TGF-beta 3 Antibody

Name: TGF-beta 3 Antibody
Brand: R&D Systems
SKU: AF-243-NA

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF-243-NA

Citations (5)

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Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant chicken TGF‑ beta 3 Ala301-Ser412 (Tyr340Phe) Accession # P10600
Applications:	Block/Neutralize, Immunohistochemistry
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects TGF-beta 3 in direct ELISAs.

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Applications for TGF-beta 3 Antibody

	Recommended Concentration	Sample
Immunohistochemistry	5-15 µg/mL	Immersion fixed paraffin-embedded sections of human brain

Item	Value
Neutralization	Measured by its ability to neutralize TGF‑ beta 3 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 0.01‑0.05 µg/mL in the presence of 0.1 ng/mL Recombinant Human TGF‑ beta 3 and 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for TGF-beta 3 Antibody

	Neutralization TGF‑ beta 3 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 3 Antibody. Recombinant Human TGF-beta 3 (243-B3) inhibits Recombinant Mouse IL-4 (404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of TGF-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA). The ND₅₀ is typically 0.01-0.05 µg/mL.
	Immunohistochemistry TGF‑ beta 3 in Human Brain. TGF‑ beta 3 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-TGF‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Western Blot Detection of TGF‑ beta 3 by Western Blot. Western blot shows lysates of Saos-2 human osteosarcoma cell line, L-929 mouse fibroblast cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-TGF-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF-beta 3 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human TGF‑ beta 3 by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line and Saos-2 human osteosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for TGF-beta 3 at approximately 67 kDa (as indicated) using 10 µg/mL of Goat Anti-TGF-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: TGF-beta 3

TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8‑10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity (1). TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

Item	Value
References	Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982. Wahl, S.M. (2006) Immunol. Rev. 213:213. Chang, H. et al. (2002) Endocr. Rev. 23:787. Lin, J.S. et al. (2006) Reproduction 132:179. Hinck, A.P. et al. (1996) Biochemistry 35:8517. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261. Derynck, R. et al. (1988) EMBO J. 7:3737. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name	Transforming Growth Factor beta 3
Entrez Gene IDs	7043 (Human); 21809 (Mouse); 25717 (Rat)
Alternate Names	ARVD, ARVD1, FLJ16571, LDS5, RNHF, TGF-beta 3, TGF-beta-3, TGF-beta3, TGFB3, TGFbeta 3, transforming growth factor beta-3, transforming growth factor, beta 3

Citations for TGF-beta 3 Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for TGF-beta 3 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

I Hanson et al. (2022), Low-Dose-Rate Radiation-Induced Secretion of TGF-beta3 Together with an Activator in Small Extracellular Vesicles Modifies Low-Dose Hyper-Radiosensitivity through ALK1 Binding International Journal of Molecular Sciences, 23(15).
PMID: 35897723
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

Kang I et al. (2014), Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion. J Biol Chem, 289(22):15393-404.
PMID: 24719328
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Le M et al. (2012), Transforming growth factor Beta 3 is required for excisional wound repair in vivo. PLoS ONE, 7(10):e48040.
PMID: 23110169
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Neurohr C et al. (2006), Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure. Am. J. Respir. Cell Mol. Biol., 35(2):252-9.
PMID: 16574941
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Murphy MO et al. (2006), Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration. Eur J Vasc Endovasc Surg, 31(5):516-22.
PMID: 16427789

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TGF-beta 3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for TGF-beta 3 Antibody

Scientific Data Examples for TGF-beta 3 Antibody

Neutralization

Immunohistochemistry

Western Blot

Simple Western

Formulation, Preparation and Storage

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Background: TGF-beta 3

Citations for TGF-beta 3 Antibody (5)

Product Documents for TGF-beta 3 Antibody

Product Datasheets

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