Mouse IL-17C Antibody

Name: Mouse IL-17C Antibody
Brand: R&D Systems
SKU: MAB23061

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB23061

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Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse IL‑17C His15-Gln194 Accession # NP_665833
Applications:	CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 311522
Specificity:	Detects mouse IL-17C in direct ELISAs.

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Applications for Mouse IL-17C Antibody

	Recommended Concentration	Sample
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse IL-17C Antibody

	Intracellular Staining by Flow Cytometry Detection of IL‑17C in Tramp‑C1 Mouse Cell Line by Flow Cytometry. Tramp‑C1 mouse prostate cancer cell line was stained with Rat Anti-Mouse IL‑17C Monoclonal Antibody (Catalog # MAB23061, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-17C

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and
IL‑17 RE, have also been reported (1‑4). The functions of IL-17 RC, D, and E are not known.

Mouse IL-17C cDNA encodes a 194 amino acid (aa) residues protein with a putative 14 aa signal peptide (5). Although there are no potential N-linked glycosylation sites, it is reportedly glycosylated (6). IL-17C shares from 15%‑30% aa sequence identity with other IL-17 family members. Mouse and human IL-17C share 83% aa sequence identity. IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) in an adult prostate and fetal kidney libraries (2). IL-17C has been shown to stimulate the release of TNF-alpha and IL-1 beta from the monocytic cell line THP-1, a property it shares with IL-17B (6, 7). Human IL‑17C is active on mouse cells (5). The receptor of IL-17C has not yet been identified. The IL-17C preparations from R&D Systems have been found to bind immobilized recombinant mouse IL-17B R/Fc in a functional ELISA.

Item	Value
References	Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309. Hurst, S.D. et al. (2002) J. Immunology 169:443. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
Long Name	Interleukin 17C
Entrez Gene IDs	27189 (Human); 234836 (Mouse)
Alternate Names	CX2, Cytokine CX2, IL-17C, IL-17CMGC126884, IL17C, MGC138401, interleukin 17C, interleukin-17C

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Mouse IL-17C Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Mouse IL-17C Antibody

Scientific Data Examples for Mouse IL-17C Antibody

Intracellular Staining by Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-17C

Citations for Mouse IL-17C Antibody

Product Documents for Mouse IL-17C Antibody

Product Datasheets

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