PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27335
![Western Blot: PARP Antibody (194C1439)CleavedAzide and BSA Free [NBP2-27335]](https://resources.bio-techne.com/images/products/PARP-Antibody-194C1439-Azide-Free-Cleaved-Western-Blot-NBP2-27335-img0003.jpg "Western Blot: PARP Antibody (194C1439)CleavedAzide and BSA Free [NBP2-27335]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat (Negative)
Cited:
Human
Applications
Validated:
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 194C1439
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP.
Specificity
This monoclonal antibody specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
Western Blot: PARP Antibody (194C1439)CleavedAzide and BSA Free [NBP2-27335]
Western Blot: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, using this antibody. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.![Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved - Azide and BSA Free [NBP2-27335]](https://resources.bio-techne.com/images/products/PARP-Antibody-194C1439-Azide-Free-Cleaved-Immunocytochemistry-Immunofluorescence-NBP2-27335-img0004.jpg "Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved - Azide and BSA Free [NBP2-27335]")
Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved - Azide and BSA Free [NBP2-27335]
Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Western Blot: PARP Antibody (194C1439)CleavedAzide and BSA Free [NBP2-27335]](https://resources.bio-techne.com/images/products/PARP-Antibody-194C1439-Azide-Free-Western-Blot-NBP2-27335-img0002.jpg "Western Blot: PARP Antibody (194C1439)CleavedAzide and BSA Free [NBP2-27335]")
Western Blot: PARP Antibody (194C1439)CleavedAzide and BSA Free [NBP2-27335]
Western Blot: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - Analysis using 293T lysates expressing PARP. Image from verified customer review.Applications for PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1ul/1 million cells
Immunocytochemistry/ Immunofluorescence
2-5 ug/ml
Western Blot
1-2ug/ml~. Use reported in scientific literature (PMID 27470599)
Application Notes
This antibody is CyTOF ready.
Reviewed Applications
Read 1 review rated 5 using NBP2-27335 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PARP
Long Name
Poly [ADP-ribose] Polymerase
Alternate Names
ADPRT, PARP1, PPOL
Gene Symbol
PARP1
Additional PARP Products
Product Documents for PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
Product Specific Notices for PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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