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Recombinant Canine Tie-2 Fc Chimera Protein, CF

Catalog # 10720-T2 | R&D Systems, Inc. a Bio-Techne Brand
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10720-T2-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived canine Tie-2 protein
Canine Tie-2
(Ala23-Lys746)
Accession # XP_005626754.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23

Predicted Molecular Mass

107 kDa

SDS-PAGE

115-135 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Canine Tie-2 Fc Chimera (Catalog # 10720-T2) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2  (Catalog # 623-AN) binds with an ED50 of 0.40-3.60 ng/mL.

Scientific Data Images for Recombinant Canine Tie-2 Fc Chimera Protein, CF

Recombinant Canine Tie-2 Fc Chimera Protein Binding Activity.

When Recombinant Canine Tie-2 Fc Chimera (Catalog # 10720-T2) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 (623-AN) binds with an ED50 of 0.40-3.60 ng/mL.

Recombinant Canine Tie-2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Canine Tie-2 Fc Chimera (Catalog # 10720-T2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-135 kDa and 230-270 kDa, respectively.

Formulation, Preparation and Storage

10720-T2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-2

Tie-2, also known as Angiopoietin-1 receptor and Tek, along with the closely related Tie1, are vascular-specific receptor tyrosine kinases (RTKs) involved in angiogenesis, vascular development, and hematopoiesis (1, 2). The Tie molecules are characterized by three immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains, and three fibronectin type III-like repeats in the extracellular domain (ECD) and a split tyrosine kinase domain in the cytoplasmic region (3, 4). The ECD of canine Tie-2 shares 94% amino acid sequence identity with human Tie-2. The ECD of human Tie-2 is known to be cleaved from the cell surface, releasing a ~75 kDa soluble Tie-2 (5).  Tie-2 is expressed primarily on endothelial and hematopoietic progenitor cells and plays central roles in both developmental and tumor-induced angiogenesis of the adult vascular system (6). Tie receptor signaling is modulated by angiopoietins (Ang), a family secreted, multimeric growth factor ligands (2). Tie-2 signaling is activated by Ang1 and inhibited by Ang2, whereas Tie1 is considered an orphan receptor with no known ligands (7). Tie-2 and Tie1 can form heteromeric complexes in cells that express both receptors and this complex might attenuate signaling from Tie-2 (1, 8, 9).  Tie-2 overexpression has been documented in breast, ovarian and hepatocellular tumors, as well as in glioblastomas and modulation of the Tie signaling pathway has been a target for the treatment of numerous diseases and cancers (10, 11).

References

  1. Zhang, Y. et al. (2019) iScience. 20:497
  2. Eklund, L. and Saharinen, P. (2013) Exp Cell Res 319:1271. 
  3. Seegar, T.C.M. et al. (2010). Molec Cell. 37: 643.
  4. Barton, W.A. et al. (2006). Nat Struc & Molec Biol. 13:524.
  5. Findley, C.M. et al. (2007) Arterioscler Thromb. Vasc. Biol. 12:2619.
  6. Huang, H. et al. (2010) Nat. Rev. Cancer 10:575
  7. Saharinen, P. et al. (2005) J. Cell Biol. 169:239.
  8. Leppanen, V. et al. (2017) PNAS. 114:4376.
  9. Song, S. et al. (2012) Biochem and Biophy Res. Comm. 419:281.
  10. Saharinen, P. et al. (2017) Nat. Rev. Drug Discov. 16:635.
  11. Grenga, I. et al. (2015) J. Immunotherapy Cancer 3:52.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Alternate Names

CD202b, TEK, Tie2

Entrez Gene IDs

7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)

Gene Symbol

TEK

UniProt

Product Documents for Recombinant Canine Tie-2 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Canine Tie-2 Fc Chimera Protein, CF

For research use only

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