Recombinant Human Galectin-4 Protein

Name: Recombinant Human Galectin-4 Protein
Brand: R&D Systems
SKU: 1227-GA
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1227-GA

Citations (3)

(1)

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Key Product Details

Source:	E. coli
Accession #:	P56470
Applications:	Binding Activity, Bioactivity

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Product Specifications for Recombinant Human Galectin-4 Protein

Source	E. coli-derived human Galectin-4 protein Ala2-Ile323
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala2
Predicted Molecular Mass	36 kDa
Activity	Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED₅₀ for this effect is 2-10 µg/mL. Measured by its binding activity in a functional ELISA. Ideo, H. et al. (2005) J. Biol. Chem. 280:4730. Recominant Human Galectin-4 can bind Recombinant Human CEACAM‑5/CD66e (Catalog # 4128-CM) with an estimated K_d <4 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1227-GA

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1227-GA/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-4

Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups often termed prototype (one CRD), tandem-repeat (two CRDs) and chimeric (one CRD, unique N-terminus) (1, 2). All lack classical signal peptides, but are present and active both within and outside of the cell. Galectins are involved in cell adhesion, migration, survival and apoptosis, and are often up- or down-regulated in cancer (1 - 3). Galectin-4 is a 36 kDa tandem-repeat galectin found throughout the gastrointestinal tract, but also present in well-differentiated breast and liver carcinomas (3, 4). Each CRD binds a different set of carbohydrate groups, including those found on red cell blood group antigens (3, 5). CRD1 also binds cholesterol 3-sulfate and other sulfatides, which are concentrated within lipid raft membrane microdomains (6, 7). Endocytosed Galectin-4 is thought to play a role in forming the rafts, delivering them to the intestinal apical membrane, and stabilizing highly detergent-resistant "superrafts" (7, 9). Human Galectin-4 shares 76%, 77%, 78% and 80% aa identity with mouse, rat, bovine and porcine Galectin-4, respectively, with the highest identity occurring within the CRDs. A potential splice variant begins at aa 132 and lacks most of the first CRD (10). Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation (11, 12). Either pro- or anti‑inflammatory activity has been shown, depending on the mouse model used. Galectin-4 can also bind lung, spleen and kidney macrophages, although its expression is normally low in these tissues (5).

Item	Value
References	Yang, R-Y. et al. (2008) Expert Rev. Mol. Med. 10:e17. Elola, M. T. et al. (2007) Cell. Mol. Life Sci. 64:1679. Huflejt, M. E. & H. Leffler (2004) Glycoconj. J. 20:247. Recreche, H. et al. (1997) Eur. J. Biochem. 248:225. Markova, V. et al. (2006) Int. J. Mol. Med. 18:65. Ideo, H. et al. (2007) J. Biol. Chem. 282:21081. Delacour, D. et al. (2005) J. Cell Biol. 169:491. Braccia, A. et al. (2003) J. Biol. Chem. 278:15679. Stechly, L. et al. (2009) Traffic Jan 24 e-pub ahead of print. Entrez Accession # EAW56820. Hokama, A. et al. (2004) Immunity 20:681. Paclik, D. et al. (2008) PloS ONE 3:e2629.
Entrez Gene IDs	3960 (Human); 16855 (Mouse)
Alternate Names	Antigen NY-CO-27, GAL4, Galectin-4, Galectin4, L-36 lactose-binding protein, L36LBP, LGALS4, Lactose-binding lectin 4, gal-4, galectin 4, lectin, galactoside-binding, soluble, 4

Citations for Recombinant Human Galectin-4 Protein (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Galectin-4 Protein include:

Species: Human
Sample Types: Natural Protein
Applications: Bioassay

J Terävä et al. (2019), Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma PLoS ONE, 14(7):e0219480.
PMID: 31344060
Species: Human
Sample Types: Protein
Applications: Bioassay

Madireddi S et al. (2014), Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies. J Exp Med, 211(7):1433-48.
PMID: 24958847
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay

Paclik D et al. (2008), Galectin-4 controls intestinal inflammation by selective regulation of peripheral and mucosal T cell apoptosis and cell cycle. PLoS ONE, 3(7):e2629.
PMID: 18612433

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Recombinant Human Galectin-4 Protein

Name: Anonymous

Verified Customer | Posted 10/26/2015

Galectin-4 was used as a positive control on western and looks good.

Recombinant Human Galectin-4 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Galectin-4 Protein

Formulation, Preparation and Storage

Carrier Free

1227-GA

Carrier

1227-GA/CF

Carrier Free

Reconstitution Calculator

Background: Galectin-4

Citations for Recombinant Human Galectin-4 Protein (3)

Customer Reviews for Recombinant Human Galectin-4 Protein (1)

Customer Images

Recombinant Human Galectin-4 Protein

Product Documents for Recombinant Human Galectin-4 Protein

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