc Chimera Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Human M-CSF R/CD115 Fc Chimera Protein

Formulation, Preparation and Storage

Carrier Free

329-MR

Carrier

329-MR/CF

Carrier Free

Reconstitution Calculator

Background: M-CSF R/CD115

Citations for Recombinant Human M-CSF R/CD115 Fc Chimera Protein (6)

Product Documents for Recombinant Human M-CSF R/CD115 Fc Chimera Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected