Mouse gp130 Antibody

Name: Mouse gp130 Antibody
Brand: R&D Systems
SKU: MAB4681

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB4681

Citations (7)

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Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse gp130 Gln23-Glu617 (predicted) Accession # Q6PDI9
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 125623
Specificity:	Detects mouse gp130 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human gp130 is observed.

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Applications for Mouse gp130 Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse gp130 Antibody

	Flow Cytometry Detection of gp130 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4681) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
	Immunocytochemistry gp130 in M1 Mouse Cell Line. gp130 was detected in immersion fixed M1 mouse myeloid leukemia cell line using Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4681) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013)and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

Item	Value
References	Narazaki, M. et al. (1993) Blood 82:1120. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name	Glycoprotein 130
Entrez Gene IDs	3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names	CD130, CD130 antigen, CDw130, DKFZp564F053, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, IL6ST, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form, interleukin 6 signal transducer (gp130, oncostatin M receptor), interleukin receptor beta chain, interleukin-6 receptor subunit beta, membrane glycoprotein gp130

Citations for Mouse gp130 Antibody (7)

Showing 1 - 7 of 7 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse gp130 Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

AN Wilkinson et al. (2018), Granulocytes Are Unresponsive to IL-6 Due to an Absence of gp130 J. Immunol., 0(0).
PMID: 29626088
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

C Li et al. (2017), Dendritic cell MST1 inhibits Th17 differentiation Nat Commun, 8(0):14275.
PMID: 28145433
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

S Chehboun et al. (2017), Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling J. Biol. Chem, 0(0).
PMID: 28280243
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Le Goff B et al. (2014), Oncostatin M acting via OSMR, augments the actions of IL-1 and TNF in synovial fibroblasts. Cytokine, 68(2):101-9.
PMID: 24767864
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Bailey et al. (2012), Ifitm3 limits the severity of acute influenza in mice. PLoS Pathog, 8(9):e1002909.
PMID: 22969429
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Matsumoto S et al. (2010), Essential roles of IL-6 trans-signaling in colonic epithelial cells, induced by the IL-6/soluble-IL-6 receptor derived from lamina propria macrophages, on the development of colitis-associated premalignant cancer in a murine model. J. Immunol., 184(3):1543-51.
PMID: 20042582
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot

(2007), TH and NPY in sympathetic neurovascular cultures: role of LIF and NT-3. Am. J. Physiol., Cell Physiol., 294(1):C306-12.
PMID: 18032527

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Showing 1 - 7 of 7 citations Showing All

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Mouse gp130 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse gp130 Antibody

Scientific Data Examples for Mouse gp130 Antibody

Flow Cytometry

Immunocytochemistry

Formulation, Preparation and Storage

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Background: gp130

Citations for Mouse gp130 Antibody (7)

Product Documents for Mouse gp130 Antibody

Product Datasheets

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