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Recombinant Cynomolgus/Rhesus Neprilysin/CD10 His Protein CF

Bio-Techne includes R&D Systems | Catalog # 10834-ZN

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10834-ZN-020

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived Neprilysin/CD10 protein
Tyr52-Trp750
with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

81 kDa

SDS-PAGE

86-95 kDa, under reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >1200 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Cynomolgus/Rhesus Neprilysin/CD10 His Protein CF

Recombinant Cynomolgus Monkey/Rhesus Macaque Neprilysin/CD10 His-tag Protein Enzyme Activity

Recombinant Cynomologous Monkey/Rhesus Macaque Neprilysin His-tag Protein (Catalog # 10834-ZN) is measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (ES005).

Recombinant Cynomolgus Monkey/Rhesus Macaque Neprilysin/CD10 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque Neprilysin/CD10 His-tag Protein (Catalog # 10834-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-95 kDa.

Formulation, Preparation and Storage

10834-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Neprilysin/CD10

Neprilysin/CD10, also known as NEP, neutral endopeptidase, enkephalinase, and common acute lymphocytic leukemia antigen (CALLA), is a zinc-dependent metallopeptidase type II integral membrane protein of the M13 subfamily of neutral endopeptidases. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. It cleaves and degrades a variety of bioactive peptides including natriuretic peptides, enkephalins, and tachykinins at the amino side of hydrophobic residues. Neprilysin is widely distributed in tissues and highly conserved among mammals (1). Neprilysin is a noncovalently associated homodimer where the monomer is composed of a short intracellular domain, transmembrane helix, and large C-terminal extracellular domain including two domains that form a central cavity containing the active site (2). Although a membrane protein, an alternatively processed soluble form has been documented in circulation and retains similar enzymatic activity to the membrane bound Neprilysin (3). The soluble form is of interest as a prognostic biomarker for heart failure (4,5) and inhibition of Neprilysin is a therapeutic strategy for heart failure (6). Neprilysin was found to be a major degrading enzyme of amyloid beta peptide (A beta) in the brain, making it an important target in Alzheimer's disease studies (7). It metabolizes sensory and inflammatory neuropeptides making it a target for pain perception (8) and is an important marker for some types of lymphomas (9,10). There is also interest in Neprilysin as a biomarker (11) and as a therapeutic inhibition target for treatment of type 2 diabetes (12). 

References

  1. Chen, Y. et al. (2017) Clin. Chem. 63:108.
  2. Oefner, C. et al. (2000) J. Mol. Biol. 296:341.
  3. Aviv, R. et al. (1995) Kidney Int. 47:855.
  4. Bayes-Genis, A. et al. (2015) J. Am. Coll. Cardiol. 65:657. 
  5. Goliasch, G. et al. (2016) Eur. J. Heart Fail. 18:89. 
  6. Von Lueder, T.G. et al. (2013) Circ. Heart Fail. 6:594.
  7. Iwata, N. et al. (2001) Science 292:1550.
  8. Turner, A.J. et al. (2007) Int. Rev. Neurobiol. 82:113. 
  9. De Jong, D. et al. (2007) J. Clin. Oncol. 25:805.
  10. Yang, X.L. et al. (2017) Chin. Med. J. 130:1973.
  11. Gutta, S. et al. (2018) Am. J. Physiol. Renal. Physiol. 315:F263.
  12. Esser, N. et al. (2019) Diabetologia 62:1113.

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Entrez Gene IDs

4311 (Human); 17380 (Mouse); 24590 (Rat); 101925772 (Cynomolgus Monkey)

Gene Symbol

MME

UniProt

Additional Neprilysin/CD10 Products

Product Documents for Recombinant Cynomolgus/Rhesus Neprilysin/CD10 His Protein CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus/Rhesus Neprilysin/CD10 His Protein CF

For research use only

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