Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody

Name: Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody
Brand: R&D Systems
SKU: F0113

R&D Systems, Inc. a Bio-Techne Brand

Catalog # F0113

Citations (3)

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Key Product Details

Species Reactivity:	Rat
Immunogen:	Rat IgG heavy and light chains
Applications:	Flow Cytometry
Label:	Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source:	Polyclonal Goat IgG
Specificity:	Detects rat IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate mouse cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')₂ fragments which have reduced interactions with cell surface Fc receptors.

View Full Technical Details for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Applications for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, wherethe primary antibody does not have a fluorescent reporter, is of rat origin, and is of IgG class. The recommendedconcentration is 10 μL/10⁶ cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

	Flow Cytometry Detection of Rat IgG Primary Antibody by Flow Cytometry bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB5994, filled histogram) or isotype control antibody (Catalog # MAB006), open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) at 10 µL/10⁶cells.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Item	Value
Long Name	Immunoglobulin G
Alternate Names	IgG, Immunoglobulin G, ImmunoglobulinG

Citations for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

J Choi et al. (2019), Glycolipid Stimulation of Invariant NKT Cells Expands a Unique Tissue-Resident Population of Precursors to Mature NK Cells Endowed with Oncolytic and Antimetastatic Properties J. Immunol., 0(0).
PMID: 31462506
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Goury A et al. (2015), Deletion of receptor for advanced glycation end products exacerbates lymphoproliferative syndrome and lupus nephritis in B6-MRL Fas lpr/j mice. J Immunol, 194(8):3612-22.
PMID: 25762779
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Lundqvist A et al. (2009), Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells. Blood, 113(24):6120-7.
PMID: 19202127

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Product Documents for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Scientific Data Examples for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: IgG

Citations for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody (3)

Product Documents for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody

Scientific Data Examples for Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: IgG

Citations for Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody (3)

Product Documents for Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Applications for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Scientific Data Examples for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Citations for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody (3)

Product Documents for Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody