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Human B7-2/CD86 DuoSet ELISA

Bio-Techne includes R&D Systems | Catalog # DY141-05

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DY141-05

Ancillary Products Available

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

46.9-3000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human B7-2/CD86 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Product Summary for Human B7-2/CD86 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human B7-2. The suggested diluent is suitable for the analysis of most cell culture supernate, tissue lysates, serum, and plasma samples. Diluents for complex matrices, such as serum, and plasma should be evaluated prior to use in this DuoSet.
 

Product Specifications

Assay Format

96-well strip plate (sold separately)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Label

HRP

Scientific Data Images for Human B7-2/CD86 DuoSet ELISA

Human B7-2/CD86 ELISA Standard Curve

Kit Contents for Human B7-2/CD86 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer:(Catalog #WA126), or 0.05% Tween®20 in PBS, pH 7.2-7.4

Reagent Diluent:(Catalog #DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution:1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog #DY999)

Stop Solution:2 N H2SO4(Catalog #DY994)

Microplates: R&D Systems (Catalog #DY990)

Plate Sealers:ELISA Plate Sealers (Catalog #DY992)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: B7-2/CD86

B7-2/CD86 and the related B7-1/CD80 are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. B7-2 is highly expressed on activated antigen presenting cells and vascular endothelial cells. It binds to CD28 on T cells to enhance T cell activation. It additionally binds CTLA-4 which inhibits the T cell response and supports regulatory T cell function. The relative participation of B7-1 and B7-2 in T cell co-stimulation can alter the Th1/Th2 bias of the immune response. Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses.

Alternate Names

B72, CD86

Entrez Gene IDs

942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)

Gene Symbol

CD86

Additional B7-2/CD86 Products

Product Documents for Human B7-2/CD86 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human B7-2/CD86 DuoSet ELISA

For research use only

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