Human Pax6 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC8150P

Catalog #
Availability
Size / Price
Qty
IC8150P-100
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Sheep IgG

View all Pax6 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pax6
Met1-Arg272
Accession # P26367

Specificity

Detects human Pax6 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Pax1, rhPax2, rhPax3, rhPax4, rhPax5, and rhPax7 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Pax6 PE-conjugated Antibody

Detection of Pax6 in Jurkat cells by Flow Cytometry.

Jurkat cells were stained with Sheep Anti-Human Pax6 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC8150P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human Pax6 PE-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample:
Jurkat human acute T cell leukemia cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pax6

Pax6 (paired box 6; also Oculorhombin) is a 48-50 kDa member of the paired homeobox family of transcription factors.  It is expressed in developing optic vesicle, olfactory dopaminergic neurons, and pancreatic endocrine cells.  Pax6 is a transactivating protein that interacts with MAF, CDX2 and SOX2.  Human Pax6 is 422 amino acids (aa) in length.  It contains an N-terminal paired box DNA-binding domain (aa 4-130), a Gly-rich central region (aa 131-209), a homeodomain (aa 213-269) and a C-terminal Pro/Ser/Thr-rich regulatory domain (aa 279-422).  Phosphorylation of the C-terminal domain at Thr281/304/373 promotes Pax6 activity.  Multiple splice forms of Pax6 exist. There are alternative start sites at Met137 and a position 34 aa upstream of the standard site.  There is also a deletion of aa 22-26 and 37‑39, plus a 14 aa insertion after Gln47 that generates a C-terminal DNA binding site.  Human and mouse Pax6 are absolutely identical in aa sequence.

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Entrez Gene IDs

5080 (Human); 18508 (Mouse); 25509 (Rat)

Gene Symbol

PAX6

UniProt

P26367

Additional Pax6 Products

Product Documents for Human Pax6 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Pax6 PE-conjugated Antibody

For research use only

Privacy Policy
Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.