Recombinant Chicken Netrin-1 Protein

Name: Recombinant Chicken Netrin-1 Protein
Brand: R&D Systems
SKU: 128-N1
Price: 428 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 128-N1

Citations (8)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q90922
Applications:	Binding Activity

View Full Technical Details for Recombinant Chicken Netrin-1 Protein

Product Specifications for Recombinant Chicken Netrin-1 Protein

Source

Mouse myeloma cell line, NS0-derived chicken Netrin-1 protein

Chicken Netrin-1 (Gly26 - Ala606) Accession # Q90922	DIEGRGGGSGGGSGGGS	10-His tag
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly26

Predicted Molecular Mass

66.3 kDa

SDS-PAGE

75-85 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rcNetrin-1 with a linear range of 6-400 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

128-N1

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

128-N1/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Netrin-1

Chicken Netrin-1 is the prototypical member of an ever-expanding, laminin-related family of axon-guidance molecules collectively referrred to as netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 606 amino acid (aa) protein precursor that has structural similarity to the N-terminus of the B2 or gamma -chain of laminin. It contains one 250 aa type VI globular domain, three type V (~55 aa) cysteine/glycine rich EGF repeats, and one unique 140 aa “C” domain that binds heparin. Chick Netrin-1 shares 78% aa identity with chicken Netrin-2 and 86% aa identity with mouse and human Netrin-1. Although only two chick netrins are known, the number of known mammalian netrins is increasing. Human and mouse Netrin-3/NTN-2L, and a mouse Netrin-4, that shares homology with the B1 or beta -chain of laminin, have been reported. Cells reported to express Netrin-1 in the embryo include cells of the spinal cord floor plate and somite, cells of the ganglionic eminence, and cells of the floor plate of the met- and caudal mesencephalon. In the adult, neurons of the thalamus, neocortex, and hippocampus, plus Schwann cells, osteoclasts and osteoblasts all reportedly produce Netrin-1. The DCC (deleted in colorectal cancer) gene product as well as the UNC5 family of receptors and the adenosine A2b receptor have been proposed to be functional receptors for Netrin-1.

Item	Value
References	Serafini, T. et al. (1994) Cell 78:409. Kennedy, T.E. et al. (1994) Cell 78:425. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287. MacLennan, A.J. et al. (1997) J. Neurosci. 17:5466. Koch, M. et al. (2000) J. Cell. Biol. 151:221. Corset, V. et al. (2000) Nature 407:747.
Entrez Gene IDs	9423 (Human); 18208 (Mouse); 396389 (Chicken)
Alternate Names	NTN1, NTN1Lnetrin 1, mouse, homolog of, Netrin-1, Netrin1, netrin 1

Citations for Recombinant Chicken Netrin-1 Protein (8)

Showing 1 - 8 of 8 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Chicken Netrin-1 Protein include:

Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay

CY Huang et al. (2017), K(+) channel Kv3.4 is essential for axon growth by limiting the influx of Ca(2+) into growth cones J. Neurosci, 0(0).
PMID: 28320840
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

(2016), CD151 mediates netrin-1-induced angiogenesis through the Src-FAK-Paxillin pathway J Cell Mol Med, 0(0).
PMID: 27558487
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

Li J et al. (2014), Evidence for topographic guidance of dopaminergic axons by differential Netrin-1 expression in the striatum. Mol Cell Neurosci, 61(0):85-96.
PMID: 24867253
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

van Gils et al. (2013), Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 33(5):911-9.
PMID: 23430612
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lejmi E et al. (2008), Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B. Proc. Natl. Acad. Sci. U.S.A., 105(34):12491-6.
PMID: 18719102
Species: Hamster
Sample Types: Whole Cells
Applications: Bioassay

Hutchins BI et al. (2008), Differential outgrowth of axons and their branches is regulated by localized calcium transients. J. Neurosci., 28(1):143-53.
PMID: 18171932
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ly NP et al. (2005), Netrin-1 inhibits leukocyte migration in vitro and in vivo. Proc. Natl. Acad. Sci. U.S.A., 102(41):14729-34.
PMID: 16203981
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

Tsai HH et al. (2003), Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal. Development, 130(10):2095-105.
PMID: 12668624

Recombinant Chicken Netrin-1 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Chicken Netrin-1 Protein

Formulation, Preparation and Storage

Carrier Free

128-N1

Carrier

128-N1/CF

Carrier Free

Reconstitution Calculator

Background: Netrin-1

Citations for Recombinant Chicken Netrin-1 Protein (8)

Product Documents for Recombinant Chicken Netrin-1 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected