Human/Mouse Neogenin Antibody

Name: Human/Mouse Neogenin Antibody
Brand: R&D Systems
SKU: AF1079
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1079

Citations (12)

(1)

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Key Product Details

Species Reactivity:	Human, Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse Neogenin Ala42-Ile1033 (Asp442-Leu461 del) Accession # NP_032710
Applications:	Block/Neutralize, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human and mouse Neogenin in direct ELISAs and Western blots.

View Full Technical Details for Human/Mouse Neogenin Antibody

Applications for Human/Mouse Neogenin Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 2.5-10 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Chicken Netrin-1 (Catalog # 128‑N1) to immobilized Recombinant Mouse Neogenin Fc Chimera (Catalog # 1079‑NE) coated at 5 µg/mL (100 µL/well). At 300 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse Neogenin Antibody

	Western Blot Detection of Human and Mouse Neogenin by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line, Jurkat human acute T cell leukemia cell line, and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neogenin at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry Neogenin in Mouse Embryo. Neogenin was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Simple Western Detection of Mouse Neogenin by Simple Western^TM. Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neogenin at approximately 207 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Neogenin

Neogenin (NEO) is a type I transmembrane protein that is crucial for axonal guidance and neuronal migration. It is also involved in regulating differentiation programs in many embryonic and adult tissues (1). Mouse NEO is widely expressed in adult tissues and is expressed throughout the mid to late stages of gestation, in both neuronal and non-neuronal tissues. It is a member of the immunoglobulin (Ig) superfamily and is closely related to deleted in colorectal cancer (DCC). Mouse NEO cDNA encodes a 1493 amino acid residue (aa) precursor with a putative 36 aa signal peptide, a 1100 aa extracellular domain with six Ig-like C2 type domains and three fibronectin type III domains, a 21 aa transmembrane domain, and a 345 aa cytoplasmic domain. At least five isoforms are produced in mice by alternative splicing. Mouse NEO shares 96%, 93%, and 86% aa sequence identity with rat, human, and chicken NEO, respectively. It also has 46% and 29% sequence homology with mouse DCC and C. elegans UNC40, a homolog of DCC. NEO and DCC, together with the UNC5 family of type I transmembrane proteins, are receptors for the netrin/UNC6 family of laminin-related bifunctional guidance molecules that both attract some axons and repel others (2, 3). In mouse, at least five netrins (netrin‑1,
‑3, -4, G1, and G2) have been identified (3-5). Mouse netrin-1 and netrin-3 have been shown to be ligands for mouse NEO.

Item	Value
References	Keeling, S.L. et al. (1997) Oncogene 15:691. Hong, K. et al. (1999) Cell 97:927. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62. Nakashiba, T. et al. (2000) J. Neurosci. 20:6540. Nakashiba, T. et al. (2002) Mech. Dev. 111:47.
Entrez Gene IDs	4756 (Human); 18007 (Mouse)
Alternate Names	HsT17534, IGDCC2DKFZp547B146, Immunoglobulin superfamily DCC subclass member 2, NEO, NEO1, NGN, NGNDKFZp547A066, Neogenin, neogenin (chicken) homolog 1, neogenin 1, neogenin homolog 1

Product Specific Notices for Human/Mouse Neogenin Antibody

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.

Citations for Human/Mouse Neogenin Antibody (12)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Neogenin Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

G Untiveros et al. (2022), Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells International Journal of Molecular Sciences, 23(21).
PMID: 36361539
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

N Apóstolo et al. (2020), Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 11(1):5171.
PMID: 33057002
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

J Liu et al. (2018), Tbr1 instructs laminar patterning of retinal ganglion cell dendrites Nat. Neurosci., 0(0).
PMID: 29632360
Species: Human
Sample Types: Whole Cells
Applications: Apoptosis

M Schlegel et al. (2018), Inhibition of neogenin fosters resolution of inflammation and tissue regeneration J. Clin. Invest., 0(0).
PMID: 30222138
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

T Hadi et al. (2018), Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells Nat Commun, 9(1):5022.
PMID: 30479344
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Frydlova J et al. (2015), Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats. PLoS ONE, 11(2):e0148540.
PMID: 26845567
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Konig K et al. (2012), The axonal guidance receptor neogenin promotes acute inflammation. PLoS ONE, 7(3):e32145.
PMID: 22412855
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Palmesino E et al. (2012), Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebrates. J. Neurosci., 32(2):411-6.
PMID: 22238077
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Wang W et al. (2009), Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis. Am. J. Pathol., 175(3):1010-8.
PMID: 19700747
Species: Chicken
Sample Types: Tissue Homogenates
Applications: Western Blot

Osterfield M et al. (2008), Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system. Development, 135(6):1189-99.
PMID: 18272596
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Neutralization

Kuns-Hashimoto R et al. (2008), Selective binding of RGMc/hemojuvelin, a key protein in systemic iron metabolism, to BMP-2 and neogenin. Am. J. Physiol., Cell Physiol., 294(4):C994-C1003.
PMID: 18287331
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Lejmi E et al. (2008), Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B. Proc. Natl. Acad. Sci. U.S.A., 105(34):12491-6.
PMID: 18719102

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Customer Reviews for Human/Mouse Neogenin Antibody (1)

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Human/Mouse Neogenin Antibody

Name: Anonymous

Application: WB

Sample Tested: Brain tissue

Species: Mouse

Verified Customer | Posted 01/25/2019

Dilution used for the western is 1:250

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Human/Mouse Neogenin Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human/Mouse Neogenin Antibody

Scientific Data Examples for Human/Mouse Neogenin Antibody

Western Blot

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Neogenin

Product Specific Notices for Human/Mouse Neogenin Antibody

Citations for Human/Mouse Neogenin Antibody (12)

Customer Reviews for Human/Mouse Neogenin Antibody (1)

Customer Images

Human/Mouse Neogenin Antibody

Product Documents for Human/Mouse Neogenin Antibody

Product Datasheets

COA

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SDS

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