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Recombinant Mouse Neogenin Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1079-NE

Catalog #
Size / Price

Key Product Details



Accession #

Structure / Form

Disulfide-linked homodimer




Binding Activity

Product Specifications


Mouse myeloma cell line, NS0-derived mouse Neogenin protein
Mouse Neogenin
(Asp442-Leu461 del)
Accession # NP_032710
N-terminus C-terminus


>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

133 kDa (monomer)


155-175 kDa, reducing conditions


Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse Neogenin Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Chicken Netrin‑1 (Catalog # 128-N1) with a linear range of 6-400 ng/mL.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neogenin

Neogenin is a type I transmembrane protein belonging to the Ig superfamily. It is composed of an extracellular segment containing four Ig-like C2-type domains and six Fibronectin type III domains (1). Neogenin has a molecular weight of approximately 190 kDa, and the extracellular domain of the mouse protein shares 91% and 94% amino acid sequence identity with the human and rat orthologs, respectively (1). Five different isoforms are produced from alternative splicing of mouse Neo1. Neogenin is widely expressed in both neuronal and non-neuronal tissues of the developing mouse embryo, and in most tissues in adult mice (2). It is a multifunctional cell-surface receptor that binds to members of the Netrin, Repulsive Guidance Molecule (RGM) and Bone Morphogenetic Protein (BMP) families (3-5). It has also been shown to interact with members of the UNC5 family and in certain instances, associate with CDO as a co-receptor (6-8). Neogenin appears to be involved in the regulation of multiple developmental processes including development of the central nervous system (CNS), myogenesis, angiogenesis, and formation of mammary glands (4, 5, 7-9). During CNS development, Neogenin regulates neural tube closure, neuronal differentiation, and cell survival (4, 5, 7). It also mediates
Netrin-1-dependent attraction and RGM-A-dependent repulsion of growing axons (4, 5, 7, 10). Additionally, Neogenin binding to RGM and Netrin proteins regulates cell-cell adhesion, cell migration, tissue organization, and adult neurogenesis (4, 7, 11). Neogenin is thought to be involved in tumorgenesis and cancer cell invasiveness in brain and gastric cancers (12-14).


  1. Meyerhardt, J.A. et al. (1997) Oncogene 14:1129.
  2. Keeling, S.L. et al. (1997) Oncogene 15:691.
  3. Hagihara, M. et al. (2011) J. Biol. Chem. 286:5157.
  4. Tian, C. and J. Liu (2013) Mol. Reprod. Dev. 80:700.
  5. Severyn, C.J. et al. (2009) Biochem. J. 422:393.
  6. van den Heuvel, D.M. et al. (2013) PLoS ONE 8:e55828.
  7. De Vries, M. and H.M. Cooper (2008) J. Neurochem. 106:1483.
  8. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355.
  9. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  10. de Castro, F. (2003) News Physiol. Sci. 18:130.
  11. O’Leary, C.J. et al. (2015) Stem Cells 33:503.
  12. Milla, L.A. et al. (2014) Int. J. Cancer 134:21.
  13. Akino, T. et al. (2014) Cancer Res. 74:3716.
  14. Kim, S.J. et al. (2014) Oncotarget 5:3386.

Alternate Names


Entrez Gene IDs

4756 (Human); 18007 (Mouse)

Gene Symbol



Additional Neogenin Products

Product Documents for Recombinant Mouse Neogenin Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Neogenin Fc Chimera Protein, CF

For research use only