Recombinant Mouse Neogenin Fc Chimera Protein, CF

Name: Recombinant Mouse Neogenin Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1079-NE

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1079-NE

Citations (1)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	NP_032710
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Mouse Neogenin Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse Neogenin protein

Mouse Neogenin (Ala42-Ile1033) (Asp442-Leu461 del) Accession # NP_032710	IEGRID	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala42

Predicted Molecular Mass

133 kDa (monomer)

SDS-PAGE

155-175 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Neogenin Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Chicken Netrin‑1 (Catalog # 128-N1) with a linear range of 6-400 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1079-NE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neogenin

Neogenin is a type I transmembrane protein belonging to the Ig superfamily. It is composed of an extracellular segment containing four Ig-like C2-type domains and six Fibronectin type III domains (1). Neogenin has a molecular weight of approximately 190 kDa, and the extracellular domain of the mouse protein shares 91% and 94% amino acid sequence identity with the human and rat orthologs, respectively (1). Five different isoforms are produced from alternative splicing of mouse Neo1. Neogenin is widely expressed in both neuronal and non-neuronal tissues of the developing mouse embryo, and in most tissues in adult mice (2). It is a multifunctional cell-surface receptor that binds to members of the Netrin, Repulsive Guidance Molecule (RGM) and Bone Morphogenetic Protein (BMP) families (3-5). It has also been shown to interact with members of the UNC5 family and in certain instances, associate with CDO as a co-receptor (6-8). Neogenin appears to be involved in the regulation of multiple developmental processes including development of the central nervous system (CNS), myogenesis, angiogenesis, and formation of mammary glands (4, 5, 7-9). During CNS development, Neogenin regulates neural tube closure, neuronal differentiation, and cell survival (4, 5, 7). It also mediates
Netrin-1-dependent attraction and RGM-A-dependent repulsion of growing axons (4, 5, 7, 10). Additionally, Neogenin binding to RGM and Netrin proteins regulates cell-cell adhesion, cell migration, tissue organization, and adult neurogenesis (4, 7, 11). Neogenin is thought to be involved in tumorgenesis and cancer cell invasiveness in brain and gastric cancers (12-14).

Item	Value
References	Meyerhardt, J.A. et al. (1997) Oncogene 14:1129. Keeling, S.L. et al. (1997) Oncogene 15:691. Hagihara, M. et al. (2011) J. Biol. Chem. 286:5157. Tian, C. and J. Liu (2013) Mol. Reprod. Dev. 80:700. Severyn, C.J. et al. (2009) Biochem. J. 422:393. van den Heuvel, D.M. et al. (2013) PLoS ONE 8:e55828. De Vries, M. and H.M. Cooper (2008) J. Neurochem. 106:1483. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355. Srinivasan, K. et al. (2003) Dev. Cell 4:371. de Castro, F. (2003) News Physiol. Sci. 18:130. O’Leary, C.J. et al. (2015) Stem Cells 33:503. Milla, L.A. et al. (2014) Int. J. Cancer 134:21. Akino, T. et al. (2014) Cancer Res. 74:3716. Kim, S.J. et al. (2014) Oncotarget 5:3386.
Entrez Gene IDs	4756 (Human); 18007 (Mouse)
Alternate Names	HsT17534, IGDCC2DKFZp547B146, Immunoglobulin superfamily DCC subclass member 2, NEO, NEO1, NGN, NGNDKFZp547A066, Neogenin, neogenin (chicken) homolog 1, neogenin 1, neogenin homolog 1

Citations for Recombinant Mouse Neogenin Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Neogenin Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: ELISA (Capture)

Lejmi E et al. (2008), Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B. Proc. Natl. Acad. Sci. U.S.A., 105(34):12491-6.
PMID: 18719102

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