Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody
Catalog # IC1935C | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Customers Also Viewed
Product Summary for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody
Immunogen
Arg575-Asn826
Accession # Q16665.1
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody
Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line treated with CoCl2was stained with Mouse Anti-Human/Mouse HIF-1a PerCP-conjugated Monoclonal Antibody (Catalog # IC1935C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line treated with 1 mM DFO overnight was stained with Mouse Anti-Human/Mouse HIF-1a PerCP-conjugated Monoclonal Antibody (Catalog # IC1935C, filled histogram) or isotype control antibody (IC002C, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.Applications for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody
Intracellular Staining by Flow Cytometry
Sample: MCF‑7 human breast cancer cell line, treated with CoCl2, and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005), or treated with DFO and fixed and permeabilized with FlowX FoxP3/Transcription Factor Fix & Perm Kit (Catalog # FC012).
Formulation, Preparation and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HIF-1 alpha/HIF1A
The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
Product Documents for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody
Product Specific Notices for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody
For research use only
FAQs for Human/Mouse HIF-1 alpha/HIF1A PerCP‑conjugated Antibody
-
Q: Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
A: All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).
-
Q: What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
A: The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.