Endothelial Cell Growth Media
Order Information
Shipping Information
$35 to United States
Products in stock will be shipped in 1-3 business days unless specified.
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, Complete Endothelial Cell Growth Media is prepared using the following procedure:
- Add Endothelial Growth Media Supplement to Endothelial Cell Growth Base Media
- Store completed media at 2 - 8 °C
Reagents provided with the Endothelial Cell Growth Media (Catalog # CCM027):
- Endothelial Cell Growth Base Media Human
- Endothelial Cell Growth Supplement (50X)
Note: The components of this list require different storage/shipping temperatures and will arrive in separate packaging.
Reagents
- HUVECs
- Penicillin-Streptomycin (100X), optional
- TrypLE™ Express
- Phosphate-Buffered Saline (PBS) (Tocris; Catalog # 3156)
Materials
- 75 cm2 tissue culture (T75) flasks
- 15 mL centrifuge tubes
- Serological pipettes
- Pipette and pipette tips
Equipment
- 37°C and 5% CO2 humidified incubator
- Centrifuge (low speed clinical or equivalent)
- Hemocytometer
- Inverted microscope
- Water bath
Reagent Preparation
Endothelial Cell Growth Supplement - Thaw the Endothelial Cell Growth Supplement (50X) at 2-8 °C or room temperature.
Complete Endothelial Cell Growth Media - Add 5 mL of Endothelial Cell Growth Supplement to 250 mL of Endothelial Cell Growth Base Media. Add Penicillin-Streptomycin at a 1:100 dilution. Store under sterile conditions at 2-8 °C for up to 2 weeks.
Note: If Penicillin-Streptomycin is not needed for the experiment, it can be omitted.
Culturing of HUVECs
Warm Complete Endothelial Cell Growth Media to 37 °C.
Determine the size and number of flasks needed for plating.

Warm the frozen vial of HUVECs until just thawed.

Transfer cells immediately and gently into a 15 mL centrifuge tube containing > 5 mL of prewarmed Complete Endothelial Cell Growth Media.
Centrifuge at 200 x g for 5 minutes.

Resuspend the pellet in prewarmed Complete Endothelial Cell Growth Media
Transfer HUVEC suspension into a T75 flask(s).

Change media the day following thaw.
Monitor cells daily and change media every other day.
Passage the cells at 80-90% confluency.

Subculturing of HUVECs
Warm Complete Endothelial Cell Growth Media to 37 °C.

Remove and discard the media from the flasks.
Rinse cells once with sterile 1X PBS.
Digest cells with TrypLE Express for 1-2 minutes at 37 °C.

Tap the side of the flask to aid the detachment of the cells.
Transfer the cell suspension to a 15 mL centrifuge tube containing 8 mL of prewarmed Complete Endothelial Cell Growth
Centrifuge at 200 x g for 5 minutes.

Plate cells (approximately 6.7 x 103 cells/cm2).
Change media the day following thaw.
Monitor cells daily and change media every other day.
Passage the cells at 80-90% confluency.

Citations (1)
Citations are publications that use Bio-techne products. Selected citations for Endothelial Cell Growth Media include:
-
Species: Human
Sample Types: Whole Cells
Wu Z et al. (2017), Imaging specific cellular glycan structures using glycosyltransferases via click chemistry. Glycobiology, 0(0):.
PMID: 29186441
There are no citations that match your criteria.
Customer Reviews (6)
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Endothelial Cell Growth Media
Verified Customer | Posted 11/05/2018 -
Endothelial Cell Growth Media
Verified Customer | Posted 07/11/2018 -
Endothelial Cell Growth Media
Verified Customer | Posted 04/03/2018 -
Endothelial Cell Growth Media
Verified Customer | Posted 02/03/2018Im working with HUVEC cells for the first time. I was very confused about the medium, so I ordered this medium. It was delivered on time and had needed growth factor supplements for HUVEC. I will highly recommend this medium. -
Endothelial Cell Growth Media
Verified Customer | Posted 01/11/2018Our primary retina endothelial cells grow fast and nice with this media. -
Endothelial Cell Growth Media
Verified Customer | Posted 12/27/2017We culture primary Primary Human Retinal Microvascular Endothelial Cells. The cell grow very well by meida and 50X supplement.
There are no reviews that match your criteria.