Human Pax6 Antibody

Name: Human Pax6 Antibody
Brand: R&D Systems
SKU: AF8150
Rating: 4 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF8150

Citations (10)

(3)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human Pax6 Met1-Arg272 Accession # P26367
Applications:	Flow Cytometry, Immunocytochemistry/Immunofluorescence, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Sheep IgG
Specificity:	Detects human Pax6 in direct ELISAs.

View Full Technical Details for Human Pax6 Antibody

Applications for Human Pax6 Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Pax6 Antibody

	Western Blot Detection of Human and Rat Pax6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and rat cortical stem cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Pax6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8150) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pax6 at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry Pax6 in SA01 Human Embryonic Stem Cells. Immersion fixed SA01 human embryonic stem cells were differentiated for 6 days with Recombinant Human Noggin (Catalog # 6057-NG) and SB431542 (upper panel) or differentiated for 6 days with Recombinant Human BMP-4 (negative control, lower panel; Catalog # 314-BP). Pax6 was detected using Sheep Anti-Human Pax6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8150) at 5 µg/mL. Cells were stained using an Alexa Fluor 488-conjugated Anti-Sheep IgG Secondary Antibody (green) and counterstained with DAPI (blue). Specific staining was localized to nuclei.Images courtesy of Dr. Ron McKay, Leiber Institute for Brain Development, Baltimore, Maryland, USA.
	Intracellular Staining by Flow Cytometry Detection of Pax6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Pax6 Affinity-Purified Polyclonal Antibody (Catalog # AF8150, filled histogram) or Sheep IgG control Antibody (Catalog # 5-001-A, open histogram) followed by anti-Sheep IgG PE-conjugated Secondary Antibody (Catalog # F0126). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
	Simple Western Detection of Human Pax6 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Pax6 at approximately 59 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human Pax6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8150) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Pax6

Pax6 (paired box 6; also Oculorhombin) is a 48‑50 kDa member of the paired homeobox family of transcription factors. It is expressed in developing optic vesicle, olfactory dopaminergic neurons, and pancreatic endocrine cells. Pax6 is a transactivating protein that interacts with MAF, CDX2 and SOX2. Human Pax6 is 422 amino acids (aa) in length. It contains an N-terminal paired box DNA-binding domain (aa 4‑130), a Gly-rich central region (aa 131‑209), a homeodomain (aa 213‑269) and a C-terminal Pro/Ser/Thr-rich regulatory domain (aa 279‑422). Phosphorylation of the C‑terminal domain at Thr281/304/373 promotes Pax6 activity. Multiple splice forms of Pax6 exist. There are alternative start sites at Met137 and a position 34 aa upstream of the standard site. There is also a deletion of aa 22‑26 and 37‑39, plus a 14 aa insertion after Gln47 that generates a C‑terminal DNA binding site. Human and mouse Pax6 are absolutely identical in aa sequence.

Item	Value
Long Name	Paired Box Gene 6
Entrez Gene IDs	5080 (Human); 18508 (Mouse); 25509 (Rat)
Alternate Names	MGC17209, Oculorhombin, Pax6, keratitis), paired box 6, paired box protein Pax-6

Citations for Human Pax6 Antibody (10)

Showing 1 - 10 of 10 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human Pax6 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

HS Meharena et al. (2022-01-01), Down-syndrome-induced senescence disrupts the nuclear architecture of neural progenitors Cell Stem Cell, 29(1):116-130.e7.
PMID: 34995493
Species: Mice
Sample Types: Whole Tissue
Applications: IHC

X Dong et al. (2022-01-01), Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex Nature Neuroscience, 0(0).
PMID: 35726058
Species: Human
Sample Types: Organoid
Applications: IHC

V Krenn et al. (2021-01-01), Organoid modeling of Zika and herpes simplex virus 1 infections reveals virus-specific responses leading to microcephaly Cell Stem Cell, 0(0).
PMID: 33838105
Species: Human
Sample Types: Whole Tissue
Applications: IHC

AZ Eriksen et al. (2021-01-01), SARS-CoV-2 infects human adult donor eyes and hESC-derived ocular epithelium Cell Stem Cell, 0(0).
PMID: 34022129
Species: Human
Sample Types: Whole Tissue
Applications: IHC

M Shibata et al. (2021-01-01), Regulation of prefrontal patterning and connectivity by retinoic acid Nature, 0(0).
PMID: 34599305
Species: Human
Sample Types: Whole Cells
Applications: ICC

SR Sripathy et al. (2020-01-01), Generation of 10 patient-specific induced pluripotent stem cells (iPSCs) to model Pitt-Hopkins Syndrome Stem Cell Res, 48(0):102001.
PMID: 32971458
Species: Human
Sample Types: Whole Cells
Applications: ICC

C Fan et al. (2020-01-01), TALEN-mediated biallelic inactivation of MYB in human embryonic stem cell lines WAe001-A-45 and WAe001-A-46 Stem Cell Res, 46(0):101854.
PMID: 32526676
Species: Human
Sample Types: Whole Cells
Applications: ICC

X Gao et al. (2018-01-01), Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel Stem Cell Res, 31(0):193-196.
PMID: 30099336
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

WA Pastor et al. (2018-01-01), TFAP2C regulates transcription in human naive pluripotency by opening enhancers Nat. Cell Biol., 20(5):553-564.
PMID: 29695788
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

A Filippello et al. (2018-01-01), Chronic Exposure to Palmitate Impairs Insulin Signaling in an Intestinal L-cell Line: A Possible Shift from GLP-1 to Glucagon Production Int J Mol Sci, 19(12).
PMID: 30487448

There are no citations that match your criteria.

Showing 1 - 10 of 10 citations Showing All

Customer Reviews for Human Pax6 Antibody (3)

4.3 out of 5

3 customer ratings

5 stars

33%

4 stars

67%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 3 of 3 reviews Showing All

Filter By:

Human Pax6 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Neural progenitor cells

Species: Human

Verified Customer | Posted 09/26/2018

Pax6 was detected human embryonic stem cells-derived neural stem cells using Sheep Anti-Human PAX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8150) at 1 µg/mL for overnight at 4°C. Cells were stained using the Donkey anti-sheep IgG (H+L) Cross-Adsorbed, Alexa Fluor® 568.
Human Pax6 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Cerebellum tissue

Species: Mouse

Verified Customer | Posted 11/02/2017

Works fine at 1/1000 dilution. O.N. incubation at 4C.
Human Pax6 Antibody

Name: Juan Zanin

Application: IHC

Sample Tested: Cerebellum tissue

Species: Mouse

Verified Customer | Posted 07/13/2016

There are no reviews that match your criteria.

Showing 1 - 3 of 3 reviews Showing All

Product Documents for Human Pax6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Human Pax6 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Human Pax6 Antibody

Scientific Data Examples for Human Pax6 Antibody

Western Blot

Immunocytochemistry

Intracellular Staining by Flow Cytometry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Pax6

Citations for Human Pax6 Antibody (10)

Customer Reviews for Human Pax6 Antibody (3)

Customer Images

Human Pax6 Antibody

Human Pax6 Antibody

Human Pax6 Antibody

Product Documents for Human Pax6 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected