Human Thrombospondin-1 Antibody

Name: Human Thrombospondin-1 Antibody
Brand: R&D Systems
SKU: MAB3074
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB3074

Citations (3)

(1)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-1 Asn19-Pro1170 Accession # CAA32889
Applications:	ELISA Capture, Immunoprecipitation, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 301221
Specificity:	Detects human Thrombospondin‑1 in ELISAs and Western blots. In sandwich immunoassays and Western blots, no cross‑reactivity with recombinant human (rh) Thrombospindin-2 or rhThrombospondin-4 is observed.

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Applications for Human Thrombospondin-1 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human Thrombospondin‑1 (Catalog # 3074-TH) under non-reducing conditions only
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Human Thrombospondin‑1 (Catalog # 3074-TH), see our available Western blot detection antibodies

Human Thrombospondin-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Thrombospondin‑1 Biotinylated Antibody (Catalog # BAF3074)

Standard: Recombinant Human Thrombospondin-1 Protein, CF (Catalog # 3074-TH)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombospondin-1

Thrombospondin-1 (TSP-1) is a 150‑180 kDa charter member of the thrombospondin family of extracellular matrix proteins. Human TSP‑1 is synthesized as an 1170 amino acid (aa) precursor that contains an 18 aa signal sequence and 1152 aa mature molecule. The mature molecule has been described as containing three distinct regions that create the shape of a dumbbell. There is an intital, 140 aa N-terminal laminin G-like globular region that binds heparin (aa 19‑258). This is followed by an extended, central collagen-binding region that contains one type C von Willebrand factor domain, plus three TSP type I and three TSP type II (or EGF-like) domains (aa 259‑712). The C‑terminus (aa 713‑1170) appears as a large globule with two halves; one calcium‑binding region (aa 713‑950) with seven Asp‑rich TSP type III domains, and one terminal region (aa 951‑1170) with TSP‑unique motifs (1). This C-terminal region is believed to mediate CD47 and cell binding (2‑5). The TSP type I repeats have multiple functions. They bind to type V collagen, laminin, fibronectin and CD36. They also contain a recognition site for C‑mannosylation on Trp. Finally, a type I KRFK motif induces the release of mature TGF-b from LAP. This is an effect not found in TSP-2. The function of the type II repeats in unclear. TSP‑1 is secreted as a disulfide-linked 450 kDa homotrimer. The cysteines responsible lie just N‑terminal to the first type I TSP repeat. Mature human TSP-1 is 61% aa identical to human TSP-2. It is also 95%, 97% and 95% aa identical to mouse, dog and rat TSP-1, respectively.

Item	Value
References	Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635. Frazier, W.A. (1987) J. Cell Biol. 105:625. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961. Sid, B. et al. (2004) Crit. Rev. Oncol. Hematol. 49:245. Floquet, N. et al. (2008) Anch. Biochem. Biophys. 478:103.
Entrez Gene IDs	7057 (Human); 21825 (Mouse)
Alternate Names	THBS, THBS-1, THBS1, TSP-1, TSP1thrombospondin-1, TSPTSP-1, Thrombospondin-1, Thrombospondin1, thrombospondin 1, thrombospondin-1p180

Citations for Human Thrombospondin-1 Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Thrombospondin-1 Antibody include:

Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture)

K Endt et al. (2017), Development and clinical testing of individual immunoassays for the quantification of serum glycoproteins to diagnose prostate cancer PLoS ONE, 12(8):e0181557.
PMID: 28767721
Species: Human
Sample Types: Whole Cells
Applications: ICC

WH Kahr et al. (2017), Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease Nat Commun, 8(0):14816.
PMID: 28368018
Species: Human
Sample Types: Cell Lysates

Peterson JE et al. (2010), Normal ranges of angiogenesis regulatory proteins in human platelets. Am. J. Hematol., 85(7):487-93.
PMID: 20575035

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Customer Reviews for Human Thrombospondin-1 Antibody (1)

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Human Thrombospondin-1 Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Serum and Plasma

Species: Human

Verified Customer | Posted 10/04/2019

We used this antibody for a sandwich ELISA in combination with pAb (AF3074)) and protein (3074-TH). This combination works very well for detecting the TSP1 in human serum and plasma samples.

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Human Thrombospondin-1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human Thrombospondin-1 Antibody

Human Thrombospondin-1 Sandwich Immunoassay

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Thrombospondin-1

Citations for Human Thrombospondin-1 Antibody (3)

Customer Reviews for Human Thrombospondin-1 Antibody (1)

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Human Thrombospondin-1 Antibody

Product Documents for Human Thrombospondin-1 Antibody

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