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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PR7212

Product Summary for Human PDGF R beta Antibody

Immunogen

Human skin fibroblast membrane extracts

Specificity

Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF receptor beta‑subunit complexed with 125I‑PDGF-BB (3). It does not recognize the PDGF receptor alpha‑subunit. Detects the PDGF receptor beta‑subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PDGF R beta Antibody

Detection of PDGF R beta in MG-63 cells by Flow Cytometry

MG-63 cells were stained with Mouse Anti-Human PDGF R beta Monoclonal Antibody (Catalog # MAB1263, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PDGF R beta Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: MG-63 human osteosarcoma cell line

Immunoprecipitation

Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.

Western Blot

1 µg/mL
Sample: Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4 using MAB1263 in the following applications:

Published Applications

Read 17 publications using MAB1263 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immuno‑globulinlike domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist (4).

References

  1. Hart et al. (1987) J. Biol. Chem. 262:10780.
  2. Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.
  3. Seifert et al. (1989) J. Biol. Chem. 264:8771.
  4. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

Long Name

Platelet-derived Growth Factor Receptor beta

Alternate Names

CD140b, PDGFRB

Entrez Gene IDs

5159 (Human); 18596 (Mouse); 24629 (Rat)

Gene Symbol

PDGFRB

Product Documents for Human PDGF R beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PDGF R beta Antibody

For research use only

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