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Recombinant Human TGF-beta 3 Protein

Catalog # 243-B3 | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
243-B3-002/CF
243-B3-010/CF

Catalog #
Availability
Size / Price
Qty
With Carrier
243-B3-002
243-B3-010

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human TGF-beta 3 protein
Ala301-Ser412 (Tyr340Phe)

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala301

Predicted Molecular Mass

12.7 kDa (monomer)

SDS-PAGE

12 kDa, reducing conditions
24 kDa, non-reducing conditions

Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is 0.0100-0.0400 ng/mL.

Scientific Data Images for Recombinant Human TGF-beta 3 Protein

Equivalent Bioactivity of Sf 21 (baculovirus)-derived and CHO-derived Recombinant Human TGF‑ beta3.

As an alternative, please consider our next generation CHO-drived Recombinant Human TGF‑ beta3 (8420-B3). It has equivalent bioactivity to Sf 21 (baculovirus)-derived Recombinant Human TGF‑ beta3 (Catalog # 243-B3). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human TGF‑ beta3 proteins are measured by their ability to inhibit the IL-4-dependent proliferation of Ht-2 mouse T cell. (orange, green, respectively).

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 243-B3
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 243-B3/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute 2 µg vials at 20 µg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. Reconstitute 10 µg or larger vials at 50 µg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 3

TGF­ beta3 (transforming growth factor-beta 3) is a member of a TGF-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and ­-beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial­-mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF-beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF-­ beta3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin­like convertase to generate a 220 aa latency­associated peptide (LAP) and a 112 aa mature TGF­-beta 3 (10, 11). Mature human TGF­-beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF-­ beta3, respectively. TGF-beta 3 is secreted as a complex with LAP. This latent form of TGF-beta 3 becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin-1, and a subset of integrins (12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-­ beta RI or ALK-­1, to modulate transcription through Smad phosphorylation (13-15). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF-­ beta RIII and endoglin) and the subsequent initiation of Smad-­dependent or -independent signaling pathways (14, 16, 17).

References

  1. Barrio, M.C. et al. (2014) Cells Tissues Organs. [Epub ahead of print; PMID 24861080].
  2. Doetschman, T. et al. (2012) Genesis 50:59.
  3. Mittl, P.R. et al. (1996) Protein Sci. 5:1261.
  4. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  5. Wahl, S.M. et al. (2006) Immunol. Rev. 213:213.
  6. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  7. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  8. Jin, J.Z. et al. (2014) Dev. Dyn. [Epub ahead of print; PMID 25104574].
  9. Tang, Q.O. et al. (2009) Expert Opin. Biol Ther. 9:689.
  10. Derynck, R. et al. (1988) EMBO J. 7:3737.
  11. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  12. Oklu, R. and R. Hesketh (2000) Biochem. J. 352 Pt 3:601.
  13. Cui, X.M. and C.F. Shuler (2000) Int. J. Dev. Biol. 44:397.
  14. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  15. Nakajima, A. et al. (2007) Dev. Dyn. 236:791.
  16. Iwata, J. et al. (2012) J. Clin. Invest. 122:873.
  17. Gatza, C.E. et al. (2010) Cell. Signal. 22:1163.

Long Name

Transforming Growth Factor beta 3

Alternate Names

ARVD1, LDS5, RNHF, TGFB3, TGFbeta 3

Entrez Gene IDs

7043 (Human); 21809 (Mouse); 25717 (Rat)

Gene Symbol

TGFB3

UniProt

Product Documents for Recombinant Human TGF-beta 3 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TGF-beta 3 Protein

For research use only

Citations for Recombinant Human TGF-beta 3 Protein (117)

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FAQs for Recombinant Human TGF-beta 3 Protein

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  • Q: Is Recombinant Human TGF-beta 3 Protein (Catalog # 243-B3) latent or active?

    A: The sequence for this protein is Spodoptera frugiperda, Sf 21 (baculovirus)-derived human TGF-beta 3 protein, Ala301-Ser412 (Tyr340Phe), Accession # P10600. Ala301-Ser412 is associated with the active form of the protein and does not include the latency-associated peptide sequence (amino acids 24-300).

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Reconstitution Calculator

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