Human/Mouse HIF-1 alpha/HIF1A PE‑conjugated Antibody
Catalog # IC1935P | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Human/Mouse HIF-1 alpha/HIF1A PE‑conjugated Antibody
Immunogen
Arg575-Asn826
Accession # Q16665.1
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse HIF-1 alpha/HIF1A PE‑conjugated Antibody
Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line treated with CoCl2was stained with Mouse Anti-Human/Mouse HIF-1a PE-conjugated Monoclonal Antibody (Catalog # IC1935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse HIF-1 alpha/HIF1A PE‑conjugated Antibody
Intracellular Staining by Flow Cytometry
Sample: MCF‑7 human breast cancer cell line, treated with CoCl2, fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation and Storage
Purification
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Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HIF-1 alpha/HIF1A
The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
Product Documents for Human/Mouse HIF-1 alpha/HIF1A PE‑conjugated Antibody
Product Specific Notices for Human/Mouse HIF-1 alpha/HIF1A PE‑conjugated Antibody
For research use only
Citations for Human/Mouse HIF-1 alpha/HIF1A PE‑conjugated Antibody (8)
Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse HIF-1 alpha/HIF1A PE‑conjugated Antibody include:
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Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryJH Park et al. (2022-11-08), Obesity enhances antiviral immunity in the genital mucosa through a microbiota-mediated effect on gammadelta T�cells Cell Reports, 2022-141(6):111594.
PMID: 36351403 -
Species: Human
Sample Types: Whole Cells
Applications: ICCY Sun et al. (2021-12-22), Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells Science Advances, 2021-17(52):eabj6877.
PMID: 34936448 -
Species: Human,Mouse
Sample Types: Whole Cells
Applications: Flow CytometryS Becker-Her et al. (2021-03-04), CD74 is a regulator of hematopoietic stem cell maintenance PloS Biology, 2021-019(3):e3001121.
PMID: 33661886 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryM Okada et al. (2020-11-09), Epithelial-mesenchymal transition and metastatic ability of CD133+ colorectal cancer stem-like cells under hypoxia Oncol Lett, 2020-121(1):19.
PMID: 33240425 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryE Khatib-Mas et al. (2020-07-15), Lactate released by inflammatory bone marrow neutrophils induces their mobilization via endothelial GPR81 signaling Nat Commun, 2020-011(1):3547.
PMID: 32669546 -
Species: Human
Sample Types: Cells
Applications: Flow CytometryLS Sellam et al. (2020-07-15), Silibinin down-regulates PD-L1 expression in nasopharyngeal carcinoma by interfering with tumor cell glycolytic metabolism Arch. Biochem. Biophys., 2020-0690(0):108479.
PMID: 32679194 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryG Duette et al. (2018-09-11), Induction of HIF-1? by HIV-1 Infection in CD4+ T Cells Promotes Viral Replication and Drives Extracellular Vesicle-Mediated Inflammation MBio, 2018-09(5).
PMID: 30206166 -
Species: Mouse
Sample Types: Cell Lysates,Whole Cells
Applications: Flow Cytometry,Western BlotY Lu et al. (2017-03-13), Glucocorticoid receptor promotes the function of myeloid-derived suppressor cells by suppressing HIF1?-dependent glycolysis Cell. Mol. Immunol, 2017-00(0).
PMID: 28287112
There are no citations that match your criteria.
FAQs for Human/Mouse HIF-1 alpha/HIF1A PE‑conjugated Antibody
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Q: Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
A: All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).
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Q: What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
A: The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.