Mouse PD-L1/B7-H1 Antibody

Name: Mouse PD-L1/B7-H1 Antibody
Brand: R&D Systems
SKU: AF1019
Rating: 4 (10 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1019

Citations (26)

(10)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse PD-L1/B7-H1 Phe19-Thr238 Accession # Q9EP73
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse PD-L1/B7-H1 in direct ELISAs and Western blots.

View Full Technical Details for Mouse PD-L1/B7-H1 Antibody

Applications for Mouse PD-L1/B7-H1 Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Flow Cytometry	0.25 µg/10⁶ cells	See below
Immunohistochemistry	5-15 µg/mL	Perfusion fixed frozen sections of mouse small intestine (Peyer's patch) and thymus
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse PD-L1/B7-H1 Antibody

	Western Blot Detection of Mouse PD-L1/B7-H1 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Flow Cytometry Detection of PD-L1/B7-H1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open histogram) was stained with Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
	Flow Cytometry Detection of PD-L1/B7-H1 in HEK293 Human Cell Line Transfected with Mouse PD-L1/B7-H1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse PD-L1/B7-H1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: PD-L1/B7-H1

Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1‑4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20‑40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium (1‑4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-gamma treatment induces B7‑H1/PD‑L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also been reported (1‑4).

Item	Value
References	Tamura, H. et al. (2001) Blood 97:1809. Freeman, G. et al. (2000) J. Exp. Med. 192:1027. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Long Name	Programmed Death Ligand 1
Entrez Gene IDs	29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names	Avelumab, B7-H, B7-H1, B7H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PD-L1, PD-L1B7 homolog 1, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PDL1PDCD1 ligand 1, Programmed death ligand 1, programmed cell death 1 ligand 1

Citations for Mouse PD-L1/B7-H1 Antibody (26)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse PD-L1/B7-H1 Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

N Ishikura et al. (2022), Anti?VEGF antibody triggers the effect of anti?PD?L1 antibody in PD?L1low and immune desert?like mouse tumors Oncology reports, 47(2).
PMID: 34958105
Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA

Y Li et al. (2022), A novel multifunctional anti-PD-L1-CD16a-IL15 induces potent cancer cell killing in PD-L1-positive tumour cells Translational Oncology, 21(0):101424.
PMID: 35477065
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

TJ Barley et al. (2022), Mitogen-Activated Protein Kinase Phosphatase-1 Controls PD-L1 Expression by Regulating Type I Interferon during Systemic Escherichia coli Infection The Journal of Biological Chemistry, 0(0):101938.
PMID: 35429501
TN Pham et al. (2022), Inhibiting MNK kinases promotes macrophage immunosuppressive phenotype to limit CD8+ T cell anti-tumor immunity JCI Insight, 0(0).
PMID: 35380995
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

J Jin et al. (2022), PD-L1 near Infrared Photoimmunotherapy of Ovarian Cancer Model Cancers, 14(3).
PMID: 35158887
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Z Shi et al. (2021), Loss of H2R signaling disrupts neutrophil homeostasis and promotes inflammation-associated colonic tumorigenesis in mice Cellular and Molecular Gastroenterology and Hepatology, 0(0).
PMID: 34781022
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

J Kim et al. (2021), A Novel Kinase Inhibitor AX-0085 Inhibits Interferon-gamma-Mediated Induction of PD-L1 Expression and Promotes Immune Reaction to Lung Adenocarcinoma Cells Cells, 11(1).
PMID: 35011581
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Y Philippou et al. (2020), Impacts of combining anti-PD-L1 immunotherapy and radiotherapy on the tumour immune microenvironment in a murine prostate cancer model Br. J. Cancer, 0(0).
PMID: 32641865
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

T Lerbs et al. (2020), CD47 prevents the elimination of diseased fibroblasts in scleroderma JCI Insight, 5(16).
PMID: 32814713
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

RA Fernandes et al. (2020), Immune receptor inhibition through enforced phosphatase recruitment Nature, 586(7831):779-784.
PMID: 33087934
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

T Lerbs et al. (2020), CD47 prevents the elimination of diseased fibroblasts in scleroderma JCI Insight, 5(16).
PMID: 32814713
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

F Ritzmann et al. (2019), IL-17C-mediated innate inflammation decreases the response to PD-1 blockade in a model of Kras-driven lung cancer Sci Rep, 9(1):10353.
PMID: 31316109
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

LM Schreiber et al. (2019), The lytic activity of VSV-GP treatment dominates the therapeutic effects in a syngeneic model of lung cancer Br. J. Cancer, 0(0).
PMID: 31530903
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

P Srinivasan et al. (2018), PD-L1 checkpoint inhibition and anti-CTLA-4 whole tumor cell vaccination counter adaptive immune resistance: A mouse neuroblastoma model that mimics human disease PLoS Med., 15(1):e1002497.
PMID: 29377881
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

KC Osum et al. (2018), Interferon-gamma drives programmed death-ligand 1 expression on islet ? cells to limit T cell function during autoimmune diabetes Sci Rep, 8(1):8295.
PMID: 29844327
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

V Dimitrov et al. (2017), Hormonal vitamin D upregulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in human but not mouse J. Biol. Chem., 0(0).
PMID: 29061851
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

MY Bartee et al. (2017), Tumor localized secretion of soluble PD1 enhances oncolytic virotherapy Cancer Res, 0(0).
PMID: 28314785
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

SJ Sung et al. (2017), Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice J. Immunol, 0(0).
PMID: 28219886
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

(2016), PD-1/PD-L1 blockade enhances T cell activity and antitumor efficacy of imatinib in gastrointestinal stromal tumors Clin Cancer Res, 0(0).
PMID: 27470968
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

(2016), Cell autonomous or systemic EGFR blockade alters the immune-environment in squamous cell carcinomas Int J Cancer, 0(0).
PMID: 27509256
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

El Khatib M et al. (2015), beta-Cell-targeted blockage of PD1 and CTLA4 pathways prevents development of autoimmune diabetes and acute allogeneic islets rejection. Gene Ther, 22(5):430-8.
PMID: 25786871
Abiko K et al. (2015), IFN-gamma from lymphocytes induces PD-L1 expression and promotes progression of ovarian cancer. Br J Cancer, 112(9):1501-9.
PMID: 25867264
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Duncan F et al. (2013), Endogenous retinoids in the pathogenesis of alopecia areata. J Invest Dermatol, 133(2):334-43.
PMID: 23014334
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Kasagi S et al. (2010), Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice. J. Immunol., 184(5):2337-47.
PMID: 20139271
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Yadav D et al. (2009), Altered availability of PD-1/PD ligands is associated with the failure to control autoimmunity in NOD mice. Cell. Immunol., 258(2):161-71.
PMID: 19497561
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Wang J et al. (2005), Establishment of NOD-Pdcd1-/- mice as an efficient animal model of type I diabetes. Proc. Natl. Acad. Sci. U.S.A., 102(33):11823-8.
PMID: 16087865

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Customer Reviews for Mouse PD-L1/B7-H1 Antibody (10)

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Mouse PD-L1 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Breast cancer tissue

Species: Mouse

Verified Customer | Posted 07/31/2020
Mouse PD-L1 Antibody

Name: Shubhankar Ambike

Application: Flow

Sample Tested: MH-S mouse macrophage cell line,RAW 264.7 mouse monocyte/macrophage cell line

Species: Mouse

Verified Customer | Posted 01/21/2020

MH-S and RAW264.7 cells were treated with 10ug/ml concentration of IFN alpha. Cells were analyzed by flow cytometer (surface staining) for PD-L1 expression using a labelled secondary antibody. Protocol was followed as given with the article. Worked exactly as expected.
Mouse PD-L1 Antibody

Name: Shubhankar Ambike

Application: WB

Sample Tested: Cell Lysates

Species: Mouse

Verified Customer | Posted 12/31/2019

MH-S mice macrophage cell lysates. Dark bands in the middle are PD-L1
Mouse PD-L1 Antibody

Name: Anonymous

Application: WB

Sample Tested: HCT-116 human colorectal carcinoma cell line

Species: Human

Verified Customer | Posted 03/20/2019
Mouse PD-L1 Antibody

Name: Dong Hu

Application: WB

Sample Tested: mouse beast cancer cell line Eo771

Species: Mouse

Verified Customer | Posted 02/28/2019
Mouse PD-L1 Antibody

Name: Anonymous

Application: WB

Sample Tested: 4T1 mouse breast cancer cell line,EMT6 cells

Species: Mouse

Verified Customer | Posted 10/28/2018
Mouse PD-L1/B7-H1 Antibody

Name: Anonymous

Application: IHC

Sample Tested: frozen section

Species: Mouse

Verified Customer | Posted 03/22/2018
Mouse PD-L1/B7-H1 Antibody

Name: Angelo Taglialatela

Application: WB

Sample Tested: cultured mouse mammary gland tumor,mouse mammary gland tumor cells

Species: Mouse

Verified Customer | Posted 11/26/2017

Technical Service will be following up with the customer.
Mouse PD-L1/B7-H1 Antibody

Name: Purushottam Lamichhane

Application: IHC

Sample Tested: Pancreas tissue

Species: Mouse

Verified Customer | Posted 10/17/2017
Mouse B7‑H1/PD‑L1 Antibody

Name: Anonymous

Application: WB

Sample Tested: Mouse lymphocytes

Species: Mouse

Verified Customer | Posted 10/26/2015

Buffer: tbstDilution: 1/1000

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Mouse PD-L1/B7-H1 Antibody

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Applications for Mouse PD-L1/B7-H1 Antibody

Scientific Data Examples for Mouse PD-L1/B7-H1 Antibody

Western Blot

Flow Cytometry

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: PD-L1/B7-H1

Citations for Mouse PD-L1/B7-H1 Antibody (26)

Customer Reviews for Mouse PD-L1/B7-H1 Antibody (10)

Customer Images

Mouse PD-L1 Antibody

Mouse PD-L1 Antibody

Mouse PD-L1 Antibody

Mouse PD-L1 Antibody

Mouse PD-L1 Antibody

Mouse PD-L1 Antibody

Mouse PD-L1/B7-H1 Antibody

Mouse PD-L1/B7-H1 Antibody

Mouse PD-L1/B7-H1 Antibody

Mouse B7‑H1/PD‑L1 Antibody

Product Documents for Mouse PD-L1/B7-H1 Antibody

Product Datasheets

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