Human embryonic kidney cell, HEK293-derived Neprilysin/CD10 protein Tyr52-Trp750 with an N-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
86-95 kDa, under reducing conditions
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005). The specific activity is >1200 pmol/min/μg, as measured under the described conditions.
Recombinant Cynomolgus/Rhesus Neprilysin/CD10 His Protein CF Scientific Data Examples
Recombinant Cynomolgus Monkey/Rhesus Macaque Neprilysin/CD10 His-tag Protein Enzyme Activity
Recombinant Cynomologous Monkey/Rhesus Macaque Neprilysin His-tag Protein (Catalog # 10834-ZN) is measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (ES005).
Recombinant Cynomolgus Monkey/Rhesus Macaque Neprilysin/CD10 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque Neprilysin/CD10 His-tag Protein (Catalog # 10834-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-95 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Neprilysin/CD10, also known as NEP, neutral endopeptidase, enkephalinase, and common acute lymphocytic leukemia antigen (CALLA), is a zinc-dependent metallopeptidase type II integral membrane protein of the M13 subfamily of neutral endopeptidases. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. It cleaves and degrades a variety of bioactive peptides including natriuretic peptides, enkephalins, and tachykinins at the amino side of hydrophobic residues. Neprilysin is widely distributed in tissues and highly conserved among mammals (1). Neprilysin is a noncovalently associated homodimer where the monomer is composed of a short intracellular domain, transmembrane helix, and large C-terminal extracellular domain including two domains that form a central cavity containing the active site (2). Although a membrane protein, an alternatively processed soluble form has been documented in circulation and retains similar enzymatic activity to the membrane bound Neprilysin (3). The soluble form is of interest as a prognostic biomarker for heart failure (4,5) and inhibition of Neprilysin is a therapeutic strategy for heart failure (6). Neprilysin was found to be a major degrading enzyme of amyloid beta peptide (A beta) in the brain, making it an important target in Alzheimer's disease studies (7). It metabolizes sensory and inflammatory neuropeptides making it a target for pain perception (8) and is an important marker for some types of lymphomas (9,10). There is also interest in Neprilysin as a biomarker (11) and as a therapeutic inhibition target for treatment of type 2 diabetes (12).
Chen, Y. et al. (2017) Clin. Chem. 63:108.
Oefner, C. et al. (2000) J. Mol. Biol. 296:341.
Aviv, R. et al. (1995) Kidney Int. 47:855.
Bayes-Genis, A. et al. (2015) J. Am. Coll. Cardiol. 65:657.
Goliasch, G. et al. (2016) Eur. J. Heart Fail. 18:89.
Von Lueder, T.G. et al. (2013) Circ. Heart Fail. 6:594.
Iwata, N. et al. (2001) Science 292:1550.
Turner, A.J. et al. (2007) Int. Rev. Neurobiol. 82:113.
De Jong, D. et al. (2007) J. Clin. Oncol. 25:805.
Yang, X.L. et al. (2017) Chin. Med. J. 130:1973.
Gutta, S. et al. (2018) Am. J. Physiol. Renal. Physiol. 315:F263.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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