Mouse IL-6R alpha Antibody

Name: Mouse IL-6R alpha Antibody
Brand: R&D Systems
SKU: AF1830

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1830

Citations (15)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse IL-6R alpha Leu20-Glu357 Accession # P22272
Applications:	Block/Neutralize, CyTOF-ready, ELISA Capture, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse IL-6R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant mouse (rm) IL‑6, recombinant human IL-6 R and rmgp130 is observed.

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Applications for Mouse IL-6R alpha Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL-6R alpha (Catalog # 1830-SR)
Flow Cytometry	2.5 µg/10⁶ cells	Mouse CD3⁺ splenocytes
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Mouse IL-6R alpha Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Mouse IL-6R alpha Biotinylated Antibody (Catalog # BAF1830)

Standard: Recombinant Mouse IL-6R alpha (aa 20-357) Protein (Catalog # 1830-SR)

Item	Value
Neutralization	Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R. P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND₅₀) is typically 0.2-1 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse IL-6R alpha Antibody

	Immunohistochemistry IL-6R alpha in Mouse Thymus. IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Immunohistochemistry IL-6R alpha in Mouse Thymus. IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL-6R alpha Antibody. Recombinant Mouse IL-6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830). The ND₅₀ is typically 0.2-1 µg/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-6R alpha

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

Item	Value
References	Yamasaki, K. et al. (1988) Science 241:825. Sugita, T. et al. (1990) J. Exp. Med. 171:2001. Hibi, M. et al. (1990) Cell 63:1149. Saito, M. et al. (1992) J. Immunol. 148:4066. Novick, D. et al. (1989) J. Exp. Med. 170:1409. Jones, S.A. et al. (2001) FASEB J. 15:43. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.
Long Name	Interleukin 6 Receptor alpha
Entrez Gene IDs	3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names	CD126, IL-6 R alpha, IL-6R alpha, IL-6R-1, IL-6Ra, IL6Q, IL6R, IL6R alpha, IL6RA, IL6RQ, gp80, interleukin 6 receptor

Citations for Mouse IL-6R alpha Antibody (15)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-6R alpha Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Shimada et al. (2021), Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice Toxicology reports, 8(0):520-528.
PMID: 33747797
Species: Human
Sample Types: Whole Tissue
Applications: IHC

D Zhao et al. (2020), Chromatin Regulator, CHD1, Remodels the Immunosuppressive Tumor Microenvironment in PTEN-Deficient Prostate Cancer Cancer Discov, 0(0).
PMID: 32385075
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

M Zhou et al. (2020), Extracellular CIRP induces macrophage endotoxin tolerance through IL-6R-mediated STAT3 activation JCI Insight, 0(0).
PMID: 32027619
Species: Human
Sample Types: Protein Lysates
Applications: Western Blot

H Huang et al. (2019), Targeting TGFbetaR2-mutant tumors exposes vulnerabilities to stromal TGFbeta blockade in pancreatic cancer EMBO Mol Med, 0(0):e10515.
PMID: 31609088
Species: Mouse
Sample Types: Transduced Whole Cells
Applications: Neutralization

SM Kim et al. (2019), Role of Proinflammatory Cytokines in Feedback Modulation of Circadian Clock Gene Rhythms by Saturated Fatty Acids Sci Rep, 9(1):8909.
PMID: 31222133
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

AJ St Leger et al. (2018), STAT-3-independent production of IL-17 by mouse innate-like ?? T cells controls ocular infection J. Exp. Med., 0(0).
PMID: 29490936
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

X Wang et al. (2017), Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130 J Neuroinflammation, 14(1):121.
PMID: 28645275
P Arnold et al. (2017), Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling Sci Rep, 7(0):44053.
PMID: 28276471
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Nana Owusu-Boai (2016), Macrophagic control of the response to uropathogenic E. coli infection by regulation of iron retention in an IL-6-dependent manner Immun Inflamm Dis, 4(4):413-426.
PMID: 27980776
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot

Garbers C et al. (2011), Species Specificity of ADAM10 and ADAM17 Proteins in Interleukin-6 (IL-6) Trans-signaling and Novel Role of ADAM10 in Inducible IL-6 Receptor Shedding. J. Biol. Chem., 286(17):14804-11.
PMID: 21454673
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot

Walker F et al. (2007), IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice. Blood, 111(8):3978-85.
PMID: 18156493
Species: Mouse
Sample Types: Air Pouch Exudate
Applications: ELISA Development

Rabe B et al. (2007), Transgenic blockade of interleukin 6 transsignaling abrogates inflammation. Blood, 111(3):1021-8.
PMID: 17989316
Species: Mouse
Sample Types: Lavage Fluid
Applications: ELISA Development

Chalaris A et al. (2007), Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils. Blood, 110(6):1748-55.
PMID: 17567983
Species: Mouse

Applications: Neutralization

Kurooka M et al. (2007), Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells. Cancer Res., 67(1):227-36.
PMID: 17210703
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development

Osuchowski MF et al. (2005), Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 302(1):172-81.
PMID: 16023134

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