Skip to main content

Recombinant Human Galectin-4 Protein

Bio-Techne includes R&D Systems | Catalog # 1227-GA

Catalog #
Availability
Size / Price
Qty
Loading...
Carrier Free
1227-GA-050/CF

Catalog #
Availability
Size / Price
Qty
With Carrier
1227-GA-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Binding Activity, Bioactivity

Product Specifications

Source

E. coli-derived human Galectin-4 protein
Ala2-Ile323

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

36 kDa

Activity

Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385.
The ED50 for this effect is 2-10 µg/mL.

Measured by its binding activity in a functional ELISA. Ideo, H. et al. (2005) J. Biol. Chem. 280:4730.
Recominant Human Galectin-4 can bind Recombinant Human CEACAM‑5/CD66e (Catalog # 4128-CM) with an estimated Kd <4 nM.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1227-GA
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1227-GA/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-4

Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups often termed prototype (one CRD), tandem-repeat (two CRDs) and chimeric (one CRD, unique N-terminus) (1, 2). All lack classical signal peptides, but are present and active both within and outside of the cell. Galectins are involved in cell adhesion, migration, survival and apoptosis, and are often up- or down-regulated in cancer (1 - 3). Galectin-4 is a 36 kDa tandem-repeat galectin found throughout the gastrointestinal tract, but also present in well-differentiated breast and liver carcinomas (3, 4). Each CRD binds a different set of carbohydrate groups, including those found on red cell blood group antigens (3, 5). CRD1 also binds cholesterol 3-sulfate and other sulfatides, which are concentrated within lipid raft membrane microdomains (6, 7). Endocytosed Galectin-4 is thought to play a role in forming the rafts, delivering them to the intestinal apical membrane, and stabilizing highly detergent-resistant "superrafts" (7, 9). Human Galectin-4 shares 76%, 77%, 78% and 80% aa identity with mouse, rat, bovine and porcine Galectin-4, respectively, with the highest identity occurring within the CRDs. A potential splice variant begins at aa 132 and lacks most of the first CRD (10). Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation (11, 12). Either pro- or anti‑inflammatory activity has been shown, depending on the mouse model used. Galectin-4 can also bind lung, spleen and kidney macrophages, although its expression is normally low in these tissues (5).

 

References

  1. Yang, R-Y. et al. (2008) Expert Rev. Mol. Med. 10:e17.
  2. Elola, M. T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
  3. Huflejt, M. E. & H. Leffler (2004) Glycoconj. J. 20:247.
  4. Recreche, H. et al. (1997) Eur. J. Biochem. 248:225.
  5. Markova, V. et al. (2006) Int. J. Mol. Med. 18:65.
  6. Ideo, H. et al. (2007) J. Biol. Chem. 282:21081.
  7. Delacour, D. et al. (2005) J. Cell Biol. 169:491.
  8. Braccia, A. et al. (2003) J. Biol. Chem. 278:15679.
  9. Stechly, L. et al. (2009) Traffic Jan 24 e-pub ahead of print.
  10. Entrez Accession # EAW56820.
  11. Hokama, A. et al. (2004) Immunity 20:681.
  12. Paclik, D. et al. (2008) PloS ONE 3:e2629.

Alternate Names

GAL4, Galectin4, LGALS4

Entrez Gene IDs

3960 (Human); 16855 (Mouse)

Gene Symbol

LGALS4

UniProt

Additional Galectin-4 Products

Product Documents for Recombinant Human Galectin-4 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Galectin-4 Protein

For research use only

Loading...
Loading...
Loading...