Human PD‑1 Antibody
Catalog # MAB10864 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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Product Summary for Human PD‑1 Antibody
Immunogen
Leu25-Thr168
Accession # Q15116
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human PD‑1 Antibody
Detection of Human PD‑1 by Western Blot.
Western blot shows lysates of human tonsil tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10864) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PD-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.PD‑1 in Human Tonsil.
PD-1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human PD-1 Monoclonal Antibody (Catalog # MAB10864) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.PD-L1/B7-H1 Binding to PD-1 Blocked by Human PD-1 Antibody.
In a functional ELISA, 0.09-0.72 µg/mL of this antibody (green line) will block 50% of the binding of 5 µg/mL of Recombinant Human PD-L1/B7-H1 Fc Chimera (orange line, Catalog # 156-B7) to immobilized Recombinant Human PD-1 His-tagged Protein (Catalog # 8986-PD) coated at 1 µg/mL (100 µL/well). At 5 µg/mL, this antibody will block >90% of the binding.Applications for Human PD‑1 Antibody
Blockade of Receptor-ligand Interaction
ELISA
This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human PD‑1 Monoclonal Antibody (Catalog # MAB10863).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PD-1 DuoSet ELISA Kit (Catalog # DY1086) for convenient development of a sandwich ELISA.
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Western Blot
Sample: Human Tonsil Tissue
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-1
References
- Ishida, Y. et al. (1992) EMBO J. 11:3887.
- Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
- Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
- Watanabe, N et al. (2003) Nat. Immunol. 4:670.
- Zhang, X. et al. (2004) Immunity 20:337.
- Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483.
- Nishimura, H et al. (1996) Int. Immunol. 8:773.
- Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677.
- Butte, M.J. et al. (2007) Immunity 27:111.
- Okazaki, T. et al. (2013) Nat. Immunol. 14:1212.
- Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 12293.
- Nogrady, B. (2014) Nature 513:S10.
- Swaika, A. et al. (2015) Mol. Immunol. 67: 4
Long Name
Alternate Names
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UniProt
Product Documents for Human PD‑1 Antibody
Product Specific Notices for Human PD‑1 Antibody
For research use only
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.