Human Integrin alpha V beta 3 Antibody

Name: Human Integrin alpha V beta 3 Antibody
Brand: R&D Systems
SKU: MAB3050

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB3050

Citations (13)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	Human osteoclasts
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 23C6
Specificity:	Detects human Integrin alpha V beta 3.

View Full Technical Details for Human Integrin alpha V beta 3 Antibody

Applications for Human Integrin alpha V beta 3 Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	HUVEC human umbilical vein endothelial cells
Immunohistochemistry	Davies, J. et al. (1989) J. Cell Biol. 109:1817.
Immunoprecipitation	Davies, J. et al. (1989) J. Cell Biol. 109:1817.
Adhesion Blockade	Horton, M.A. et al. (1991) Exp. Cell Res. 195:368. Bates, R.C. et al. (1998) Cell Adhes. Commun. 6:21.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin alpha V beta 3

Integrin alpha V beta 3 together with alpha IIb beta ₃, constitutes the only known beta 3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta ₃/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg²⁺ or Mn²⁺ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow alpha V while the 685 aa human beta ₃ ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta ₃.

Item	Value
References	Hynes, R. O. (2002) Cell 110:673. Serini, G. et al. (2006) Exp. Cell Res. 312:651. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88. Xiong, J. et al. (2001) Science 294:339. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415. Djaffar, I. et al. (1994) Biochem. J. 300:67. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433. Faccio, R. et al. (2003) J. Clin. Invest. 111:749. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
Entrez Gene IDs	3685 (Human)
Alternate Names	CD51, CD51 antigen, Integrin alpha V beta 3, MSK8, VNRADKFZp686A08142, Vitronectin receptor subunit alpha, antigen identified by monoclonal L230, integrin alpha-V, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

Citations for Human Integrin alpha V beta 3 Antibody (13)

Showing 1 - 10 of 13 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human Integrin alpha V beta 3 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

SM Shin et al. (2020), Direct targeting of oncogenic RAS mutants with a tumor-specific cytosol-penetrating antibody inhibits RAS mutant-driven tumor growth Sci Adv, 6(3):eaay2174.
PMID: 31998840
Species: Human
Sample Types: Whole Cells
Applications: ICC

E Peterman et al. (2019), The post-abscission midbody is an intracellular signaling organelle that regulates cell proliferation Nat Commun, 10(1):3181.
PMID: 31320617
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

YP Jin et al. (2018), HLA Class II-Triggered Signaling Cascades Cause Endothelial Cell Proliferation and Migration: Relevance to Antibody-Mediated Transplant Rejection J. Immunol., 0(0).
PMID: 29475988
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CS Fan et al. (2018), Osteopontin-integrin engagement induces HIF-1?-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer Oncotarget, 9(4):4998-5015.
PMID: 29435158
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

HA Mena et al. (2018), Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo Stem Cell Res Ther, 9(1):120.
PMID: 29720269
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

A Elnaggar et al. (2017), AlphaVBeta3 Integrin expression within uterine endometrium in unexplained infertility: a prospective cohort study BMC Womens Health, 17(1):90.
PMID: 28950833
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

K Miyano et al. (2017), cRGD peptide installation on cisplatin-loaded nanomedicines enhances efficacy against locally advanced head and neck squamous cell carcinoma bearing cancer stem-like cells J Control Release, 0(0).
PMID: 28666729
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Su C et al. (2015), Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy. J Immunol, 195(7):3355-64.
PMID: 26304992
Species: Human
Sample Types: Whole Cells
Applications: IHC

Kasonga A et al. (2015), Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro. PLoS ONE, 10(4):e0125145.
PMID: 25867515
Zhang P et al. (2015), C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation. Sci Rep, 5(0):9275.
PMID: 25792190
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Dotterweich J et al. (2014), Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells. Cell Commun Signal, 12(0):36.
PMID: 24965524
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

DeNiro M et al. (2013), The nexus between VEGF and NFkappaB orchestrates a hypoxia-independent neovasculogenesis. PLoS ONE, 8(3):e59021.
PMID: 23533599
Species: Human
Sample Types: Whole Cells
Applications: ICC

Suri SS et al. (2009), The role of RGD-tagged helical rosette nanotubes in the induction of inflammation and apoptosis in human lung adenocarcinoma cells through the P38 MAPK pathway. Biomaterials, 30(17):3084-90.
PMID: 19250666

There are no citations that match your criteria.

Showing 1 - 10 of 13 citations Showing All

Product Documents for Human Integrin alpha V beta 3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Human Integrin alpha V beta 3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased