Mouse IL-6R alpha Biotinylated Antibody

Name: Mouse IL-6R alpha Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1830

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF1830

Citations (3)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse IL-6R alpha Leu20-Glu357 Accession # P22272
Applications:	ELISA Detection, Flow Cytometry, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse IL-6R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 1% cross-reactivity with recombinant human IL‑6R, recombinant mouse (rm) IL‑6, and rmgp130 is observed.

View Full Technical Details for Mouse IL-6R alpha Biotinylated Antibody

Complete Your Research

Applications for Mouse IL-6R alpha Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL-6R alpha (Catalog # 1830-SR)
Flow Cytometry	2.5 µg/10⁶ cells	Mouse CD3⁺ splenocytes

Mouse IL-6R alpha Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Standard: Recombinant Mouse IL-6R alpha (aa 20-357) Protein (Catalog # 1830-SR)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-6R alpha

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1-4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51%, and 50% aa identity with rat, human, and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

Item	Value
References	Yamasaki, K. et al. (1988) Science 241:825. Sugita, T. et al. (1990) J. Exp. Med. 171:2001. Hibi, M. et al. (1990) Cell 63:1149. Saito, M. et al. (1992) J. Immunol. 148:4066. Novick, D. et al. (1989) J. Exp. Med. 170:1409. Jones, S.A. et al. (2001) FASEB J. 15:43. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.
Long Name	Interleukin 6 Receptor alpha
Entrez Gene IDs	3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names	CD126, IL-6 R alpha, IL-6R alpha, IL-6R-1, IL-6Ra, IL6Q, IL6R, IL6R alpha, IL6RA, IL6RQ, gp80, interleukin 6 receptor

Citations for Mouse IL-6R alpha Biotinylated Antibody (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-6R alpha Biotinylated Antibody include:

Species: Mouse
Sample Types: Air Pouch Exudate

Rabe B et al. (2007), Transgenic blockade of interleukin 6 transsignaling abrogates inflammation. Blood, 111(3):1021-8.
PMID: 17989316
Species: Mouse
Sample Types: Lavage Fluid
Applications: ELISA Development

Chalaris A et al. (2007), Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils. Blood, 110(6):1748-55.
PMID: 17567983
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development

Osuchowski MF et al. (2005), Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 302(1):172-81.
PMID: 16023134

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Product Documents for Mouse IL-6R alpha Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS