Human CD31/PECAM‑1 PE‑conjugated Antibody

Name: Human CD31/PECAM‑1 PE‑conjugated Antibody
Brand: R&D Systems
SKU: FAB3567P
Rating: 4 (2 reviews)

Catalog # FAB3567P | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (10)

Product Datasheet / COA / SDS

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Technical Data
Citations & Reviews

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Knockout Validated

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # 9G11

Product Summary for Human CD31/PECAM‑1 PE‑conjugated Antibody

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

Detects human CD31/PECAM‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) E‑Selectin, rhICAM-1, -2, -3, rhVCAM-1, or recombinant mouse VCAM-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human CD31/PECAM‑1 PE‑conjugated Antibody

Detection of CD31/PECAM‑1 in Human Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CD31/PECAM-1 in THP-1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (IC002P, open histogram). Staining was performed using our Membrane-Associated Staining protocol.

CD31/PECAM-1 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

CD31/PECAM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (IC002P, open histogram). No staining in the CD31/PECAM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CD31/PECAM‑1 PE‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood granulocytes and THP-1 human acute monocytic leukemia cell line

Knockout Validated

CD31/PECAM-1 is specifically detected in THP-1 myelogenous leukemia parental cell line but is not detectable in CD31/PECAM-1 knockout THP-1 cell line.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using FAB3567P in the following applications:

Flow Cytometry (2 Reviews)

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alphav beta3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Gene Symbol

PECAM1

Product Documents for Human CD31/PECAM‑1 PE‑conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD31/PECAM‑1 PE‑conjugated Antibody

For research use only

Citations
Reviews

Citations for Human CD31/PECAM‑1 PE‑conjugated Antibody (10)

Showing 1 - 10 of 10 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human CD31/PECAM‑1 PE‑conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Masuda et al. (2023-06-01), GATA6 regulates anti-angiogenic properties in human cardiac fibroblasts via modulating LYPD1 expression Regenerative therapy
PMID: 37251737
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Y Basmaeil et al. (2021-12-10), Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro Cells, 2021-110(12).
PMID: 34944000
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

A Inui et al. (2019-10-22), Generation of a large-scale vascular bed for the inï¿½vitro creation of three-dimensional cardiac tissue Regen Ther, 2019-111(0):316-323.
PMID: 31687425
Species: Human
Sample Types: Whole Cells
Applications: ICC,Cell Selection

S Masuda et al. (2018-07-07), Preparation of iPS cell-derived CD31+ endothelial cells using three-dimensional suspension culture Regen Ther, 2018-09(0):1-9.
PMID: 30525069
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Kryza et al. (2018-04-20), Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix Sci Rep, 2018-08(1):6331.
PMID: 29679011
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Norton et al. (2014-09-09), Antiangiogenic cancer drug sunitinib exhibits unexpected proangiogenic effects on endothelial cells. Onco Targets Ther, 2014-07(0):1571-82.
PMID: 25228815
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Kryza T et al. (2013-11-13), Angiogenesis stimulated by human kallikrein-related peptidase 12 acting via a platelet-derived growth factor B-dependent paracrine pathway. FASEB J, 2013-128(2):740-51.
PMID: 24225148
Species: Human
Sample Types: Cell Lysates
Applications: Flow Cytometry

Frassanito M et al. (2013-09-02), Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies. 2013-00(0).
PMID: 23995611
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Albert R et al. (2012-10-09), Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium. PLoS ONE, 2012-17(10):e47187.
PMID: 23056608
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kelly MA et al. (2009-02-12), Signaling hierarchy regulating human endothelial cell development. Arterioscler. Thromb. Vasc. Biol., 2009-029(5):718-24.
PMID: 19213939

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