Human M-CSF APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC2161A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 26786
Product Specifications
Immunogen
E. coli-derived recombinant human M-CSF
Specificity
Detects human M-CSF.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human M-CSF APC-conjugated Antibody
Detection of M-CSF in PBMC by Flow Cytometry
Human peripheral blood mononuclear cells (PBMC) were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human M-CSF APC-conjugated Monoclonal Antibody (Catalog # IC2161A) or (B) isotype control antibody (Catalog # IC003A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human M-CSF APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human PBMC's were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human PBMC's were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: M-CSF
Long Name
Macrophage Colony Stimulating Factor
Alternate Names
CSF-1, CSF1, Lanimostim, MCSF
Gene Symbol
CSF1
Additional M-CSF Products
Product Documents for Human M-CSF APC-conjugated Antibody
Product Specific Notices for Human M-CSF APC-conjugated Antibody
For research use only
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