PARP Antibody (194C1439) [DyLight 650] - Cleaved
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27335C
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat (Negative)
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
DyLight 650 (Excitation = 652 nm, Emission = 672 nm)
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 194C1439
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP.
Specificity
This monoclonal antibody specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for PARP Antibody (194C1439) [DyLight 650] - Cleaved
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-27335. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
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Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: PARP
PARP is inactivated by caspase cleavage, leading to a programmed cell death pathway.
Long Name
Poly [ADP-ribose] Polymerase
Alternate Names
ADPRT, PARP1, PPOL
Gene Symbol
PARP1
Additional PARP Products
Product Documents for PARP Antibody (194C1439) [DyLight 650] - Cleaved
Product Specific Notices for PARP Antibody (194C1439) [DyLight 650] - Cleaved
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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