Mouse Endoglin/CD105 Antibody

Name: Mouse Endoglin/CD105 Antibody
Brand: R&D Systems
SKU: AF1320
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1320

Citations (18)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105 Glu27-Gly581 Accession # Q8K100
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse Endoglin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant rat Endoglin is observed and less than 5% cross-reactivity with recombinant human Endoglin is observed.

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Applications for Mouse Endoglin/CD105 Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Flow Cytometry	2.5 µg/10⁶ cells	MS-1 mouse cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse Endoglin/CD105 Antibody

	Western Blot Detection of Mouse Endoglin/CD105 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line and MS-1 mouse pancreatic islet endothelial cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endoglin/CD105 at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry Endoglin/CD105 in MS-1 Mouse Cell Line. Endoglin/CD105 was detected in immersion fixed MS-1 mouse pancreatic islet endothelial cell line using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunocytochemistry Endoglin/CD105 in Rat Mesenchymal Stem Cells. Endoglin/CD105 was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry Endoglin/CD105 in Mouse Embryo. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (E13-15) using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Simple Western Detection of Mouse Endoglin/CD105 by Simple Western^TM. Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Endoglin/CD105 at approximately 121 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

Item	Value
References	Ge, A.Z. and E.C. Butcher (1994) Gene 138:201. ten Dijke, P. et al. (2008) Angiogenesis 11:79. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375. Mancini, M.L. et al. (2007) Dev. Biol. 308:520. Moody, J.L. et al. (2007) Stem Cells 25:2809. Velasco, S. et al. (2008) J. Cell Sci. 121:913. Perez-Gomez, E. et al. (2005) Oncogene 24:4450. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800. Arthur, H.M. et al. (2000) Dev. Biol. 217:42. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs	2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names	CD105, CD105 antigen, ENDOsler-Rendu-Weber syndrome 1, ENG, Endoglin, HHT1FLJ41744, ORW, ORW1

Citations for Mouse Endoglin/CD105 Antibody (18)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Endoglin/CD105 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

S Magalhaes- et al. (2022), Mitochondrial respiration supports autophagy to provide stress resistance during quiescence Autophagy, 0(0):1-18.
PMID: 35258392
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

DD He et al. (2020), C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors Stem Cell Reports, 0(0).
PMID: 32220331
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

N García-Rom et al. (2020), Bevacizumab dose adjustment to improve clinical outcomes of glioblastoma BMC Med, 18(1):142.
PMID: 32564774
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Y Yoon et al. (2020), PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival Mol. Psychiatry, 0(0).
PMID: 32541930
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC-P

M Ishikawa et al. (2019), Correlation of two distinct metastasis-associated proteins, MTA1 and S100A4, in angiogenesis for promoting tumor growth Oncogene, 0(0).
PMID: 30745574
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

R Bauer et al. (2018), Blockade of myeloid-derived suppressor cell expansion with all-trans retinoic acid increases the efficacy of anti-angiogenic therapy Cancer Res., 0(0).
PMID: 29674477
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

DL Coutu et al. (2018), Multicolor quantitative confocal imaging cytometry Nat. Methods, 15(1):39-46.
PMID: 29320487
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

G Eelen et al. (2018), Role of glutamine synthetase in angiogenesis beyond glutamine synthesis Nature, 561(7721):63-69.
PMID: 30158707
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

EM Palmieri et al. (2017), Pharmacologic or Genetic Targeting of Glutamine Synthetase Skews Macrophages toward an M1-like Phenotype and Inhibits Tumor Metastasis Cell Rep, 20(7):1654-1666.
PMID: 28813676
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

N García-Rom et al. (2017), DNA sequences within glioma-derived extracellular vesicles can cross the intact blood-brain barrier and be detected in peripheral blood of patients Oncotarget, 8(1):1416-1428.
PMID: 27902458
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

M Wroblewski et al. (2017), Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B Nat Commun, 8(1):269.
PMID: 28814715
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

(2016), PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia Nat Commun, 7(0):13650.
PMID: 27897192
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Stimac M et al. (2015), Gene Electrotransfer of Plasmid with Tissue Specific Promoter Encoding shRNA against Endoglin Exerts Antitumor Efficacy against Murine TS/A Tumors by Vascular Targeted Effects. PLoS ONE, 10(4):e0124913.
PMID: 25909447
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Grafton K et al. (2014), LF-15 & T7, synthetic peptides derived from tumstatin, attenuate aspects of airway remodelling in a murine model of chronic OVA-induced allergic airway disease. PLoS ONE, 9(1):e85655.
PMID: 24454912
Species: Human
Sample Types: Whole Cells
Applications: IHC

Dolinsek T et al. (2013), Multiple delivery of siRNA against endoglin into murine mammary adenocarcinoma prevents angiogenesis and delays tumor growth. PLoS ONE, 8(3):e58723.
PMID: 23593103
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Jimenez V et al. (2013), In vivo adeno-associated viral vector-mediated genetic engineering of white and brown adipose tissue in adult mice. Diabetes, 62(12):4012-22.
PMID: 24043756
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Park S et al. (2013), Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments. J Immunol, 190(3):1094-102.
PMID: 23264658
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Lin FJ et al. (2010), Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J. Clin. Invest., 120(5):1694-707.
PMID: 20364082

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Mouse Endoglin/CD105 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Embryonic heart

Species: Mouse

Verified Customer | Posted 05/16/2017

Block - 5% secondary specific serum + 1% BSA + 0.5% Tween-20 - 2hrs at RTPrimary Ab - diluted in block - 1 in 25 - for O/N at 4'CSecondary ab - diluted in block - 2hrs at RT

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Mouse Endoglin/CD105 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Mouse Endoglin/CD105 Antibody

Scientific Data Examples for Mouse Endoglin/CD105 Antibody

Western Blot

Immunocytochemistry

Immunocytochemistry

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Endoglin/CD105

Citations for Mouse Endoglin/CD105 Antibody (18)

Customer Reviews for Mouse Endoglin/CD105 Antibody (1)

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Mouse Endoglin/CD105 Antibody

Product Documents for Mouse Endoglin/CD105 Antibody

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