Endothelial Cell Growth Media

Endothelial Cell Growth Media Supports Cell Proliferation of Human Umbilical Cord Endothelial Cells (HUVECs). One T25 flask of HUVECs (0.17 x 10⁶ cells) were expanded over 10 passages using Complete Endothelial Cell Growth Media. Cells were passaged every 4-6 days. The histogram shows the cumulative number of HUVECs expanded over 10 passages if all cells were replated at each passage.

Human Umbilical Cord Endothelial Cells (HUVECs) Express Endothelial Cell Markers CD31, KDR, and vWF. HUVECs were expanded in Complete Endothelial Cell Growth Media for 5 passages prior to staining for marker expression. (A,B) Flow cytometry analysis of endothelial cell marker expression. Filled histograms indicate cells stained with PE-conjugated Mouse Anti-CD31 (A; R&D Systems^®, Catalog # FAB3567P) or PE-conjugated Mouse Anti-KDR (B; R&D Systems^®, Catalog # FAB357P). Open histograms in A and B indicate staining with PE-conjugated Mouse IgG1 isotype control (R&D Systems^®, Catalog # IC002P). (C) Immunocytochemistry analysis of endothelial cell marker expression. Cells were double stained with Mouse Anti-CD31 (red; R&D Systems^®, Catalog # BBA7) and Rabbit Anti-Von Willebrand Factor (vWF) (green; Novus Biologicals^®, Catalog # NB600-586). The nuclei were counterstained with DAPI (blue).

Increased HUVEC Survival and CD31 Expression Compared to Competitor Endothelial Cell Media. HUVECs were expanded for 14 passages in either R&D Systems Endothelial Cell Growth Media (Catalog # CCM027) or competitor endothelial cell media. Cells were stained for CD31 expression using the Mouse Anti-Human CD31 Antibody (yellow; R&D Systems; Catalog # BBA7) and counterstained with DAPI (Catalog # 5748). A) Representative images of CD31 expression in p14 HUVECs grown in R&D Systems Media or competitor media. There is a greater density of cells and a higher expression of CD31 in HUVECs grown in R&D Systems Media. B) Histogram showing that at p14, there is a higher percentage of surviving cells that express CD31 in HUVECs grown in R&D Systems Media compared to competitor.

Improved Yield of Murine CD31⁺PDGFα^- Lung Cells using Endothelial Cell Growth Media. A single cell suspension of mouse lung cells was prepared and seeded onto gelatin coated plates in 3 different media, R&D Systems^® Endothelial Cell Growth Media (Catalog # CCM027), competitor media, or 20% FBS/DMEM. After one week in culture, cells were analyzed for expression of CD31 (APC) and PDGFRα (PE). The percentage of CD31+PDGFRα- cells obtained from R&D Systems Endothelial Cell Growth Media was superior (13.2%) compared to competitor (3.5%) and 20% FBS/DMEM (4.5%) media. Data courtesy of the laboratory of Dr. Michael Kyba at the University of Minnesota.

Improved Proliferation of Primary Human Dermal Lymphatic Endothelial Cells. Human dermal lymphatic endothelial cells (HDLECs) were cultured in either Endothelial Cell Growth Media (R&D Systems, Catalog # CCM027) or a competitor endothelial cell media. After 48 hours, HDLEC proliferation was assessed using a MTT proliferation assay. HDLECs cultured in R&D Systems Endothelial Cell Growth Medium showed a 24% increase in proliferation compared to competitor media. The P-value was calculated using an unpaired t-test. Data provided courtesy of the laboratory of Dr. Constantinos Mikelis at Texas Tech University Health Sciences Center.