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Recombinant Human CEACAM-5/CD66e Fc Chimera Protein, CF

Catalog # 10449-CM | R&D Systems, Inc. a Bio-Techne Brand
HEK293 Expressed Protein
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10449-CM-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CEACAM-5/CD66e protein
Human CEACAM-5/CD66e
(Lys35-Ala685)
Accession # NP_004354.3
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys35

Predicted Molecular Mass

96 kDa

SDS-PAGE

135-175 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CEACAM-5/CD66e Fc Chimera (Catalog # 10449-CM) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Galectin-4  (Catalog # 1227-GA) binds with an ED50 of 10-90 ng/mL.

Scientific Data Images for Recombinant Human CEACAM-5/CD66e Fc Chimera Protein, CF

Recombinant Human CEACAM-5/CD66e Fc Chimera Protein Binding Activity

Recombinant Human CEACAM-5/CD66e Fc Chimera Protein Binding Activity

When Recombinant Human CEACAM-5/CD66e Fc Chimera (10449-CM) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Galectin-4 (1227-GA) binds with an ED50 of 10-90 ng/mL.
Recombinant Human CEACAM-5/CD66e Fc Chimera Protein SDS-PAGE

Recombinant Human CEACAM-5/CD66e Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CEACAM-5/CD66e Fc Chimera Protein (Catalog # 10449-CM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 135-175 kDa and 270-330 kDa, respectively.

Formulation, Preparation and Storage

10449-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA, CD66e and Psg30, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM family members are highly glycosylated with varying arrangements of IgV-like and IgC-like regions in their extracellular domains (ECD) and can be expressed as transmembrane, glycophosphatidyl inositol (GPI) linked or soluble proteins (1-3).  CEACAM-5 consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2, 4, 5). While the mature ECD of human CEACAM-5 shares 26% amino acid identity with mouse CEACAM-5, it remains unclear if these molecules are direct orthologs (6). CEACAM-5, expressed primarily by epithelial cells, functions as a calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (1, 7). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (8). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is up-regulated in a wide variety of human tumors, promoting tumor cell migration, invasion, adhesion, and metastasis, and has been used as a cancer marker (8, 9). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (10, 11). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (12). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (8). CEACAM-5 was shown to bind galectin-4 and by surface plasmon resonance and coimmunoprecipitated with galectin-4 in human colon adenocarcinoma LS174T cell lysates  (13). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14, 15). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References

  1. Zebhauser, R. et al. (2005) Genomics 86:566.
  2. Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
  3. Chan, et al. (2007) Curr Oncol. 14:70.
  4. Schrewe, H. et al. (1990) Mol. Cell. Biol. 10:2738.
  5. Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648.
  6. Hance, K.W. et al. Mutat Res. (2005) 576:132.
  7. Stern, N. et al. (2005) J. Immunol. 174:6692.
  8. Beauchemin N. et al. (2013) Cancer Metastasis Rev. 32:3.
  9. Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
  10. Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
  11. Ordonez, C. et al. (2000) Cancer Res. 60:3419.
  12. Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
  13. Ideo, H. et al. (2005) J Biol Chem. 280:4730.
  14. Martin, J.N. et al. (2016) Biochemistry. 55:4286.
  15. Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.
  16. Tchoupa, A.K. et al. (2014) Cell Commun Signal. 12:27.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Alternate Names

CD66e, CEA, CEACAM5

Entrez Gene IDs

1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)

Gene Symbol

CEACAM5

UniProt

Product Documents for Recombinant Human CEACAM-5/CD66e Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CEACAM-5/CD66e Fc Chimera Protein, CF

For research use only

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