Human Thrombospondin-1 Antibody

Name: Human Thrombospondin-1 Antibody
Brand: R&D Systems
SKU: AF3074
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF3074

Citations (7)

(3)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-1 Asn19-Pro1170 Accession # CAA32889
Applications:	Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Thrombospondin-1 in direct ELISAs and Western blots.

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Applications for Human Thrombospondin-1 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Thrombospondin-1 Antibody

	Western Blot Detection of Human Thrombospondin‑1 by Western Blot. Western blot shows lysates of MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Thrombospondin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Thrombospondin-1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry Thrombospondin‑1 in Human Colon. Thrombospondin-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Thrombospondin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Simple Western Detection of Human Thrombospondin‑1 by Simple Western^TM. Simple Western lane view shows lysates of MBA-MB-468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin-1 at approximately 221 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Thrombospondin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Thrombospondin-1

Thrombospondin-1 (TSP-1) is a 150‑180 kDa charter member of the thrombospondin family of extracellular matrix proteins. Human TSP‑1 is synthesized as an 1170 amino acid (aa) precursor that contains an 18 aa signal sequence and 1152 aa mature molecule. The mature molecule has been described as containing three distinct regions that create the shape of a dumbbell. There is an intital, 140 aa N-terminal laminin G-like globular region that binds heparin (aa 19‑258). This is followed by an extended, central collagen-binding region that contains one type C von Willebrand factor domain, plus three TSP type I and three TSP type II (or EGF-like) domains (aa 259‑712). The C‑terminus (aa 713‑1170) appears as a large globule with two halves; one calcium‑binding region (aa 713‑950) with seven Asp‑rich TSP type III domains, and one terminal region (aa 951‑1170) with TSP‑unique motifs (1). This C-terminal region is believed to mediate CD47 and cell binding (2‑5). The TSP type I repeats have multiple functions. They bind to type V collagen, laminin, fibronectin and CD36. They also contain a recognition site for C‑mannosylation on Trp. Finally, a type I KRFK motif induces the release of mature TGF-b from LAP. This is an effect not found in TSP-2. The function of the type II repeats in unclear. TSP‑1 is secreted as a disulfide-linked 450 kDa homotrimer. The cysteines responsible lie just N‑terminal to the first type I TSP repeat. Mature human TSP-1 is 61% aa identical to human TSP-2. It is also 95%, 97% and 95% aa identical to mouse, dog and rat TSP-1, respectively.

Item	Value
References	Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635. Frazier, W.A. (1987) J. Cell Biol. 105:625. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961. Sid, B. et al. (2004) Crit. Rev. Oncol. Hematol. 49:245. Floquet, N. et al. (2008) Anch. Biochem. Biophys. 478:103.
Entrez Gene IDs	7057 (Human); 21825 (Mouse)
Alternate Names	THBS, THBS-1, THBS1, TSP-1, TSP1thrombospondin-1, TSPTSP-1, Thrombospondin-1, Thrombospondin1, thrombospondin 1, thrombospondin-1p180

Citations for Human Thrombospondin-1 Antibody (7)

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Citations are publications that use Bio-Techne products. Selected citations for Human Thrombospondin-1 Antibody include:

Species: Mouse
Sample Types: Tissue Homogenates
Applications: IHC

D Vanhoutte et al. (2021), Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy Nature Communications, 12(1):3928.
PMID: 34168130
Species: Human
Sample Types: Cell Culture Supernates
Applications: Dot Blot

GK Annarapu et al. (2021), Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion Redox Biology, 48(0):102205.
PMID: 34891098
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Bhattachar et al. (2020), Oncogenes overexpressed in metastatic oral cancers from patients with pain: potential pain mediators released in exosomes Sci Rep, 10(1):14724.
PMID: 32895418
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

CD Wiley et al. (2019), SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells Cell Rep, 28(13):3329-3337.e5.
PMID: 31553904
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P

S Koh et al. (2018), Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates Mï¿½ller Glial Reactivity J. Neurosci., 0(0).
PMID: 29431645
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

X Miao et al. (2018), Thrombin-reduced miR-27b attenuates platelet angiogenic activities in vitro via enhancing platelet synthesis of anti-angiogenic thrombospondin-1 J. Thromb. Haemost., 0(0).
PMID: 29442415
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

Villarreal G et al. (2015), Canonical wnt signaling regulates extracellular matrix expression in the trabecular meshwork. Invest Ophthalmol Vis Sci, 55(11):7433-40.
PMID: 25352117

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Customer Reviews for Human Thrombospondin-1 Antibody (3)

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Human Thrombospondin-1 Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Serum and Plasma

Species: Human

Verified Customer | Posted 10/04/2019

We used this antibody for a sandwich ELISA in combination with mAb (MAB3074)) and protein (3074-TH). This combination works very well for detecting the TSP1 in human serum and plasma samples.
Human Thrombospondin-1 Antibody

Name: Anonymous

Application: WB

Sample Tested: Trabecular meshwork

Species: Human

Verified Customer | Posted 01/16/2018
Human Thrombospondin-1 Antibody

Name: Juliana Redondo

Application: WB

Sample Tested: Mesenchymal stem cells

Species: Human

Verified Customer | Posted 05/20/2016

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Human Thrombospondin-1 Antibody

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Applications for Human Thrombospondin-1 Antibody

Scientific Data Examples for Human Thrombospondin-1 Antibody

Western Blot

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Thrombospondin-1

Citations for Human Thrombospondin-1 Antibody (7)

Customer Reviews for Human Thrombospondin-1 Antibody (3)

Customer Images

Human Thrombospondin-1 Antibody

Human Thrombospondin-1 Antibody

Human Thrombospondin-1 Antibody

Product Documents for Human Thrombospondin-1 Antibody

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