Rat CX3CL1/Fractalkine DuoSet ELISA

Name: Rat CX3CL1/Fractalkine DuoSet ELISA
Brand: R&D Systems
SKU: DY537
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY537

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Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	195 - 12,500 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Rat CX3CL1/Fractalkine DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Rat CX3CL1/Fractalkine DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant rat CX3CL1/Fractalkine. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Rat CX3CL1/Fractalkine DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Rat
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Rat CX3CL1/Fractalkine DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Rat CX3CL1/Fractalkine DuoSet ELISA

	Rat CX3CL1 / Fractalkine ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CX3CL1/Fractalkine

CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.

Item	Value
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names	ABCD-3, C3Xkine, CX3C membrane-anchored chemokine, CX3CL1, CXC3, CXC3C, FKN, Fractalkine, NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, NTTSmall-inducible cytokine D1, Neurotactin, SCYD1C-X3-C motif chemokine 1, chemokine (C-X3-C motif) ligand 1, neurotactin), small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Rat CX3CL1/Fractalkine DuoSet ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Rat CX3CL1/Fractalkine DuoSet ELISA include:

Species: Rat
Sample Types: Aqueous Humor

Yeh P et al. (2016), Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats. PLoS ONE, 11(1):e0146438.
PMID: 26765843
Species: Rat
Sample Types: Cell Culture Supernates

Cook A et al. (2010), Interactions between chemokines: regulation of fractalkine/CX3CL1 homeostasis by SDF/CXCL12 in cortical neurons. J. Biol. Chem., 285(14):10563-71.
PMID: 20124406
Species: Rat
Sample Types: Cell Culture Supernates

Husberg C et al. (2008), Cytokine expression profiling of the myocardium reveals a role for CX3CL1 (fractalkine) in heart failure. J. Mol. Cell. Cardiol., 45(2):261-9.
PMID: 18585734

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Customer Reviews for Rat CX3CL1/Fractalkine DuoSet ELISA (2)

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Rat CX3CL1/Fractalkine DuoSet ELISA

Name: Anonymous

Sample Tested: Recombinant protein

Verified Customer | Posted 11/20/2016
Rat CX3CL1/Fractalkine DuoSet ELISA

Name: Anonymous

Sample Tested: Cell Culture Media

Verified Customer | Posted 07/06/2016

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Product Documents for Rat CX3CL1/Fractalkine DuoSet ELISA

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Rat CX3CL1/Fractalkine DuoSet ELISA

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Summary for Rat CX3CL1/Fractalkine DuoSet ELISA

Product Specifications for Rat CX3CL1/Fractalkine DuoSet ELISA

Kit Contents for Rat CX3CL1/Fractalkine DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Rat CX3CL1/Fractalkine DuoSet ELISA

Preparation and Storage

Background: CX3CL1/Fractalkine

Citations for Rat CX3CL1/Fractalkine DuoSet ELISA (3)

Customer Reviews for Rat CX3CL1/Fractalkine DuoSet ELISA (2)

Customer Images

Rat CX3CL1/Fractalkine DuoSet ELISA

Rat CX3CL1/Fractalkine DuoSet ELISA

Product Documents for Rat CX3CL1/Fractalkine DuoSet ELISA

Product Datasheets

COA

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