TGF-beta 3 Biotinylated Antibody

Name: TGF-beta 3 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF243
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF243

Citations (2)

(1)

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Key Product Details

Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant chicken TGF‑ beta 3 and recombinant human TGF‑ beta 3
Applications:	ELISA Detection
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects TGF-beta 3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) TGF‑ beta 1, rhTGF-beta 1.2, and rhTGF-beta 2 is observed.

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Applications for TGF-beta 3 Biotinylated Antibody

Human TGF-beta 3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: TGF‑ beta 3 Antibody (Catalog # MAB643)

Standard: Recombinant Human TGF-beta 3 Protein (Catalog # 243-B3)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 3

TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1 - 7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1 - 4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8 - 10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity.(1) TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription ( 3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

Item	Value
References	Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982. Wahl, S.M. (2006) Immunol. Rev. 213:213. Chang, H. et al. (2002) Endocr. Rev. 23:787. Lin, J.S. et al. (2006) Reproduction 132:179. Hinck, A.P. et al. (1996) Biochemistry 35:8517. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261. Derynck, R. et al. (1988) EMBO J. 7:3737. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name	Transforming Growth Factor beta 3
Entrez Gene IDs	7043 (Human); 21809 (Mouse); 25717 (Rat)
Alternate Names	ARVD, ARVD1, FLJ16571, LDS5, RNHF, TGF-beta 3, TGF-beta-3, TGF-beta3, TGFB3, TGFbeta 3, transforming growth factor beta-3, transforming growth factor, beta 3

Citations for TGF-beta 3 Biotinylated Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for TGF-beta 3 Biotinylated Antibody include:

Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Liu X et al. (2002), Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am. J. Physiol. Lung Cell Mol. Physiol., 282(5):L1049-56.
PMID: 11943670
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development

Simpson H et al. (2002), Transforming growth factor beta expression in human placenta and placental bed during early pregnancy. 6897, 23(1):44-58.
PMID: 11869091

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TGF-beta 3 Biotinylated Antibody

Name: Anonymous

Sample Tested: Recombinant protein

Species: Human

Verified Customer | Posted 03/28/2019

Direct ELISA to confirm specificity using recombinant protein

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Product Documents for TGF-beta 3 Biotinylated Antibody

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TGF-beta 3 Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for TGF-beta 3 Biotinylated Antibody

Human TGF-beta 3 Sandwich Immunoassay

Formulation, Preparation and Storage

Reconstitution Calculator

Background: TGF-beta 3

Citations for TGF-beta 3 Biotinylated Antibody (2)

Customer Reviews for TGF-beta 3 Biotinylated Antibody (1)

Customer Images

TGF-beta 3 Biotinylated Antibody

Product Documents for TGF-beta 3 Biotinylated Antibody

Product Datasheets

COA

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