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ExCellerate Human T Cell Expansion Media, Xeno-Free
R&D Systems, Inc. a Bio-Techne Brand
Catalog # CCM030
Key Product Details
| Features: | Media for the ex vivo expansion and culture of human T cell lymphocytes under xeno-free conditions. |
| Key Benefits: |
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Summary for ExCellerate Human T Cell Expansion Media, Xeno-Free
Why Use ExCellerate Human T Cell Expansion Media?
T Lymphocytes (T Cells) are a critical component of the adaptive immune response. While dysregulation of T cell function and proliferation contributes to the etiology of many diseases, the ability of T cells to activate an immune response toward specific antigens is being harnessed as a powerful immunotherapy tool to combat cancer and other diseases. To facilitate discovery and preclinical research for T cell immunoregulation and therapy, robust platforms for ex vivo expansion and maintenance of T cells, including specialized serum-free or xeno-free media, are required.
ExCellerate Human T Cell Expansion Media is formulated and optimized for the ex vivo culture of human T lymphocytes in research applications. Unlike traditional serum-containing culture media, xeno-free media provides a stable culture and optimized environment, void of non-human animal-derived products, that facilitates the generation and expansion of T cells. The medium supports expansion of routine culture of human T cell lines or T cell clones and the stimulation of human peripheral blood lymphocytes.
Versatility of ExCellerate Human T Cell Expansion Media.
ExCellerate Human T Cell Expansion Media is optimized for T cell expansion when used in combination with Recombinant Human Cytokines (IL-2 or IL-7 and IL-15) and cell activation methods, including particle, bead-based, or plate-bound anti-CD3/anti-CD28 antibodies. The activation and cytokine/growth factor combinations used with this media should be optimized by application or experimental protocol.
Product Details
- 500 mL of ExCellerate Human T Cell Expansion Media
Data Examples
Fold Expansion of Human T Cells in ExCellerate Human T Cell Expansion Media. Primary human peripheral blood mononuclear cells (PBMCs) were cultured for 9 days in ExCellerate Human T Cell Expansion Media using activating beads and Recombinant Human IL-2 (R&D Systems, Catalog # 202-IL). Cell counts were performed to determine fold expansion compared to the Day 0 seeding density (0.25 x 106 cells/mL).
Phenotypic Analysis of Human T cells Expanded in Xeno-Free Human T Cell Expansion Media. Human PBMCs were cultured for 9 days in ExCellerate Human T Cell Expansion Media using activating beads and Recombinant Human IL-2 (R&D Systems, Catalog # 202-IL). Human T Cells were enriched during the culture as indicated via the expression of positive T cell markers, including Mouse anti-Human CD3 epsilon APC-conjugated Antibody (Catalog # FAB100A) and with a Mouse Anti-Human CD8 alpha PE-conjugated Antibody (Catalog # FAB1509).
ExCellerate Human T Cell Expansion Media Enhances T Cell Expansion Compared to Other Commercial Media. 2.5 x 106 primary human peripheral blood mononuclear cells (PBMCs) were cultured for 9 days using ExCellerate Human T Cell Expansion Media or other commercially available media (Supplier #1 or #2), in combination with Recombinant Human IL-2 GMP (Catalog # 202-GMP), Recombinant Human IL-15 (Catalog # 247-GMP), Recombinant Human IL-7 GMP (Catalog # 207-GMP). The total number of T cells (A) and CD8+ T cells (B) were determined at days 5 and 9. ExCellerate Human T Cell Expansion Media shows superior expansion rates compared to other commercial media.
Performance of GMP Cytokines in ExCellerate Human T Cell Expansion Media. Primary human peripheral blood mononuclear cells (PBMCs) were cultured for 12 days in ExCellerate Human T Cell Expansion Media or another commercially available T cell media (Supplier #1) containing combinations of Recombinant Human IL-2 GMP (Catalog # 202-GMP), Recombinant Human IL-15 (Catalog # 247-GMP), Recombinant Human IL-7 GMP (Catalog # 207-GMP). The total number of T cells were quantified at days 0, 5, 8, and 12. Under all GMP cytokine conditions, T cells cultured using ExCellerate Human T Cell Expansion Media showed superior expansion compared to Supplier #1 media.
Superior Human T Cell Expansion Using ExCellerate Human T Cell Expansion Media and Plate-bound Anti-CD3 and Anti-CD28 Antibody Stimulation. Primary human peripheral blood mononuclear cells (PBMCs) were cultured for 15 days in ExCellerate Human T Cell Expansion Media or other commercially available media (Supplier #1 or #2), in combination with Recombinant Human IL-2 (R&D Systems, Catalog # 202-IL) and plate-bound Mouse Anti-Human CD3 epsilon Antibody (Catalog # MAB100) and Human CD28 Antibody (Catalog # AF-342-PB). Fold expansion was determined and cell counts were performed at days 5, 11, and 15 to determine fold expansion compared to the Day 0 seeding density. ExCellerate Human T Cell Expansion Media shows superior expansion rates compared to other commercial media.
Lower Exhaustion Phenotype of T Cells Cultured Using ExCellerate Human T Cell Expansion Media Compared to Other T Cell Media. Primary human peripheral blood mononuclear cells (PBMCs) were cultured (0.4 x 106 cells/mL) for 9 days using either ExCellerate Human T Cell Expansion Media or another commercially available T cell expansion media (Supplier 1). Both media were supplemented with Recombinant Human IL-2 (R&D Systems, Catalog # 202-IL) and activating microbeads. (A) At days 5, 7, and 9, T cells fold expansion was determined determined. (B) T cells were resuspended in their respective media (0.4 x 106 cells/mL), restimulated in culture with activating beads for an additional 6 days. At days 12 and 15 in culture, cells restimulated in ExCellerate Human T Cell Expansion Media showed continued expansion of T cells compared to the lower expansion rates of cell cultured in Supplier 1 media. (C) T cells were analyzed by flow cytometry for expression of PD-1, TIM3, LAG-1, and TIGIT, common surface markers of cell exhaustion. CD4+ T cells restimulated and cultured using ExCellerate Human T Cell Expansion Media showed low expression of cell exhaustion markers. Exhaustion markers on cells grown in Supplier 1 media show elevated expression levels.
HSC Differentiation & |
The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long maintenance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continuously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage depletion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.
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