Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody

Catalog # IC1935C | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation = 482 and 564 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 241812

Product Summary for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody

Immunogen

E. coli-derived recombinant human HIF-1 alpha
Arg575-Asn826
Accession # Q16665.1

Specificity

Recognizes human and mouse HIF-1 alpha in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody

Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line treated with CoCl2was stained with Mouse Anti-Human/Mouse HIF-1a PerCP-conjugated Monoclonal Antibody (Catalog # IC1935C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line treated with 1 mM DFO overnight was stained with Mouse Anti-Human/Mouse HIF-1a PerCP-conjugated Monoclonal Antibody (Catalog # IC1935C, filled histogram) or isotype control antibody (IC002C, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line, treated with CoCl2, and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005), or treated with DFO and fixed and permeabilized with FlowX FoxP3/Transcription Factor Fix & Perm Kit (Catalog # FC012).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name

Hypoxia Inducible Factor 1 Subunit Alpha

Alternate Names

BHLHE78, HIF 1A, HIF-1a, HIF1 alpha, HIF1A, MOP1, PASD8

Entrez Gene IDs

3091 (Human); 15251 (Mouse); 29560 (Rat)

Gene Symbol

HIF1A

UniProt

Q16665.1

Product Documents for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody

For research use only

FAQs for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.

    A: All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).

  • Q: What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?

    A: The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.

Showing  1 - 2 of 2 FAQs Showing All
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