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Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody

Catalog # IC1935A | R&D Systems, Inc. a Bio-Techne Brand
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IC1935A

Key Product Details

Species Reactivity

Human, Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 241812

Product Summary for Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody

Immunogen

E. coli-derived recombinant human HIF-1 alpha
Arg575-Asn826
Accession # Q16665.1

Specificity

Recognizes human and mouse HIF-1 alpha in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody

Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line treated with CoCl2was stained with Mouse Anti-Human/Mouse HIF-1a APC-conjugated Monoclonal Antibody (Catalog # IC1935A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line, treated with CoCl2, fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using IC1935A in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name

Hypoxia Inducible Factor 1 Subunit Alpha

Alternate Names

BHLHE78, HIF 1A, HIF-1a, HIF1 alpha, HIF1A, MOP1, PASD8

Entrez Gene IDs

3091 (Human); 15251 (Mouse); 29560 (Rat)

Gene Symbol

HIF1A

UniProt

Product Documents for Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody

For research use only

Citations for Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody (4)

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Customer Reviews for Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody (2)

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  • Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: IC-21 mouse macrophage cell line and macrophage
    Species: Human
    Verified Customer | Posted 04/03/2021
    Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody IC1935A
  • Human/Mouse HIF-1 alpha APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: T regulatory cells
    Species: Mouse
    Verified Customer | Posted 08/03/2016
    Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody IC1935A

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FAQs for Human/Mouse HIF-1 alpha/HIF1A APC‑conjugated Antibody

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  • Q: Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.

    A: All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).

  • Q: What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?

    A: The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.

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