Human CD31/PECAM-1 Antibody

Detection of CD31/PECAM-1 in Artery in Human Liver.

CD31/PECAM-1 was detected in immersion fixed paraffin-embedded sections of Artery in Human Liver using Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human CD31/PECAM-1 by Immunocytochemistry/ Immunofluorescence

Characterization&functional analysis of expanded AEPCs compared with HUVECs. All the experiments performed using AEPCs&HUVECs at passage number 5. (A) Fluorescent immunocytochemistry of the expression profiles of endothelial cell-specific markers, CD31, vWF,&the binding capacity of isolectin-B4, in AEPCs&HUVECs. Nuclear staining was assessed by 4′,6-diamidino-2-phenylindole (DAPI). Photographs taken using a confocal microscope (OLYMPUS, FV1000 with a CCD camera DP71&an objective lens UPlanFL N 40×/1.30 oil; 400× magnification) as a plane image. Bars represent 100 µm. The graphs show the %age of endothelial marker-positive cells among DAPI-positive cells. The data represent two independent experiments (2 donors),&%ages determined from cultivations performed in triplicate. The bars represent means ± SD (n = 6, **P < 0.01, two-tailed unpaired t-test). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35110646), licensed under a CC-BY license. Not internally tested by R&D Systems.