Does this Human Leptin R antibody detect the long form (ObRb) of Leptin R?

The immunogen sequence that this antibody was raised against corresponds to the extracellular domain of Leptin R. Because the extracellular domains of the long form and other isoforms of the receptor are identical, we expect that the antibody will detect all forms.

Human Leptin R Antibody

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB867

Citations (5)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Leptin R Phe22-Asp839 (predicted) Accession # P48357
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 52263
Specificity:	Detects human Leptin R in direct ELISAs and Western blots. In Western blots, cross reactivity with recombinant mouse Leptin R is observed.

View Full Technical Details for Human Leptin R Antibody

Applications for Human Leptin R Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/10⁶ cells	See below
Immunohistochemistry	8-25 µg/mL	Immersion fixed paraffin-embedded sections of human brain (cortex and hippocampus)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Leptin R Antibody

	Western Blot Detection of Recombinant Human Leptin R by Western Blot. Western blot shows 25 ng of Recombinant Human Leptin R Fc Chimera (Catalog # 389-LR) and Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Leptin R Monoclonal Antibody (Catalog # MAB867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Leptin R at approximately 155-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
	Flow Cytometry Detection of Leptin R in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Human Leptin R Monoclonal Antibody (Catalog # MAB867, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Leptin R

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-R_L) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-R_s) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript, lacking a transmembrane domain and potentially encoding a soluble form of the receptor, has also been described. OB-R_L transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-R_s transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. OB-R has been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype. Human OB-R long form encodes a 1165 amino acid (aa) precursor protein with a 22 aa signal peptide, an 819 aa extracellular domain, a 21 aa transmembrane domain and a 303 aa cytoplasmic domain. The extracellular domain of OB-R contain two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant soluble OB-R has been shown to bind Leptin with high affinity and is a potent Leptin antagonist.

Item	Value
References	Tartaglia, L.A. et al. (1995) Cell 83:1263. Cioffi, J.A. et al. (1996) Nature Medicine 2:585. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
Long Name	Leptin Receptor
Entrez Gene IDs	3953 (Human); 16847 (Mouse)
Alternate Names	B219, CD295, CD295 antigen, DB, DKFZp686B1731, LEP-R, LEPR, Leptin R, LeptinR, OB R, OB receptor, OB-R, OBRCD295, huB219, leptin receptor

Citations for Human Leptin R Antibody (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Leptin R Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Y Chen et al. (2022), Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis Theranostics, 12(3):1074-1096.
PMID: 35154475
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

K Watanabe et al. (2019), Leptin enhances cytokine/chemokine production by normal lung fibroblasts by binding to leptin receptor Allergol Int, 0(0).
PMID: 31029506
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Liang G et al. (2012), Normal leptin expression, lower adipogenic ability, decreased leptin receptor and hyposensitivity to Leptin in Adolescent Idiopathic Scoliosis. PLoS ONE, 7(5):e36648.
PMID: 22615788
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Hongo S et al. (2009), Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Am. J. Physiol. Endocrinol. Metab., 297(2):E474-82.
PMID: 19625677
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Francois F et al. (2007), The association of gastric leptin with oesophageal inflammation and metaplasia. Gut, 57(1):16-24.
PMID: 17761783

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Showing 1 - 5 of 5 citations Showing All

FAQs for Human Leptin R Antibody

Does this Human Leptin R antibody detect the long form (ObRb) of Leptin R?

The immunogen sequence that this antibody was raised against corresponds to the extracellular domain of Leptin R. Because the extracellular domains of the long form and other isoforms of the receptor are identical, we expect that the antibody will detect all forms.

Human Leptin R Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human Leptin R Antibody

Scientific Data Examples for Human Leptin R Antibody

Western Blot

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Leptin R

Citations for Human Leptin R Antibody (5)

FAQs for Human Leptin R Antibody

Product Documents for Human Leptin R Antibody

Product Datasheets

COA

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