Recombinant Human IL-15 GMP Protein, CFGMP

Name: Recombinant Human IL-15 GMP Protein, CF
Brand: rnd
SKU: 247-GMP

Catalog # 247-GMP

Citations (1)

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Key Product Details

Source:	E. coli
Accession #:	P40933
Applications:	Bioactivity

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Product Specifications

Source	E. coli-derived human IL-15 protein Asn49-Ser162 Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.
Purity	>97%, by SDS-PAGE with silver staining, under reducing conditions.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asn-Trp-Val-Asn-Val-Ile-Ser-Asp-Leu-Lys
Predicted Molecular Mass	12.5 kDa
Activity	Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is 0.3-2.6 ng/mL. The specific activity of recombinant human IL-15 is >2.0 x 10⁸ units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554).

Recombinant Human IL-15 GMP Protein, CF Scientific Data Examples

	Recombinant Human IL-15 GMP Protein Bioactivity GMP-grade Recombinant Human IL-15 (Catalog # 247-GMP) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED₅₀ for this effect is 0.3-2.6 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP IL-15.
	Recombinant Human IL-15 GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human IL-15 (Catalog # 247-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.
	Recombinant Human IL-15 GMP Protein Mass Spectrometry MALDI-TOF analysis of GMP-grade Recombinant Human IL-15 (Catalog # 247-GMP). The major peak corresponds to the calculated molecular mass, 12774 Da. The minor peak at 13006 Da is a matrix-associated artifact of the MALDI-TOF.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

247-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). IL-15 binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (7). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8⁺ memory cells (12 - 16). An important component of these functions is the ability of
IL-15 to induce dendritic cell differentiation and inflammatory activation (11, 14). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8⁺ T cells (17). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (18).

Item	Value
References	De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19. Grabstein, K. et al. (1994) Science 264:965. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444. Giri, J.G. et al. (1995) EMBO J. 14:3654. Giri, J. et al. (1994) EMBO J. 13:2822. Dubois, S. et al. (2002) Immunity 17:537. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368. Mortier, E. et al. (2004) J. Immunol. 173:1681. Bergamaschi, C. et al. (2012) Blood 120:e1. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192. Mortier, E. et al. (2003) J. Exp. Med. 205:1213. Gordy, L.E. et al. (2011) J. Immunol. 187:6335. Harris, K.M. (2011) J. Leukoc. Biol. 90:727. Xia, J. et al. (2010) Clin. Immunol. 134:130. Schluns, K.S. et al. (2002) J. Immunol. 168:4827. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529. Barra, N.G. et al. (2010) Obesity 18:1601.
Long Name	Interleukin 15
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names	IL-15, IL-15MGC9721, IL15, interleukin 15, interleukin-15

Citations (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-15 GMP Protein, CF include:

Species: Human
Sample Types: Whole Cell
Applications: Cell Culture

A Pfefferle et al. (2019), Intra-lineage Plasticity and Functional Reprogramming Maintain Natural Killer Cell Repertoire Diversity Cell Rep, 29(8):2284-2294.e4.
PMID: 31747601

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Recombinant Human IL-15 GMP Protein, CFGMP

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Product Specifications

Recombinant Human IL-15 GMP Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IL-15

Citations (1)

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