Human/Mouse HIF-1 alpha/HIF1A Antibody

Name: Human/Mouse HIF-1 alpha/HIF1A Antibody
Brand: R&D Systems
SKU: MAB1935
Price: 157 USD

Catalog # MAB1935 | R&D Systems, Inc. a Bio-Techne Brand

Citations (8)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Immunohistochemistry, Intracellular Staining by Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 241812

Product Summary for Human/Mouse HIF-1 alpha/HIF1A Antibody

Immunogen

E. coli-derived recombinant human HIF-1 alpha/HIF1A
Arg575-Asn826
Accession # Q16665.1

Specificity

Recognizes human and mouse HIF-1 alpha/HIF1A in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Mouse HIF-1 alpha/HIF1A Antibody

HIF-1 alpha/HIF1A in Human Kidney Cancer Tissue.

HIF-1 alpha/HIF1A was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1935) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse HIF-1 alpha/HIF1A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast and kidney cancer tissue

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: CoCl₂‑treated MCF‑7 human breast cancer cell line, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name

Hypoxia Inducible Factor 1 Subunit Alpha

Alternate Names

BHLHE78, HIF 1A, HIF-1a, HIF1 alpha, HIF1A, MOP1, PASD8

Entrez Gene IDs

3091 (Human); 15251 (Mouse); 29560 (Rat)

Gene Symbol

HIF1A

UniProt

Q16665.1

Product Documents for Human/Mouse HIF-1 alpha/HIF1A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse HIF-1 alpha/HIF1A Antibody

For research use only

Citations for Human/Mouse HIF-1 alpha/HIF1A Antibody (8)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse HIF-1 alpha/HIF1A Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

WL Wang et al. (2022-02-18), microRNA-142 guards against autoimmunity byï¿½controllingï¿½Tregï¿½cell homeostasis and function PloS Biology, 2022-020(2):e3001552.
PMID: 35180231
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Y Sun et al. (2021-12-22), Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells Science Advances, 2021-17(52):eabj6877.
PMID: 34936448
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

JH Park et al. (2021-02-11), Tumor hypoxia represses &gamma&delta T cell-mediated antitumor immunity against brain tumors Nature Immunology, 2021-00(0).
PMID: 33574616
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

J Zhang et al. (2018-02-21), Celastrol mediates Th17 and Treg cell generation via metabolic signaling Biochem. Biophys. Res. Commun., 2018-00(0).
PMID: 29476742
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Liu G et al. (2015-02-17), Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1. Proc Natl Acad Sci U S A, 2015-0112(9):E957-65.
PMID: 25730867
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Mangiavini L et al. (2014-06-24), Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development. Dev Biol, 2014-0393(1):124-36.
PMID: 24972088
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Liu L et al. (2014-01-15), Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8. Int J Clin Exp Pathol, 2014-07(2):575-83.
PMID: 24551277
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Isayeva T et al. (2007-06-15), Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model. Cancer Res., 2007-067(12):5789-97.
PMID: 17575146

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