This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

Name: Recombinant Human Angiopoietin-like 7 Protein
Brand: R&D Systems
SKU: 914-AN
Rating: 5 (1 reviews)

The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Recombinant Human Angiopoietin-like 7 Protein

Catalog # 914-AN | R&D Systems, Inc. a Bio-Techne Brand

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Citations (2)

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Key Product Details

Source:	CHO
Accession #:	O43827
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Angiopoietin-like 7 Protein

Product Specifications for Recombinant Human Angiopoietin-like 7 Protein

Source	Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 7/ANGPTL7 protein Met1-Pro346, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained: Gln27 predicted
Predicted Molecular Mass	38.6 kDa
SDS-PAGE	49-55 kDa, reducing conditions
Activity	Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED₅₀ for this effect is 50-200 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

914-AN

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

914-AN/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 7/ANGPTL7

Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1 - 3). ANGPTL7 shares 89% aa sequence identity with mouse and rat ANGPTL7. It is secreted as a 45 ‑ 50 kDa monomer that forms disulfide-linked homotrimers and tetramers via the coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor (3, 5). Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5 - 7). Overexpression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans (8). When overexpressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells (9).

Item	Value
References	Oike, Y. et al. (2009) Circ. J. 73:2192. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70. Peek, R. et al. (1998) Invest. Opthalmol. Visual Sci. 39:1782. Peek, R. et al. (2002) J. Biol. Chem. 277:686. Kuchtey, J. et al. (2008) Invest. Opthalmol. Visual Sci. 49:3438. Nehme, A. et al. (2009) BMC Med. Genomics 2:58. Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489. Comes, N. et al. (2010) Genes Cells 16:243. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
Long Name	Angiopoietin-like Protein 7
Entrez Gene IDs	10218 (Human); 654812 (Mouse); 102127960 (Cynomolgus Monkey); 714204 (Rhesus Macaque)
Alternate Names	ANGPTL7, AngX, Angiopoietin-like Protein 7, Angiopoietin-like factor, CDT6, CDT6angiopoietin-like factor (CDT6), Cornea-derived transcript 6 protein, RP4-647M16.2, angiopoietin-like 7, angiopoietin-related protein 7, dJ647M16.1

Citations for Recombinant Human Angiopoietin-like 7 Protein (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Angiopoietin-like 7 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Obenauf A et al. (2015-03-25), Therapy-induced tumour secretomes promote resistance and tumour progression. Nature, 2015-0520(7547):368-72.
PMID: 25807485
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Toyono et al. (2015-01-26), Angiopoietin-like 7 is an anti-angiogenic protein required to prevent vascularization of the cornea. PLoS ONE, 2015-010(1):e0116838.
PMID: 25622036

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Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 07/09/2021

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FAQs for Recombinant Human Angiopoietin-like 7 Protein

This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Recombinant Human Angiopoietin-like 7 Protein

Catalog # 914-AN | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Angiopoietin-like 7 Protein

Formulation, Preparation and Storage

Carrier Free

914-AN

Carrier

914-AN/CF

Carrier Free

Reconstitution Calculator

Background: Angiopoietin-like Protein 7/ANGPTL7

Citations for Recombinant Human Angiopoietin-like 7 Protein (2)

Customer Reviews for Recombinant Human Angiopoietin-like 7 Protein (1)

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Recombinant Human Angiopoietin-like 7 Protein

FAQs for Recombinant Human Angiopoietin-like 7 Protein

Product Documents for Recombinant Human Angiopoietin-like 7 Protein

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