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Human CD31/PECAM‑1 Antibody
Catalog # BBA7 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 9G11
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Product Summary for Human CD31/PECAM‑1 Antibody
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Specificity
Detects human CD31/PEACAM-1. In Western blots, no cross-reactivity with recombinant human (rh) E-Selectin, rhICAM-1, -2, -3, rhVCAM‑1, or recombinant mouse VCAM‑1 was observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD31/PECAM‑1 Antibody
Detection of Human CD31/PECAM‑1 by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD31/PECAM‑1 in HUVEC Human Cells.
CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
CD31/PECAM‑1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.
CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human CD31/PECAM‑1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Artery in Human Liver
Sample: Immersion fixed paraffin-embedded sections of Artery in Human Liver
Immunoprecipitation
Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.
Knockout Validated
CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line.
Western Blot
1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells
Sample: HUVEC human umbilical vein endothelial cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 7 reviews rated 4.1 using BBA7 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD31/PECAM-1
Product Documents for Human CD31/PECAM‑1 Antibody
Product Specific Notices for Human CD31/PECAM‑1 Antibody
For research use only
Citations for Human CD31/PECAM‑1 Antibody (36)
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Citations are publications that use Bio-Techne products. Selected citations for Human CD31/PECAM‑1 Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: ICCM Thakar et al. (2023-04-07), Microvascular Environment Influences Brain Microvascular Heterogeneity: Relative Roles of Astrocytes and Oligodendrocytes for the EPCR Expression in the Brain Endothelium International Journal of Molecular Sciences, 2023-024(8).
PMID: 37108071 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCS Johnson et al. (2022-05-18), PARG suppresses tumorigenesis and downregulates genes controlling angiogenesis, inflammatory response, and immune cell recruitment BMC Cancer, 2022-022(1):557.
PMID: 35585513 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCDA Nawrot et al. (2022-04-28), A Novel High Content Angiogenesis Assay Reveals That Lacidipine, L-Type Calcium Channel Blocker, Induces In Vitro Vascular Lumen Expansion International Journal of Molecular Sciences, 2022-023(9).
PMID: 35563280 -
Species: Human
Sample Types: Whole Cells
Applications: ICCN Saito et al. (2022-02-02), Purification and characterization of human adipose-resident microvascular endothelial progenitor cells Scientific Reports, 2022-012(1):1775.
PMID: 35110646 -
Species: Human
Sample Types: Whole Tissue
Applications: Functional Assay,IFZ Zhang et al. (2021-10-23), Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease? Aging Cell, 2021-10(0):e13501.
PMID: 34687487 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCRM Urbantat et al. (2021-10-16), Tumor-Associated Microglia/Macrophages as a Predictor for Survival in Glioblastoma and Temozolomide-Induced Changes in CXCR2 Signaling with New Resistance Overcoming Strategy by Combination Therapy International Journal of Molecular Sciences, 2021-122(20).
PMID: 34681839 -
Species: Human,Rhesus Macaque
Sample Types: Whole Tissue
Applications: IHCJR Dayton et al. (2021-09-07), Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation Frontiers in Cellular Neuroscience, 2021-015(0):683687.
PMID: 34557075 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCK Goel et al. (2021-05-14), Sphingosine 1 Phosphate (S1P) Receptor 1 Is Decreased in Human Lung Microvascular Endothelial Cells of Smokers and Mediates S1P Effect on Autophagy Cells, 2021-010(5).
PMID: 34068927 -
Species: Human
Sample Types: Whole Cells
Applications: ICCU Roy Chowdh et al. (2021-02-17), Isolation and characterization of novel primary cells from the human distal outflow pathway Scientific Reports, 2021-011(1):4034.
PMID: 33597641 -
Species: Human
Sample Types: Organoid
Applications: IHCK Uchimura et al. (2020-12-15), Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling Cell Reports, 2020-133(11):108514.
PMID: 33326782 -
Species: Human
Sample Types: Whole Cells
Applications: ICCRA Santos et al. (2020-12-15), Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow International Journal of Molecular Sciences, 2020-121(24).
PMID: 33334068 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryC Manferdini et al. (2020-12-10), Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis Biology, 2020-19(12).
PMID: 33321965 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCN Mori et al. (2020-04-14), Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue Sci Rep, 2020-010(1):5646.
PMID: 32286353 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-FrR Assaly et al. (2019-09-21), Extracorporeal Shock Waves Therapy Delivered by Aries Improves Erectile Dysfunction in Spontaneously Hypertensive Rats Through Penile Tissue Remodeling and Neovascularization Sex Med, 2019-00(0).
PMID: 31551177 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotT Tsutsumi et al. (2019-07-24), Nintedanib ameliorates experimental pulmonary arterial hypertension via inhibition of endothelial mesenchymal transition and smooth muscle cell proliferation PLoS ONE, 2019-014(7):e0214697.
PMID: 31339889 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrJ Luzuriaga et al. (2019-03-28), Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate Into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain Front Physiol, 2019-010(0):347.
PMID: 30984027 -
Species: Mouse,Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHCJ Rose Dayto et al. (2019-02-21), Straightforward method for singularized and region-specific CNS microvessels isolation J. Neurosci. Methods, 2019-00(0).
PMID: 30797797 -
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICCG Ecklu-Mens et al. (2018-10-09), Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes PLoS ONE, 2018-113(10):e0204177.
PMID: 30300360 -
Species: Human
Sample Types: Whole Cells
Applications: ICCS Li et al. (2017-08-07), Hydrogels with precisely controlled integrin activation dictate vascular patterning and�permeability Nat Mater, 2017-016(9):953-961.
PMID: 28783156 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryK Yamamizu et al. (2017-02-23), In�Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling Stem Cell Reports, 2017-00(0).
PMID: 28238797 -
Species: Human
Sample Types: Whole Cells
Applications: ICCLopa Leach (2016-10-21), Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms Placenta, 2016-148(0):87-98.
PMID: 27871478 -
Species: Human
Sample Types: Whole Cells
Applications: IHC-P(2016-08-17), The ACVR1 R206H mutation found in fibrodysplasia ossificans progressiva increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling Stem Cell Res Ther, 2016-07(1):115.
PMID: 27530160 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC(2016-07-18), Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis Sci Rep, 2016-06(0):29884.
PMID: 27425816 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayY Chen et al. (2016-03-08), Endothelial Surface Protrusion by a Point Force Biophys. J, 2016-0110(5):1150-7.
PMID: 26958891 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCWeston et al. (2014-12-22), Vascular adhesion protein-1 promotes liver inflammation and drives hepatic fibrosis. J Clin Invest, 2014-1125(2):501-20.
PMID: 25562318 -
Species: Human,Rat
Sample Types: Whole Cells
Applications: IHC,Flow CytometryMartinelli R et al. (2014-07-07), Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance. J Cell Sci, 2014-0127(0):3720-34.
PMID: 25002404 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PLiu T et al. (2014-06-11), Association between PON1 rs662 polymorphism and coronary artery disease. Eur J Clin Nutr, 2014-068(9):1029-35.
PMID: 24918121 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCKanasaki K et al. (2014-02-26), Linagliptin-mediated DPP-4 inhibition ameliorates kidney fibrosis in streptozotocin-induced diabetic mice by inhibiting endothelial-to-mesenchymal transition in a therapeutic regimen. Diabetes, 2014-063(6):2120-31.
PMID: 24574044 -
Species: Human
Sample Types: Whole Cells
Applications: ICCWilliamson MR et al. (2007-09-11), The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment. Biomaterials, 2007-028(35):5307-18.
PMID: 17850863 -
Species: Human
Sample Types: Whole Cells
Applications: ICCDentelli P et al. (2007-02-08), C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium. Blood, 2007-0109(10):4264-71.
PMID: 17289809 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: ICC,Western BlotNeria F et al. (2006-10-11), Mechanisms of endothelial cell protection by blockade of the JAK2 pathway. Am. J. Physiol., Cell Physiol., 2006-1292(3):C1123-31.
PMID: 17035297 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCManchio JV et al. (2005-06-01), Disruption of graft endothelium correlates with early failure after off-pump coronary artery bypass surgery. Ann. Thorac. Surg., 2005-079(6):1991-8.
PMID: 15919298 -
Species: Human
Sample Types: Whole Cells
Applications: ICCCarman CV et al. (2004-10-25), A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them. J. Cell Biol., 2004-1167(2):377-88.
PMID: 15504916 -
Species: Human
Sample Types: Whole Cells
Applications: Western Blot,ICCWei H et al. (2004-08-06), Identification of a novel transcript of human PECAM-1 and its role in the transendothelial migration of monocytes and Ca2+ mobilization. Biochem. Biophys. Res. Commun., 2004-0320(4):1228-35.
PMID: 15249221 -
Species: Human
Sample Types: Whole Cells
Applications: ICCSatchell SC et al. (2004-03-01), Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties. J. Am. Soc. Nephrol., 2004-015(3):566-74.
PMID: 14978158 -
Species: Human
Sample Types: Whole Cells
Applications: ICCGomez D et al. (2003-06-01), Stimulation of primary human endothelial cell proliferation by IFN. J. Immunol., 2003-0170(11):5373-81.
PMID: 12759411
There are no citations that match your criteria.
Customer Reviews for Human CD31/PECAM‑1 Antibody (7)
4.1 out of 5
7 customer
ratings
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Application: Western BlotSample Tested: HUVEC human umbilical vein endothelial cellsSpecies: HumanVerified Customer | Posted 03/09/2022
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/14/2021
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/14/2018
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/02/2018
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: HUVEC human umbilical vein endothelial cellsSpecies: HumanVerified Customer | Posted 02/28/2017Antibody was reconstituted at 0.5mg/ml and used at 1:50 dilution overnight in 4ºC.
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Application: ImmunohistochemistrySample Tested: arterySpecies: HumanVerified Customer | Posted 08/29/2016Frozen human artery. Permeabilized with 80% acetone. Primary antibody at 1:100 incubate at 4oC overnight Secondary antibody: CY3 donkey anti-mouse secondary antibody. 1 hour at room temperature.
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Application: ImmunohistochemistrySample Tested: Muscle tissueSpecies: HumanVerified Customer | Posted 08/29/2016Antigen retrieval: 10mM Citrate pH6. Primary antibody at 1:100 incubate at 4oC overnight Secondary antibody: Alexa 647 donkey anti-mouse secondary antibody. 1 hour at room temperature.
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